Difference between revisions of "Team:KUAS Korea/WetLab/Results"
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| + | <h4><strong>1. Beta-glucosidase expression</strong></h4> | ||
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| − | < | + | <ol><br><br>Cooperator expresses beta-glucosidase with constitutive promoter to degrade cellobiose around it. We had the cell stock of cooperator with pCEL vector containing beta-glucosidase and transformed it into E.coli BW25113. The medium plate contains cellobiose instead of glucose to check whether E.coli that we transformed expresses beta-glucosidase on its surface. The streaking side below shows that cooperator grows on cellobiose containing M9 minimal medium. |
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| − | < | + | <img src="https://static.igem.org/mediawiki/2018/0/0c/T--KUAS_Korea--BETAGlucosidase_expression.png" width="700px" height="500px" style="float: center;"hspace="" vspace="10"> |
| + | <br>[Fig 1.] Cultured cooperator on M9 minimal medium with 0.48% cellobiose | ||
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| − | < | + | <h4><strong>2. GFP expression</strong></h4> |
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| − | < | + | <ol>Cheater expresses GFP when it obtains glucose. We had the cell stock of cheater with the vector encoding GFP as a reporter gene and transformed it firstly into E.coli DH5α and then to E.coli BW25113. |
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| + | <img src="https://static.igem.org/mediawiki/2018/4/48/T--KUAS_Korea--GFP_PART1.png" width="700px" height="500px" style="float: center;" hspace="5" vspace="100" | ||
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| + | <br>[Fig 2.] Cultured cheater on M9 minimal medium with 0.48% glucose | ||
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| + | <img src="https://static.igem.org/mediawiki/2018/1/1b/T--KUAS_Korea--GFP_PART2.png" width="700px" height="500px" style="float: center;" hspace="5" vspace="100"> | ||
| + | <br>[Fig 2-1.] Cultured cheater under fluorescence microscopy showing the GFP activity | ||
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Latest revision as of 08:56, 3 October 2018
Results |
1. Beta-glucosidase expression
Cooperator expresses beta-glucosidase with constitutive promoter to degrade cellobiose around it. We had the cell stock of cooperator with pCEL vector containing beta-glucosidase and transformed it into E.coli BW25113. The medium plate contains cellobiose instead of glucose to check whether E.coli that we transformed expresses beta-glucosidase on its surface. The streaking side below shows that cooperator grows on cellobiose containing M9 minimal medium.
[Fig 1.] Cultured cooperator on M9 minimal medium with 0.48% cellobiose
2. GFP expression
- Cheater expresses GFP when it obtains glucose. We had the cell stock of cheater with the vector encoding GFP as a reporter gene and transformed it firstly into E.coli DH5α and then to E.coli BW25113.
[Fig 2.] Cultured cheater on M9 minimal medium with 0.48% glucose
[Fig 2-1.] Cultured cheater under fluorescence microscopy showing the GFP activity
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