Team:Aachen/content

Uberschrift

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=Schwaneberg= ==Creation of glycerol-stocks of Minigene 32 and Megagene 51== '''Sarah''' :2 Eppis: * take 1mL of: ** Minigene 32 O.N. ** Megagene 51 O.N. * add 1ml of Glycerol 50% stock solution to each plasmid *store at -80°C ==Competent BY4742+KO cells from Leu(-) O.N. with kit-protocol== '''Sarah, Lea''' * 4,5ml YPD-Medium * add 500µL of O.N. * incubate at 30°C for ~4h (shaking) :OD was measured: 1,2 * 4 mL culture was centrifuged (500 x g, 4min), supernatant discarded * pellet was resuspended in 8mL EZ1, centrifuged, supernatant discarded * pellet was resuspended in 0,4mL EZ2 * solutiohn was distributed into 1,5mL tubes (50µL per tube) * cells (BY+PAA1) were frozen slowly at -80°C == == Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Aenean commodo ligula eget dolor. Aenean massa. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Donec quam felis, ultricies nec, pellentesque eu, pretium quis, sem. Nulla consequat massa quis enim. Donec pede justo, fringilla vel, aliquet nec, vulputate eget, arcu. In enim justo, rhoncus ut, imperdiet a, venenatis vitae, justo. Nullam dictum felis eu pede mollis pretium. Integer tincidunt. Cras dapibus. Vivamus elementum semper nisi. Aenean vulputate eleifend tellus. Aenean leo ligula, porttitor eu, consequat vitae, elei

Uberschrift

Schwaneberg

Creation of glycerol-stocks of Minigene 32 and Megagene 51

Sarah

2 Eppis:
  • take 1mL of:
    • Minigene 32 O.N.
    • Megagene 51 O.N.
  • add 1ml of Glycerol 50% stock solution to each plasmid
  • store at -80°C

Competent BY4742+KO cells from Leu(-) O.N. with kit-protocol

Sarah, Lea

  • 4,5ml YPD-Medium
  • add 500µL of O.N.
  • incubate at 30°C for ~4h (shaking)
OD was measured: 1,2
  • 4 mL culture was centrifuged (500 x g, 4min), supernatant discarded
  • pellet was resuspended in 8mL EZ1, centrifuged, supernatant discarded
  • pellet was resuspended in 0,4mL EZ2
  • solutiohn was distributed into 1,5mL tubes (50µL per tube)
  • cells (BY+PAA1) were frozen slowly at -80°C