Based on our research, RiboJ first described as it is an insulator in genetic construct but there has no any data shown this insulation effected the downstream genetic parts. In 2018, there is a team have proved that RiboJ could increase the expression of downstream gene and there is the team member, Kalen P Clifton#1, Ethan M Jones#1, Sudip Paudel1, John P Marken2, Callan E Monette1, Andrew D Halleran2, Lidia Epp1, Margaret S Saha1*. Hence, our team, NCKU_Tainan have desided to enhance the expression and specificity of promoter gadA by adding a gene, RiboJ at the downstream of promoter gadA.

For the function determination, we have measured the fluorescent density of the plasmid with and without RiboJ in different pH environment. We incubated the bacteria in pH adjacent M9 medium and measure the fluorescent intensity (absorbance: 480nm, excitation: 510 nm) in a short period of time.

Based on our functional determination test results, the improvement has succeeded as RiboJ really could enhance the expression and specificity of downstream gene.