|
|
Line 29: |
Line 29: |
| overflow: hidden; | | overflow: hidden; |
| padding-bottom: 150px; | | padding-bottom: 150px; |
− | background-color: #e5f1f7; | + | background-color: #ffffff; |
| } | | } |
| | | |
Line 231: |
Line 231: |
| <img class="cover" src="https://static.igem.org/mediawiki/2018/c/cf/T--NCTU_Formosa--Safety.png"> | | <img class="cover" src="https://static.igem.org/mediawiki/2018/c/cf/T--NCTU_Formosa--Safety.png"> |
| </div> | | </div> |
− | <div class="sec1" style="background-color:#e5f1f7;"> | + | <div class="sec1" style="background-color:#ffffff;"> |
| <div class="title"><p>Overview</p></div> | | <div class="title"><p>Overview</p></div> |
| <div class="text"> | | <div class="text"> |
Line 238: |
Line 238: |
| </div> | | </div> |
| </div> | | </div> |
− | <div class="sec2" style="background-color:#e5f1f7;"> | + | <div class="sec2" style="background-color:#ffffff;"> |
| <div class="title"><p>Safety Training and Laboratory</p></div> | | <div class="title"><p>Safety Training and Laboratory</p></div> |
| <div class="text"> | | <div class="text"> |
Line 244: |
Line 244: |
| </div> | | </div> |
| </div> | | </div> |
− | <div class="sec3" style="background-color:#e5f1f7;"> | + | <div class="sec3" style="background-color:#ffffff;"> |
| <div class="title"><p>Confirm the Safety of Bio-stimulator</p></div> | | <div class="title"><p>Confirm the Safety of Bio-stimulator</p></div> |
| <div class="title_3"><p>- High-temperature Sterilization Target on Modified <i>E.coli</i> ER2566 -</p></div> | | <div class="title_3"><p>- High-temperature Sterilization Target on Modified <i>E.coli</i> ER2566 -</p></div> |
Line 270: |
Line 270: |
| <div class="explanation"> | | <div class="explanation"> |
| <svg class="icon" aria-hidden="true" data-prefix="fas" data-icon="arrow-circle-up" class="svg-inline--fa fa-arrow-circle-up fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M8 256C8 119 119 8 256 8s248 111 248 248-111 248-248 248S8 393 8 256zm143.6 28.9l72.4-75.5V392c0 13.3 10.7 24 24 24h16c13.3 0 24-10.7 24-24V209.4l72.4 75.5c9.3 9.7 24.8 9.9 34.3.4l10.9-11c9.4-9.4 9.4-24.6 0-33.9L273 107.7c-9.4-9.4-24.6-9.4-33.9 0L106.3 240.4c-9.4 9.4-9.4 24.6 0 33.9l10.9 11c9.6 9.5 25.1 9.3 34.4-.4z"></path></svg> | | <svg class="icon" aria-hidden="true" data-prefix="fas" data-icon="arrow-circle-up" class="svg-inline--fa fa-arrow-circle-up fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M8 256C8 119 119 8 256 8s248 111 248 248-111 248-248 248S8 393 8 256zm143.6 28.9l72.4-75.5V392c0 13.3 10.7 24 24 24h16c13.3 0 24-10.7 24-24V209.4l72.4 75.5c9.3 9.7 24.8 9.9 34.3.4l10.9-11c9.4-9.4 9.4-24.6 0-33.9L273 107.7c-9.4-9.4-24.6-9.4-33.9 0L106.3 240.4c-9.4 9.4-9.4 24.6 0 33.9l10.9 11c9.6 9.5 25.1 9.3 34.4-.4z"></path></svg> |
− | Figure 3: LB Agar plate of boiling test to Lacticin+intein+CBD(33.9kDa) and Leucocyclicin Q | + | Figure 3: LB Agar plate of boiling test to Lacticin+intein+CBD(33.9kDa) and Leucocyclicin Q+intein+CBD(34.4kDa) |
| </div> | | </div> |
| <div class="text"> | | <div class="text"> |
− | <p>We also run SDS-PAGE to check the degradation of bacterioicins. The result shows that the peptide doesn’t degrade after boiling in 45 minutes.</p> | + | <p> We also run SDS-PAGE to check the degradation of bacterioicins. The result shows that the peptide doesn’t degrade after boiling in 45 minutes.</p> |
| </div> | | </div> |
− | <img src="https://static.igem.org/mediawiki/2018/7/71/T--NCTU_Formosa--Safety_96_B.png" class="sds"> | + | <img src="https://static.igem.org/mediawiki/2018/5/52/T--NCTU_Formosa--96_B_safetySDS.png" class="sds"> |
| <div class="explanation"> | | <div class="explanation"> |
| <svg class="icon" aria-hidden="true" data-prefix="fas" data-icon="arrow-circle-up" class="svg-inline--fa fa-arrow-circle-up fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M8 256C8 119 119 8 256 8s248 111 248 248-111 248-248 248S8 393 8 256zm143.6 28.9l72.4-75.5V392c0 13.3 10.7 24 24 24h16c13.3 0 24-10.7 24-24V209.4l72.4 75.5c9.3 9.7 24.8 9.9 34.3.4l10.9-11c9.4-9.4 9.4-24.6 0-33.9L273 107.7c-9.4-9.4-24.6-9.4-33.9 0L106.3 240.4c-9.4 9.4-9.4 24.6 0 33.9l10.9 11c9.6 9.5 25.1 9.3 34.4-.4z"></path></svg> | | <svg class="icon" aria-hidden="true" data-prefix="fas" data-icon="arrow-circle-up" class="svg-inline--fa fa-arrow-circle-up fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M8 256C8 119 119 8 256 8s248 111 248 248-111 248-248 248S8 393 8 256zm143.6 28.9l72.4-75.5V392c0 13.3 10.7 24 24 24h16c13.3 0 24-10.7 24-24V209.4l72.4 75.5c9.3 9.7 24.8 9.9 34.3.4l10.9-11c9.4-9.4 9.4-24.6 0-33.9L273 107.7c-9.4-9.4-24.6-9.4-33.9 0L106.3 240.4c-9.4 9.4-9.4 24.6 0 33.9l10.9 11c9.6 9.5 25.1 9.3 34.4-.4z"></path></svg> |
− | Figure 4: SDS-PAGE gel and the concentrations of boiling test to bacteriocins.<p> (A) Enterocin 96+intein+CBD(35.9kDa) (B) Enteroicin B+intein+CBD(35.5kDa)</p> | + | Figure 4: SDS-PAGE for Enterocin 96 and Enteroicin B.<p><br> (A) Boil for 0 minute. (B) Boil for 15 minutes. (C) Boil for 30 minutes. (D) Boil for 45 minutes.</p> |
| </div> | | </div> |
− | <img src="https://static.igem.org/mediawiki/2018/1/10/T--NCTU_Formosa--Safety_Bov_Dur.png" class="sds"> | + | <img src="https://static.igem.org/mediawiki/2018/2/2a/T--NCTU_Formosa--Bov_Dur_safetySDS.png" class="sds"> |
| <div class="explanation"> | | <div class="explanation"> |
| <svg class="icon" aria-hidden="true" data-prefix="fas" data-icon="arrow-circle-up" class="svg-inline--fa fa-arrow-circle-up fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M8 256C8 119 119 8 256 8s248 111 248 248-111 248-248 248S8 393 8 256zm143.6 28.9l72.4-75.5V392c0 13.3 10.7 24 24 24h16c13.3 0 24-10.7 24-24V209.4l72.4 75.5c9.3 9.7 24.8 9.9 34.3.4l10.9-11c9.4-9.4 9.4-24.6 0-33.9L273 107.7c-9.4-9.4-24.6-9.4-33.9 0L106.3 240.4c-9.4 9.4-9.4 24.6 0 33.9l10.9 11c9.6 9.5 25.1 9.3 34.4-.4z"></path></svg> | | <svg class="icon" aria-hidden="true" data-prefix="fas" data-icon="arrow-circle-up" class="svg-inline--fa fa-arrow-circle-up fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M8 256C8 119 119 8 256 8s248 111 248 248-111 248-248 248S8 393 8 256zm143.6 28.9l72.4-75.5V392c0 13.3 10.7 24 24 24h16c13.3 0 24-10.7 24-24V209.4l72.4 75.5c9.3 9.7 24.8 9.9 34.3.4l10.9-11c9.4-9.4 9.4-24.6 0-33.9L273 107.7c-9.4-9.4-24.6-9.4-33.9 0L106.3 240.4c-9.4 9.4-9.4 24.6 0 33.9l10.9 11c9.6 9.5 25.1 9.3 34.4-.4z"></path></svg> |
− | Figure 5: SDS-PAGE gel and the concentrations of boiling test to bacteriocins.<p>(C)Bovicin HJ50+intein +CBD(34.25kDa) (D) Durancin +intein+CBD(35.3kDa)</p> | + | Figure 5: SDS-PAGE for Bovicin HJ50 and Durancin TW-49M.<p><br> (A) Boil for 0 minute. (B) Boil for 15 minutes. (C) Boil for 30 minutes. (D) Boil for 45 minutes.</p> |
| </div> | | </div> |
| <img src="https://static.igem.org/mediawiki/2018/4/42/T--NCTU_Formosa--Safety_Lac.png" class="sds"> | | <img src="https://static.igem.org/mediawiki/2018/4/42/T--NCTU_Formosa--Safety_Lac.png" class="sds"> |
Line 304: |
Line 304: |
| </html> | | </html> |
| | | |
− | {{NCTU_Formosa/Top_button}}
| |
| {{NCTU_Formosa/Footer}} | | {{NCTU_Formosa/Footer}} |