Notebook

Construction of Pasr and Pgada

4/26~4/27

• PCR Pasr and Pgada from E. coli MG1655 chromosome. Confirmed and extracted products by DNA gel.

4/28

• Digested the pSB1C3-sfGFP plasmid. Ligase the plasmid with Pasr / Pgada and RiboJ by PCR. Confirmed by DNA gel electrophoresis. Transformed the gene in DH5 alpha and spread the bacteria on LB plates.

4/30~5/1

• Confirmed the transformation by colony PCR ,enzyme digestion and DNA gel electrophoresis. Selected the correct colonies for sequencing and cultured them on LB plates.

5/9

• Store the correct colonies in glycerol in -80O C.

6/1

• Transformed each plasmid into BL21(DE3)