Oscarliu117 (Talk | contribs) |
Oscarliu117 (Talk | contribs) |
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<a class="list-group-item list-group-item-action" href="#list-item-1">PCR</a> | <a class="list-group-item list-group-item-action" href="#list-item-1">PCR</a> | ||
<a class="list-group-item list-group-item-action" href="#list-item-2">Plasmid Construction</a> | <a class="list-group-item list-group-item-action" href="#list-item-2">Plasmid Construction</a> | ||
− | <a class="list-group-item list-group-item-action" href="#list-item-3">PCR Clean-Up & Gel Extraction</a> | + | <a class="list-group-item list-group-item-action" href="#list-item-3">PCR Clean-Up & Gel |
+ | Extraction</a> | ||
<a class="list-group-item list-group-item-action" href="#"><i class="fa fa-arrow-up fa-1x" | <a class="list-group-item list-group-item-action" href="#"><i class="fa fa-arrow-up fa-1x" | ||
aria-hidden="true"></i></a> | aria-hidden="true"></i></a> | ||
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</table> | </table> | ||
<p class="pcontent"> | <p class="pcontent"> | ||
− | |||
− | |||
<ol start="2"> | <ol start="2"> | ||
<li>Confirm the size of the digested product by gel electrophoresis.</li></br></br> | <li>Confirm the size of the digested product by gel electrophoresis.</li></br></br> | ||
<li>Gel purification of the target size.</li> | <li>Gel purification of the target size.</li> | ||
</ol> | </ol> | ||
− | + | </br> | |
</p> | </p> | ||
− | |||
− | |||
− | |||
</div> | </div> | ||
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<div id="list-item-2"> | <div id="list-item-2"> | ||
− | |||
</br> | </br> | ||
<div class="row"> | <div class="row"> | ||
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<ol start="3"> | <ol start="3"> | ||
<li>Confirm the size of the digested product by gel electrophoresis.</li> | <li>Confirm the size of the digested product by gel electrophoresis.</li> | ||
− | |||
<li>Gel purification of the target size.</li> | <li>Gel purification of the target size.</li> | ||
− | |||
<li>Ligation</li> | <li>Ligation</li> | ||
− | |||
</ol> | </ol> | ||
</p> | </p> | ||
− | <table class="card card-body"> | + | <table class="card card-body PC2"> |
<tr> | <tr> | ||
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</br> | </br> | ||
<p class="pcontent"> | <p class="pcontent"> | ||
− | + | <ol start="6"> | |
− | + | <li>Transform the product by heat shock.</li> | |
− | + | ||
</ol> | </ol> | ||
</p> | </p> | ||
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</br></br> | </br></br> | ||
<h3>Gel Dissociation</h3></br> | <h3>Gel Dissociation</h3></br> | ||
− | <p class="pcontent"> | + | <p class="pcontent"> |
− | + | <ol> | |
− | + | <li>Gel Extraction</li> | |
− | + | a. Excised the DNA fragment from the agarose gel.</br></br> | |
− | + | b. Transferred up to 300 mg of the gel slice to a 1.5 ml microcentrifuge tube.</br></br> | |
− | + | c. Added 500 μl of the Gel/PCR Bufffer to the sample and mixed by vortex. | |
− | + | Incubate | |
− | + | at 55~60℃ for 10 minutes (or until the gel slice has completely dissolved).</br></br> | |
+ | d. During the incubation, mixed by vortexing the tube every 2~3 minutes.</br></br> | ||
+ | e. Cooled the dissolved sample mixture to the room temperature.</br></br> | ||
</p> | </p> | ||
<h3>DNA Binding</h3></br> | <h3>DNA Binding</h3></br> |
Revision as of 10:32, 29 September 2018