Difference between revisions of "Team:ICT-Mumbai/InterLab"

This year's InterLab study was conducted to answer the following question:
Can we reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD?

Team ICT-Mumbai participated in the interlab study this year. The plate reader protocol of the plate reader study as given on the measurements page of the iGEM website was strictly followed (the protocol can be downloaded here) and resulting data added to the provided Excel sheet which was subsequently uploaded (the Excel sheet can be downloaded here ).
The figures given below show the obtained results for the cell measurement protocol of this year’s interlab study.

Each test device used in the study comprises of the same gene and RBS, but has a different constitutive promoter from the Anderson family of promoters. Each promoter thus has a different level of expression of the GFP protein. Test device 1 worked better than expected whereas test device 5 failed to work as expected. It is seen that the common sense criteria are being followed, i.e., both the fluorescence and absorbance values are increasing with time for all devices and the positive control exhibits a better fluorescence and absorbance than the negative control. It was also observed that both, the fluorescence/OD and fluorescence/particle values decreased after 6 hours.

The results of the CFU protocol were uploaded in the form given on the interlab page of the iGEM website. The results obtained are shown below.