Safety

There is a lot of dangers in a laboratory. So, everything cannot be done and there are rules to respect to protect the environment and ourselves. Due to an atmosphere of mistrust concerning GMOs in France, it is also very important to us to appease the opinion with a strict safety policy.

First, we will present here how the safety department in INSA is organized: what kind of structure received us, what is the French legislation concerning risks and how we have been formed. Then, we will detail what kind of risks we could met during the lab work and how we should avoid them with good laboratory habits. Finely, we will explain how we constructed our project to minimize the health and safety issues.

INSA Safety Department

During the summer, we worked in France in the laboratories of INSA with the help of the LISBP. We will present here how the safety department at INSA is organized, what is the French legislation concerning biological lab work and GMOs and how we have been formed on the risk prevention and the safety rules.

Organization of the INSA Safety Department

We have been received by the LISBP. The head of the laboratory where we worked is Carole Jouve and the organization of the safety department is described in the figure 1 below. We are very grateful for the welcoming of our colleagues and the time they spend to form us.

Legislation and French labor Law

We are working in a french engineering school (INSA Toulouse), thus we have to respect the french national regulations about working conditions and manipulation of GMOs.

As we work with microorganisms, we are concerned by the regulation on workers protection against risks resulting from their exposure to pathogenic biological agents (Decree No. 94-352 of 4 May 1994). It also includes human endoparasites which may cause infections, allergies or toxicity. This Decree is the French transposition of the Directive 90/679 / EEC and is also transcribed in the Labour Code (Articles L4421-1 R4421-1 to R4427-5). This Decree of the 16th July 2007 describes the technical preventive measures that must be followed in research laboratories, where workers are likely to be exposed to biological pathogens. We must obey to the rules of health, safety, and preventive medicine applied in public services in France (Decree No. 82-453). This decree refers to the Labour Code, Public Health Code and Environmental Code.

Formation received

To follow the legislation, we have been formed by Nathalie Doubrovine, the supervisor of the safety department of the LISBP. Her goal is to ensure both the well-being of the employees and the respect of safety rules and risk prevention. During the summer, we received different formations:

• General information: two formations have been done, one during a meeting with Nathalie Doubrovine and the other with the software NEO. General information has been presented about the chemical, biological and fire risks. We also learned the prevention of those risks and the reaction to have when one of those risks occurs. At the end of the formation, a little test was done to see if everyone understood everything. Also, before using any new device, a quick formation is done.

• Autoclave formation: our team has attended an autoclave training, which showed us the explosive and implosive dangers of the dispositive and the security measures to take to protect ourselves. A lab coat, heat resistant gloves and glasses were required for the manipulation of the autoclave. The standard protocols of loading and unloading it were also demonstrated.

• Ethidium bromide room: because of the oncogenic risk of the ethidium bromide, we have a specific room where we must work with this solution. Nothing can get outside the room when it comes inside and everyone in the room must wear protection gloves, a lab coat and protection goggles. UV are also used in this room and protection mask must be wore during its use.

Knowing the risks and minimize them

Hazards we could met

In the laboratory, there is a large variety of risks we could met. We will detail what risks could occurs and how the LISBP managed them here.

Main hazards :

External risks (earthquake, security issues…) :

LISBP is concerned by many different types of hazards due to a wide spectrum of scientific activities. One hazard category gathers risks that are not directly linked to the LISBP research activity such as the risks due to working conditions (noise, thermal atmosphere, etc.), electrical hazards, work on computer screens, falls, etc. The main hazard categories concern the substances handled for research purposes such as class 1 microorganisms (GMO or not), urban wastewaters, chemical products among including CMRs, cryogenic fluids, etc… A second main hazard category relates to equipment and pilot plants with specific risks such as equipment using pressurized liquids, gases or steam (autoclaves), instruments generating non-ionizing radiations (Laser, UV lights) and electromagnetic radiations (RMN).

Main hazards :

External risks (earthquake, security issues…) :