Troubleshooting -- Wiki course
Characterization of Parts from NTHU_Formosa
We characterize the part of NTHU_Formosa team. These inserted vectors, including pCAG-GBP, pTRE3G-LuxAB, pCMV-LuxCDEfrp, pTRE3G-mCherry, are to produce receptor, enzyme, substrate, which are essential in the process of emitting the light. We helped with NTHU_Formosa to check if these vectors are correct. The results in the figure below shows these vectors are connected correctly.
Bidirectional collaboration with NTHU_Formosa
After inducing our E. coli, we wanted double check that our constructs were correctly expressed. To do this, we invited members of team NTHU_Formosa to come to our lab on October 6th, where they spent an afternoon running an SDS-PAGE of Enterocin B and Enterocin 96. The results are shown in the figures below. Thanks to NTHU_Formosa’s help, we confirmed that our peptides were being expressed correctly.
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