Team:KUAS Korea/DryLab/Overview
Modeling Overview |
Does altruistic character benefit the survival?
: cost and benefit of altruism – and how it relates to the evolutionary strategy of organisms.
- Cost(c): the metabolic cost of the expression of β-glucosidase (transcription, translation, surface display mechanism)(only cooperator)
Benefit(δ): the consumption of the energy source (glucose)(both the cheater and the cooperator)
- The goal of our modeling is to compare the cost(c) and benefit(δ) of the cell’s altruistic activity. In order to do that, we’ve designed the most basic biological situation with two types of E. coli: the cheater and the cooperator. The key point of this model is that the two strains compete for a single type of energy source- the glucose(which is marked as ‘public good’ in the figure). However, the glucose is scarce in the environment; it is actually not provided in the media but can only be generated by the hydrolytic activity of β-glucosidase on cellobiose. This is the point where the cooperator shows it’s altruism. By displaying the β-glucosidase on the cell surface, the cooperator both benefits itself and the cheater. This process also requires some cost, such as the metabolic cost of expressing the β-glucosidase, and the energy required for its activity. On the other hand, the cheater only receives benefits from the cooperator’s activity providing nothing in return.
- In our modeling, we’ve established mathematical equations for estimating the growth rate of each strain. Then, we compared the value of c and δ at various growth conditions to figure out the effect of altruism on the survival.
- Several assumptions were made prior to the modeling.
- The higher total cell density will increase the relative growth rate of the cooperator. - If the total cell density is high, it is more likely that there are more cooperators which leads to the bigger production of glucose. Less scarcity of the glucose loosens the competition of cooperator and cheater, which means the effect of cost on survival becomes smaller while the effect of the benefit becomes bigger.
- The growth rate of the cooperator will increase as its initial relative population increase. - The initial population condition will be one of the key variables of this model. If the cooperator to cheater ratio is high, cooperator will gain enough benefit from their cellobiose-cleavage activity. In this case, the metabolic cost of β-glucosidase expression is endurable since the cooperator itself needs the β-glucosidase activity anyway in order to obtain glucose as the energy source. However, when the cooperator to cheater ration is low, cooperator will not get enough benefit compared to the investment(cost) it has made. This will lower the survival rate of the cooperator while making cheater easy to survive.
- By dry-lab modeling of our project, we can...
- Estimate the results of wet-lab experiment and compare the data.
- Expand the project to more sophisticated evolutionary situation that is closer to real life but yet hard to implement in wet-lab.
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