Difference between revisions of "Team:NCTU Formosa/Composite Part"

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   <title>Basic Parts</title>
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   <title>Composite Parts</title>
  
 
   <script src="jQueryAssets/jquery-1.11.1.min.js"></script>
 
   <script src="jQueryAssets/jquery-1.11.1.min.js"></script>
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     </div>
 
     </div>
 
     <div class="sec1">
 
     <div class="sec1">
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       <img src="https://static.igem.org/mediawiki/2018/a/a5/T--NCTU_Formosa--Basic_parts.png" class="basic_title">
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       <img src="https://static.igem.org/mediawiki/2018/f/f8/T--NCTU_Formosa--Composite_parts.png" class="basic_title">
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       <img src="https://static.igem.org/mediawiki/2018/9/95/T--NCTU_Formosa--Parts_dec1.png" class="dec_1">
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       <img src="https://static.igem.org/mediawiki/2018/5/5d/T--NCTU_Formosa--Parts_dec3.png" class="dec_1">
 
       <div class="title_1"><p>Overview</p></div>
 
       <div class="title_1"><p>Overview</p></div>
 
       <div class="text">
 
       <div class="text">
 
         <p>
 
         <p>
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           &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;This year we decided to create eight basic parts. These parts contain the single sequences of each antimicrobial peptide. They all show the inhibition activity against <i>Bacillus subtilis</i>, which is one of the example of our system. We listed all the basic parts we constructed below.
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           &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We tried to express the eight antimicrobial peptides in <i>E. coli<i/>. These composite parts contains the coding sequence, RBS, a T7 promoter, and also intein and CBD sequences for better purification.<br><br>
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit <i>Bacillus subtilis</i>.
 
         </p>
 
         </p>
 
       </div>
 
       </div>
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       <div class="basic_parts">
 
       <div class="basic_parts">
 
         <div class="title_1"><p>Bacteriocins</p></div>
 
         <div class="title_1"><p>Bacteriocins</p></div>
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         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599000" class="name" target="_blank" style="text-decoration: none;"><p>Subtilosin(BBa_K2599000)</p></a>
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         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599008" class="name" target="_blank" style="text-decoration: none;"><p>Subtilosin-Intein-CBD(BBa_K2599008)</p></a>
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         <img src="https://static.igem.org/mediawiki/2018/2/2c/T--NCTU_Formosa--Sub.png" class="bac">
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         <img src="https://static.igem.org/mediawiki/2018/b/bf/T--NCTU_Formosa--Sub_2.png" class="bac">
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         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599001" class="name" target="_blank" style="text-decoration: none;"><p>Bovicin HJ50(BBa_K2599001)</p></a>
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         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599009" class="name" target="_blank" style="text-decoration: none;"><p>Bovicin HJ50-Intein-CBD(BBa_K2599009)</p></a>
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         <img src="https://static.igem.org/mediawiki/2018/f/f2/T--NCTU_Formosa--Bov.png" class="bac">
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         <img src="https://static.igem.org/mediawiki/2018/f/f5/T--NCTU_Formosa--Bov_2.png" class="bac">
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         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599002" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin A(BBa_K2599002)</p></a>
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         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599010" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin A-Intein-CBD(BBa_K2599010)</p></a>
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         <img src="https://static.igem.org/mediawiki/2018/e/e5/T--NCTU_Formosa--Ent_A.png" class="bac">
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         <img src="https://static.igem.org/mediawiki/2018/4/45/T--NCTU_Formosa--Ent_A_2.png" class="bac">
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         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599003" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin B(BBa_K2599003)</p></a>
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         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599011" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin B-Intein-CBD(BBa_K2599011)</p></a>
−
         <img src="https://static.igem.org/mediawiki/2018/4/4a/T--NCTU_Formosa--Ent_B.png" class="bac">
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         <img src="https://static.igem.org/mediawiki/2018/5/5f/T--NCTU_Formosa--Ent_B_2.png" class="bac">
−
         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599004" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin 96(BBa_K2599004)</p></a>
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         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599012" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin 96-Intein-CBD(BBa_K2599012)</p></a>
−
         <img src="https://static.igem.org/mediawiki/2018/3/35/T--NCTU_Formosa--Ent_96.png" class="bac">
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         <img src="https://static.igem.org/mediawiki/2018/d/da/T--NCTU_Formosa--Ent_96_2.png" class="bac">
−
         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599005" class="name" target="_blank" style="text-decoration: none;"><p>Lacticin Z(BBa_K2599005)</p></a>
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         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599013" class="name" target="_blank" style="text-decoration: none;"><p>Lacticin Z-Intein-CBD(BBa_K2599013)</p></a>
−
         <img src="https://static.igem.org/mediawiki/2018/d/de/T--NCTU_Formosa--Lac.png" class="bac">
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         <img src="https://static.igem.org/mediawiki/2018/8/8b/T--NCTU_Formosa--Lac_2.png" class="bac">
−
         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599006" class="name" target="_blank" style="text-decoration: none;"><p>Leucocyclicin Q(BBa_K2599006)</p></a>
+
         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599014" class="name" target="_blank" style="text-decoration: none;"><p>Leucocyclicin Q-Intein-CBD(BBa_K2599014)</p></a>
−
         <img src="https://static.igem.org/mediawiki/2018/d/d5/T--NCTU_Formosa--Leu.png" class="bac">
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         <img src="https://static.igem.org/mediawiki/2018/1/13/T--NCTU_Formosa--Leu_2.png" class="bac">
−
         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599007" class="name" target="_blank" style="text-decoration: none;"><p>Durancin TW-49M(BBa_K2599007)</p></a>
+
         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599015" class="name" target="_blank" style="text-decoration: none;"><p>Durancin TW-49M-Intein-CBD(BBa_K2599015)</p></a>
−
         <img src="https://static.igem.org/mediawiki/2018/a/a3/T--NCTU_Formosa--Dur.png" class="bac" style="margin-bottom: 50px;" >
+
         <img src="https://static.igem.org/mediawiki/2018/b/b4/T--NCTU_Formosa--Dur_2.png" class="bac" style="margin-bottom: 50px;" >
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 +
        <div class="title_1"><p>Improvement</p></div>
 
       </div>
 
       </div>
  
 
       <img src="https://static.igem.org/mediawiki/2018/9/99/T--NCTU_Formosa--Parts_button.png" class="part_button" id="pb" onmouseup="mouseUp()">
 
       <img src="https://static.igem.org/mediawiki/2018/9/99/T--NCTU_Formosa--Parts_button.png" class="part_button" id="pb" onmouseup="mouseUp()">
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       <img src="https://static.igem.org/mediawiki/2018/a/ad/T--NCTU_Formosa--Parts_dec2.png" class="dec_2">
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       <img src="https://static.igem.org/mediawiki/2018/6/61/T--NCTU_Formosa--Parts_dec4.png" class="dec_2">
  
 
     </div>
 
     </div>

Revision as of 13:46, 8 October 2018

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Navigation Bar Composite Parts

Overview

     We tried to express the eight antimicrobial peptides in E. coli. These composite parts contains the coding sequence, RBS, a T7 promoter, and also intein and CBD sequences for better purification.

     The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit Bacillus subtilis.

Bacteriocins

Subtilosin-Intein-CBD(BBa_K2599008)

Bovicin HJ50-Intein-CBD(BBa_K2599009)

Enterocin A-Intein-CBD(BBa_K2599010)

Enterocin B-Intein-CBD(BBa_K2599011)

Enterocin 96-Intein-CBD(BBa_K2599012)

Lacticin Z-Intein-CBD(BBa_K2599013)

Leucocyclicin Q-Intein-CBD(BBa_K2599014)

Durancin TW-49M-Intein-CBD(BBa_K2599015)

Improvement