1. Beta-glucosidase expression

Cooperator expresses beta-glucosidase with constitutive promoter to degrade cellobiose around it. We had the cell stock of cooperator with pCEL vector containing beta-glucosidase and transformed it into E.coli BW25113. The medium plate contains cellobiose instead of glucose to check whether E.coli that we transformed expresses beta-glucosidase on its surface. The streaking side below shows that cooperator grows on cellobiose containing M9 minimal medium.

[Fig 1.] Cultured cooperator on M9 minimal medium with 0.48% cellobiose

2. GFP expression

Cheater expresses GFP when it obtains glucose. We had the cell stock of cheater with the vector encoding GFP as a reporter gene and transformed it firstly into E.coli DH5α and then to E.coli BW25113.

[Fig 2.] Cultured cheater on M9 minimal medium with 0.48% glucose






[Fig 2-1.] Cultured cheater under fluorescence microscopy showing the GFP activity