Difference between revisions of "Team:NCKU Tainan/Notebook"

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<h1>Notebook</h1>
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<p> Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.</p>
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                            <h1 class="head2">Notebook</h1>
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                            <h3>Construction of Pasr and Pgada</h3>
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                            <p class="pcontent">
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                                4/26~4/27</br>
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                                <ul>
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                                    <li>PCR Pasr and Pgada from E. coli MG1655 chromosome. Confirmed and extracted
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                                        products by DNA gel. </li>
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                                </ul>
  
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                                4/28</br>
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                                <ul>
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                                    <li>Digested the pSB1C3-sfGFP plasmid. Ligase the plasmid with Pasr / Pgada and
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                                        RiboJ by PCR. Confirmed by DNA gel electrophoresis. Transformed the gene in DH5
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                                        alpha and spread the bacteria on LB plates.</li>
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                                </ul>
  
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                                4/30~5/1</br>
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<h3>What should this page have?</h3>
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                                <ul>
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<ul>
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                                    <li>Confirmed the transformation by colony PCR ,enzyme digestion and DNA gel
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<li>Chronological notes of what your team is doing.</li>
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                                        electrophoresis. Selected the correct colonies for sequencing and cultured them
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<li> Brief descriptions of daily important events.</li>
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                                        on LB plates.</li>
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<li>Pictures of your progress. </li>
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                                </ul>
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<li>Mention who participated in what task.</li>
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</ul>
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                                5/9</br>
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                                <ul>
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                                    <li>Store the correct colonies in glycerol in -80O C.</li>
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                                </ul>
  
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                                6/1</br>
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<div class="highlight decoration_A_full">
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                                <ul>
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<h3>Inspiration</h3>
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                                        <li>Transformed each plasmid into BL21(DE3)</li>
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<p>You can see what others teams have done to organize their notes:</p>
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                                    </ul>
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<ul>
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                            </p>
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<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
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                        </div>
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<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
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                    </div>
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<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
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                </div>
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<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
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            </div>
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</ul>
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        </div>
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Revision as of 04:59, 22 September 2018

Notebook

Construction of Pasr and Pgada

4/26~4/27

  • PCR Pasr and Pgada from E. coli MG1655 chromosome. Confirmed and extracted products by DNA gel.
4/28
  • Digested the pSB1C3-sfGFP plasmid. Ligase the plasmid with Pasr / Pgada and RiboJ by PCR. Confirmed by DNA gel electrophoresis. Transformed the gene in DH5 alpha and spread the bacteria on LB plates.
4/30~5/1
  • Confirmed the transformation by colony PCR ,enzyme digestion and DNA gel electrophoresis. Selected the correct colonies for sequencing and cultured them on LB plates.
5/9
  • Store the correct colonies in glycerol in -80O C.
6/1
  • Transformed each plasmid into BL21(DE3)

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igem.ncku.tainan@gmail.com
No.1, Daxue Rd., East Dist., Tainan City 701, Taiwan (R.O.C.)