Dr. Ng is the mother of NCKU-iGEM team. Since the establishment of NCKU-iGEM 2016, Dr. Ng have brought synthetic biology as well as iGEM to the flashlight of NCKU campus. Dr. Ng always inspires team members by asking us some critical questions without telling us the answer directly. She gave us advice from every aspect. She initially gave us many critical suggestions during the brainstorming. Except for teaching us knowledge about synthetic biology, she always reminds us to think more about the real application of our project.

Various useful advice was given by her. She emphasized the advantage of protein production by E. coli, she also suggested us to study more on high cell density fermentation. Without her timely advice, our team cannot improve our project.

Our PI, Dr. Huang work as an assistant professor under the institute of Basic Medical Sciences of National Cheng Kung Univercity. His major field are Bacteriology, Bacteria Genetics, and Molecular Biology.

We invited Dr. Huang to attend our first presentation. During our first presentation, he provided us a lot of useful feedback and we could feel the intention of Dr. Huang. So, our team think that Dr. Huang could guide us for all our project and we wanted to invite him as our PI. Luckily, we have succeed!

From Dr. Huang's first day as our PI, he did a lots for us. Firstly, he provided us all the resources that we needed or could make us convenient. Secondly, he gave us many ideas when we have met difficulties or junction in our progress whether it is dry lab or wet lab. We are really appreciate to what he have told and taught to us.

This year, our project is related to carbon sequestration. Therefore, we grab the chance to discuss our idea with Professor Bieng-Zih Hsieh. Professor Bieng-Zih Hsieh is an associate professor from the Department of Resources Engineering. One of his research topics is about CO2 sequestration, which is a topic that related to our project, the difference is he employs a physical method in this aspect, while we use a biological method.

At first, we define our project as carbon capture. However, he introduced the idea of carbon utilization to us as he thought it is more suitable for our project. Professor Hsieh introduced some carbon capture or utilization strategies that implemented in the industry currently and suggest us to do more research on those methods. He also remind us to compare our system with other biological approach to figure out our strength and weakness.

From Dr. Huang's first day as our PI, he did a lots for us. Firstly, he provided us all the resources that we needed or could make us convenient. Secondly, he gave us many ideas when we have met difficulties or junction in our progress whether it is dry lab or wet lab. We are really appreciate to what he have told and taught to us.

Prof. Chao Chung, Peng is an assistant professor of Department of Aeronautics and Astronautics and International Bachelor Degree Program on Energy. He used to work in China steel corp. so he gave us a chance to contact CSC.

we met up with prof. Chung to get some suggestions which more proving our direction of our projects. Prof. Chung is proficient in energy field, different from giving us experimental technical advice, he gave us more about suggestion for action on device and idea.

He thinks that when we look for our advantages, we can take apart each of our research processes and experimental steps, and find every possible point that is more inefficient. The benefit of the whole project is very great. For example, what kind of way we can use to let every E.coli to eat most CO2, the way we replace our cultured E.coli. It is true that there are every existing way we can think more about how we can improve. Also, professor tell us we must be very understandable about our project, it includes our advantages, disadvantages, potential threat, our whole project process, and the most optimum environment we should provide for our E.coli. The more we realize our project, we can find more possibility of development. After talking with professor, we inspired more ideas and found direction about our project more clearly.

He gave us some advices in engineering pointview. Therefore, we tried to rewirte a big scope of our project and defined the points that we have to fulfill.

We had an opportunity to speak with Dr. Huang Chieh-Chen from National Chung Hsing University (NCHU) in the Asia Pacific Conference, whose work includes the reverse TCA cycle of engineered carbon capturing E. coli. Upon our discussion, Dr. Huang raised the issue of the low efficiency of one of our enzymes, Rubisco and suggested that we culture our engineered E.coli in an anaerobic condition. Since Rubisco, the ribulose-1,5-biphosphate carboxylase/oxygenase is an enzyme catalyzing the reaction of either carbon dioxide or oxygen with ribulose-1,5-biphosphate (RuBP) as the name itself suggests, putting our system in an aerobic condition would significantly lower the efficiency of capturing carbon dioxide due to the similarities between gaseous carbon dioxide and oxygen.

We had an opportunity to speak with Dr. Huang Chieh-Chen from National Chung Hsing University (NCHU) in the Asia Pacific Conference, whose work includes the reverse TCA cycle of engineered carbon capturing E. coli. Upon our discussion, Dr. Huang raised the issue of the low efficiency of one of our enzymes, Rubisco and suggested that we culture our engineered E.coli in an anaerobic condition. Since Rubisco, the ribulose-1,5-biphosphate carboxylase/oxygenase is an enzyme catalyzing the reaction of either carbon dioxide or oxygen with ribulose-1,5-biphosphate (RuBP) as the name itself suggests, putting our system in an aerobic condition would significantly lower the efficiency of capturing carbon dioxide due to the similarities between gaseous carbon dioxide and oxygen.