Difference between revisions of "Team:KUAS Korea/WetLab/Results"

 
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                 <h3>1. Beta-glucosidase expression</h3>
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                 <h4><strong>1. Beta-glucosidase expression</strong></h4>
  
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                 <p><br><br>Cooperator expresses beta-glucosidase with constitutive promoter to degrade cellobiose around it. We had the cell stock of cooperator with pCEL vector containing beta-glucosidase and transformed it into E.coli BW25113. The medium plate contains cellobiose instead of glucose to check whether E.coli that we transformed expresses beta-glucosidase on its surface. The streaking side below shows that cooperator grows on cellobiose containing M9 minimal medium.  
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                 <ol><br><br>Cooperator expresses beta-glucosidase with constitutive promoter to degrade cellobiose around it. We had the cell stock of cooperator with pCEL vector containing beta-glucosidase and transformed it into E.coli BW25113. The medium plate contains cellobiose instead of glucose to check whether E.coli that we transformed expresses beta-glucosidase on its surface. The streaking side below shows that cooperator grows on cellobiose containing M9 minimal medium.  
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<img src="https://static.igem.org/mediawiki/2018/0/0c/T--KUAS_Korea--BETAGlucosidase_expression.png" width="700px" height="500px" style="float: center;"hspace="" vspace="10">
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<br>[Fig 1.] Cultured cooperator on M9 minimal medium with 0.48% cellobiose
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                 <h3>2. GFP expression</h3>
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                <ol>Cheater expresses GFP when it obtains glucose. We had the cell stock of cheater with the vector encoding GFP as a reporter gene and transformed it firstly into E.coli DH5α and then to E.coli BW25113.
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                <p>Cheater expresses GFP when it obtains glucose. We had the cell stock of cheater with the vector encoding GFP as a reporter gene and transformed it firstly into E.coli DH5α and then to E.coli BW25113.
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<img src="https://static.igem.org/mediawiki/2018/4/48/T--KUAS_Korea--GFP_PART1.png" width="700px" height="500px" style="float: center;" hspace="5" vspace="100"
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<br>[Fig 2.] Cultured cheater on M9 minimal medium with 0.48% glucose
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<br>[Fig 2-1.] Cultured cheater under fluorescence microscopy showing the GFP activity
 
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Latest revision as of 08:56, 3 October 2018

Results



1. Beta-glucosidase expression



    Cooperator expresses beta-glucosidase with constitutive promoter to degrade cellobiose around it. We had the cell stock of cooperator with pCEL vector containing beta-glucosidase and transformed it into E.coli BW25113. The medium plate contains cellobiose instead of glucose to check whether E.coli that we transformed expresses beta-glucosidase on its surface. The streaking side below shows that cooperator grows on cellobiose containing M9 minimal medium.


[Fig 1.] Cultured cooperator on M9 minimal medium with 0.48% cellobiose



2. GFP expression

    Cheater expresses GFP when it obtains glucose. We had the cell stock of cheater with the vector encoding GFP as a reporter gene and transformed it firstly into E.coli DH5α and then to E.coli BW25113.


[Fig 2.] Cultured cheater on M9 minimal medium with 0.48% glucose






[Fig 2-1.] Cultured cheater under fluorescence microscopy showing the GFP activity






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