This page shows KAIT_JAPAN's contribution to the Fifth International InterLaboratory Measurement Study the fifth Interlab.
There are two goals of fifth Interlab:
1. Reducing lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD using two orthogonal approaches.
2. Converting between absorbance of cells to absorbance of a known concentration of beads and CFUs from the sample.
Calibration 1: OD 600 Reference point
Table 1. Absorbance 600 nm of LUDOX-S40 and H₂O
Calibration 2: Particle Standard Curve
Fig 1. Particle Standard Curve
Fig 2. Particle Standard Curve(log scale)
Calibration 3: Fluorescence standard curve
Fig 3. Fluorescein Standard Curve
Fig 4. Fluorescein Standard Curve (log scale)
Table 2. Fluorescence Raw Readings
Table 3. Abs600 Raw Readings