Team:TJU China/Parts

<!DOCTYPE >

Parts Overview
We submitted 38 parts in total this years, including basic parts and composite parts. As our projects are about CRISPR, most of our parts are related to the CRISPR system. Some of the parts are DNA template for sgRNA of ABCB1, GFP, RED and EGFP. Some of them are DNA template which can transfer into crRNA. Except for three mitochondrial-targeted sequences, there are also sequences of composite parts, which consist of two or more elements below: promoter, RBS, ArsR gene, smURFP and BV.
Part number Type Description Designer Length
BBa_K2644001 Composite Fluorescent protein gene smURFP with enzyme gene BV Yiran Cheng 1173
BBa_K2644002 Composite Total sequence of the plasmid to detect As3+ Yiran Cheng 1678
BBa_K2644003 Composite ArsR gene with ArsR promoter and RBS Yiran Cheng 472
BBa_K2644004 Composite Sequence of ArsR promoter and RBS Yiran Cheng 118
BBa_K2644007 Coding smURFP protein Yiran Cheng 402
BBa_K2644009 Composite dCas9+ω Yiran Cheng 4383
BBa_K2644010 Composite J23104+ArsR Yiran Cheng 415
BBa_K2644101 Coding FnCas12a protein Xinyi Wang 3903
BBa_K2644102 Coding LbCas12a protein Xinyi Wang 3471
BBa_K2644103 Coding GFP protein Xinyi Wang 720
BBa_K2644104 Coding RED protein Xinyi Wang 678
BBa_K2644105 Composite T7+primer for the DNA template of crRNA of DNMT(FnCasa12a) Xinyi Wang 67
BBa_K2644106 Composite T7+primer for the DNA template of crRNA of EGFP(LbCas12a) Xinyi Wang 66
BBa_K2644107 Composite T7+primer for the DNA template of crRNA of GFP Xinyi Wang 68
BBa_K2644108 Composite T7+primer for the DNA template of crRNA of GFP Xinyi Wang 68
BBa_K2644109 Composite T7+primer for the DNA template of crRNA of RED Xinyi Wang 68
BBa_K2644110 Composite T7+primer for the DNA template of crRNA of RED Xinyi Wang 68
BBa_K2644111 Composite T7+primer for the DNA template of crRNA of RED Xinyi Wang 68
BBa_K2644112 Composite T7+primer for the DNA template of crRNA of GFP Xinyi Wang 25
BBa_K2644113 Basic DNA template of crRNA of LbCas12a Xinyi Wang 45
BBa_K2644114 Basic DNA template of crRNA of LbCas12a Xinyi Wang 45
BBa_K2644115 Basic DNA template of crRNA of LbCas12a Xinyi Wang 45
BBa_K2644116 Basic DNA template of crRNA of LbCas12a Xinyi Wang 45
BBa_K2644117 Basic DNA template of crRNA of FnCas12a Xinyi Wang 43
BBa_K2644118 Basic DNA template of crRNA of FnCas12a Xinyi Wang 43
BBa_K2644119 Basic DNA template of crRNA of FnCas12a Xinyi Wang 43
BBa_K2644120 Basic DNA template of crRNA of FnCas12a Xinyi Wang 43
BBa_K2644121 Basic DNA template of crRNA of FnCas12a Xinyi Wang 45
BBa_K2644122 Primer The primer of crRNA for EGFP(LbCas12a) Xinyi Wang 41
BBa_K2644123 Primer The primer of crRNA for DNMT（FnCas12a） Xinyi Wang 42
BBa_K2644125 Primer The primer of crRNA for RED Xinyi Wang 43
BBa_K2644126 Primer The primer of crRNA for RED Xinyi Wang 43
BBa_K2644127 Primer The primer of crRNA for RED Xinyi Wang 43
BBa_K2644128 Primer The primer of crRNA for GFP Xinyi Wang 43
BBa_K2644129 Primer The primer of crRNA for GFP Xinyi Wang 43
BBa_K2644201 Basic The DNA template for sgRNA of ABCB1 Xiaoyu Yang 119
BBa_K2644202 Basic The DNA template for sgRNA of ABCB1 Xiaoyu Yang 122
BBa_K2644203 Basic The DNA template for sgRNA of ABCB1 Xiaoyu Yang 123
BBa_K2644204 Basic The DNA template for sgRNA of EGFP Xiaoyu Yang 123
BBa_K2644205 Basic The DNA template for sgRNA of EGFP Xiaoyu Yang 128
BBa_K2644206 Basic The DNA template for sgRNA of EGFP Xiaoyu Yang 123
BBa_K2644207 Basic The DNA template for sgRNA of RED Xiaoyu Yang 128
BBa_K2644208 Basic The DNA template for sgRNA of RED Xiaoyu Yang 128
BBa_K2644209 Basic The DNA template for sgRNA of RED Xiaoyu Yang 123
BBa_K2644210 Basic GFP protein Xiaoyu Yang 720
BBa_K2644212 Basic The DNA template for sgRNA of EGFP Xiaoyu Yang 123
BBa_K2644213 Basic The DNA template for sgRNA of EGFP Xiaoyu Yang 123
BBa_K2644301 Basic The DNA template of Cox8A Junjing Chen 87
BBa_K2644302 Basic The DNA template of SOD2 Junjing Chen 102
BBa_K2644303 Basic The DNA template of ATP5B Junjing Chen 159
BBa_K2644304 Basic The DNA template of sgRNA for Cox1 Junjing Chen 128
BBa_K2644305 Basic The DNA template of sgRNA for Cox1 Junjing Chen 128
BBa_K2644306 Basic The DNA template of sgRNA for Cox1 Junjing Chen 128
BBa_K2644307 Basic The DNA template of sgRNA for Cox2 Junjing Chen 128
BBa_K2644308 Basic The DNA template of sgRNA for Cox2 Junjing Chen 128
BBa_K2644309 Basic The DNA template of sgRNA for Cox2 Junjing Chen 128
BBa_K2644310 Basic The DNA template of sgRNA for ND5 Junjing Chen 128
BBa_K2644311 Basic The DNA template of sgRNA for ND5 Junjing Chen 128
BBa_K2644312 Basic The DNA template of sgRNA for ND5 Junjing Chen 128
BBa_K2644313 Basic The DNA template of sgRNA for TRNA1 Junjing Chen 128
BBa_K2644314 Basic The DNA template of sgRNA for TRNA1 Junjing Chen 128
BBa_K2644315 Basic The DNA template of sgRNA for TRNA1 Junjing Chen 128
Basic Parts
Part number Type Description Designer Length
BBa_K2644113 Basic DNA template of crRNA of LbCas12a Xinyi Wang 45
BBa_K2644114 Basic DNA template of crRNA of LbCas12a Xinyi Wang 45
BBa_K2644115 Basic DNA template of crRNA of LbCas12a Xinyi Wang 45
BBa_K2644116 Basic DNA template of crRNA of LbCas12a Xinyi Wang 45
BBa_K2644117 Basic DNA template of crRNA of FnCas12a Xinyi Wang 43
BBa_K2644118 Basic DNA template of crRNA of FnCas12a Xinyi Wang 43
BBa_K2644119 Basic DNA template of crRNA of FnCas12a Xinyi Wang 43
BBa_K2644120 Basic DNA template of crRNA of FnCas12a Xinyi Wang 43
BBa_K2644121 Basic DNA template of crRNA of FnCas12a Xinyi Wang 45
BBa_K2644201 Basic The DNA template for sgRNA of ABCB1 Xiaoyu Yang 119
BBa_K2644202 Basic The DNA template for sgRNA of ABCB1 Xiaoyu Yang 122
BBa_K2644203 Basic The DNA template for sgRNA of ABCB1 Xiaoyu Yang 123
BBa_K2644204 Basic The DNA template for sgRNA of EGFP Xiaoyu Yang 123
BBa_K2644205 Basic The DNA template for sgRNA of EGFP Xiaoyu Yang 128
BBa_K2644206 Basic The DNA template for sgRNA of EGFP Xiaoyu Yang 123
BBa_K2644207 Basic The DNA template for sgRNA of RED Xiaoyu Yang 128
BBa_K2644208 Basic The DNA template for sgRNA of RED Xiaoyu Yang 128
BBa_K2644209 Basic The DNA template for sgRNA of RED Xiaoyu Yang 123
BBa_K2644210 Basic GFP protein Xiaoyu Yang 720
BBa_K2644212 Basic The DNA template for sgRNA of EGFP Xiaoyu Yang 123
BBa_K2644213 Basic The DNA template for sgRNA of EGFP Xiaoyu Yang 123
BBa_K2644301 Basic The DNA template of Cox8A Junjing Chen 87
BBa_K2644302 Basic The DNA template of SOD2 Junjing Chen 102
BBa_K2644303 Basic The DNA template of ATP5B Junjing Chen 159
BBa_K2644304 Basic The DNA template of sgRNA for Cox1 Junjing Chen 128
BBa_K2644305 Basic The DNA template of sgRNA for Cox1 Junjing Chen 128
BBa_K2644306 Basic The DNA template of sgRNA for Cox1 Junjing Chen 128
BBa_K2644307 Basic The DNA template of sgRNA for Cox2 Junjing Chen 128
BBa_K2644308 Basic The DNA template of sgRNA for Cox2 Junjing Chen 128
BBa_K2644309 Basic The DNA template of sgRNA for Cox2 Junjing Chen 128
BBa_K2644310 Basic The DNA template of sgRNA for ND5 Junjing Chen 128
BBa_K2644311 Basic The DNA template of sgRNA for ND5 Junjing Chen 128
BBa_K2644312 Basic The DNA template of sgRNA for ND5 Junjing Chen 128
BBa_K2644313 Basic The DNA template of sgRNA for TRNA1 Junjing Chen 128
BBa_K2644314 Basic The DNA template of sgRNA for TRNA1 Junjing Chen 128
BBa_K2644315 Basic The DNA template of sgRNA for TRNA1 Junjing Chen 128
Composite Parts
Part number Type Description Designer Length
BBa_K2644001 Composite Fluorescent protein gene smURFP with enzyme gene BV Yiran Cheng 1173
BBa_K2644002 Composite Total sequence of the plasmid to detect As3+ Yiran Cheng 1678
BBa_K2644004 Composite Sequence of ArsR promoter and RBS Yiran Cheng 118
BBa_K2644009 Composite dCas9+ω Yiran Cheng 4383
BBa_K2644010 Composite J23104+ArsR Yiran Cheng 415
BBa_K2644105 Composite T7+primer for the DNA template of crRNA of DNMT(FnCasa12a) Xinyi Wang 67
BBa_K2644106 Composite T7+primer for the DNA template of crRNA of EGFP(LbCas12a) Xinyi Wang 66
BBa_K2644107 Composite T7+primer for the DNA template of crRNA of GFP Xinyi Wang 68
BBa_K2644108 Composite T7+primer for the DNA template of crRNA of GFP Xinyi Wang 68
BBa_K2644109 Composite T7+primer for the DNA template of crRNA of RED Xinyi Wang 68
BBa_K2644110 Composite T7+primer for the DNA template of crRNA of RED Xinyi Wang 68
BBa_K2644111 Composite T7+primer for the DNA template of crRNA of RED Xinyi Wang 68
BBa_K2644112 Composite T7+primer for the DNA template of crRNA of GFP Xinyi Wang 25
Part Collection
Part number Type Description Designer Length
BBa_K2644001 Composite Fluorescent protein gene smURFP with enzyme gene BV Yiran Cheng 1173
BBa_K2644002 Composite Total sequence of the plasmid to detect As3+ Yiran Cheng 1678
BBa_K2644003 Composite ArsR gene with ArsR promoter and RBS Yiran Cheng 472
BBa_K2644004 Composite Sequence of ArsR promoter and RBS Yiran Cheng 118
BBa_K2644007 Coding smURFP protein Yiran Cheng 402
BBa_K2644010 Composite J23104+ArsR Yiran Cheng 415
BBa_K2644105 Composite T7+primer for the DNA template of crRNA of DNMT(FnCasa12a) Xinyi Wang 67
BBa_K2644106 Composite T7+primer for the DNA template of crRNA of EGFP(LbCas12a) Xinyi Wang 66
BBa_K2644107 Composite T7+primer for the DNA template of crRNA of GFP Xinyi Wang 68
BBa_K2644108 Composite T7+primer for the DNA template of crRNA of GFP Xinyi Wang 68
BBa_K2644109 Composite T7+primer for the DNA template of crRNA of RED Xinyi Wang 68
BBa_K2644110 Composite T7+primer for the DNA template of crRNA of RED Xinyi Wang 68
BBa_K2644111 Composite T7+primer for the DNA template of crRNA of RED Xinyi Wang 68
BBa_K2644113 Basic DNA template of crRNA of LbCas12a Xinyi Wang 45
BBa_K2644114 Basic DNA template of crRNA of LbCas12a Xinyi Wang 45
BBa_K2644115 Basic DNA template of crRNA of LbCas12a Xinyi Wang 45
BBa_K2644116 Basic DNA template of crRNA of LbCas12a Xinyi Wang 45
BBa_K2644117 Basic DNA template of crRNA of FnCas12a Xinyi Wang 43
BBa_K2644118 Basic DNA template of crRNA of FnCas12a Xinyi Wang 43
BBa_K2644122 Primer The primer of crRNA for EGFP(LbCas12a) Xinyi Wang 41
BBa_K2644125 Primer The primer of crRNA for RED Xinyi Wang 43
BBa_K2644127 Primer The primer of crRNA for RED Xinyi Wang 43
BBa_K2644128 Primer The primer of crRNA for GFP Xinyi Wang 43
BBa_K2644129 Primer The primer of crRNA for GFP Xinyi Wang 43
BBa_K2644201 Basic The DNA template for sgRNA of ABCB1 Xiaoyu Yang 119
BBa_K2644202 Basic The DNA template for sgRNA of ABCB1 Xiaoyu Yang 122
BBa_K2644203 Basic The DNA template for sgRNA of ABCB1 Xiaoyu Yang 123
BBa_K2644204 Basic The DNA template for sgRNA of EGFP Xiaoyu Yang 123
BBa_K2644205 Basic The DNA template for sgRNA of EGFP Xiaoyu Yang 128
BBa_K2644206 Basic The DNA template for sgRNA of EGFP Xiaoyu Yang 123
BBa_K2644207 Basic The DNA template for sgRNA of RED Xiaoyu Yang 128
BBa_K2644208 Basic The DNA template for sgRNA of RED Xiaoyu Yang 128
BBa_K2644209 Basic The DNA template for sgRNA of RED Xiaoyu Yang 123
BBa_K2644210 Basic GFP protein Xiaoyu Yang 720
BBa_K2644212 Basic The DNA template for sgRNA of EGFP Xiaoyu Yang 123
BBa_K2644301 Basic The DNA template of Cox8A Junjing Chen 87
BBa_K2644302 Basic The DNA template of SOD2 Junjing Chen 102
BBa_K2644303 Basic The DNA template of ATP5B Junjing Chen 159
Biobrick improvement and characterization for existing parts
We need to observe fluorescence in our projects. To get better result, we made a part BBa_K2644001 which is better than the original part BBa_K2328000. We took two existing parts from this year’s plate because we need them and added new characterization to them, one is BBa_K1123005 , another one is BBa_J33201.