We chose E. coli as our chassis organism. Three lab strains were selected: “DH5 alpha”, “BL21(DE3)”, “W3110”, and ”W3110 (L5T7)”. These lab strains are reported to be not pathogenic to human. We worked these strains in authorized labs (biosafety level 1 lab and higher) only.

Three new basic parts with open reading frame were created in our project. They are Rubisco, prk, and CA. Three genes are derived from Synechococcus elongatus pcc. 7002 and Synechococcus elongatus pcc. 7942. Synechococcus elongatus is a biosafety level 1 nonpathogenic cyanobacteria.

This is the third year since the establishment of iGEM NCKU-Tainan team. We have raised the awareness of synthetic biology in our campus. Mrs. Jenny Su and the College of Engineering decided to give us a headquarter which contains a meeting room and a lab. To meet the lab standard of CDC (Center for Disease Control), we made contact with Mrs. Liu Yee Fen, the contact person of Center for Occupational safety and Health and Environmental Protection, which is the authorized unit in our campus responsible for lab safety and security. With the assistance of our instructor Dr. Ng, we successfully certify our lab to BSL1 saftey level one lab. Mrs. Liu gave us some lab security instructions and advice. She also suggested that each of our members should attend the biosafety lab training program.

Expect the lab in iGEM headquarter, wet team has constructed out design in various labs whose supervisors are Dr. I-Son Ng, Dr. I-Hsiu Huang, Dr. Han-Ching Wang, and Dr. Hashimoto Masayuki. The labs mentioned above are all certified biosafety level one.

It is a must for anyone to pass the authorized training section before conducting any experiments in lab. Since our applied design must be tested with constructed bacteria, each of our team members will have chances to approach the lab. We decided that whole team should be certified to do the experiments in biosafety level one lab. Team members from biotechnology have already passed the training session, and the rest of team members have passed the training online course. We believe that learning safety and security knowledge lower the risk when we conducted experiments inside biolab.

The goal of our project is to fix CO2 emitted from industries by using modified E.coli and produce high value compound. At the same time, we aware of the safety concerns of introducing synthetic organisms into the environment. We designed a bioreactor that ensures the engineered E.coli act as a closed system and separated from the environment. Each inlet and outlet of the bioreactor is equipped with bacteria proof filter too. Furthermore, the collected residues will be sterilized in sterilization equipment.

The goal of our project is to fix CO2 emitted from industries by using modified E.coli and produce high value compound. At the same time, we aware of the safety concerns of introducing synthetic organisms into the environment. We designed a bioreactor that ensures the engineered E.coli act as a closed system and separated from the environment. Each inlet and outlet of the bioreactor is equipped with filter too which prevents the outflow of the engineered bacteria. We tested the safety of our device by incubating bacteria inside. We collected the waste sample from the filter and apply it on to the agar plate. Comparing with the control group that does not passed the filter, we found out that the filter can effectively isolate the bacteria inside our device.

In our applied design, we planned to extract protein and bioproduct from E. coli . The waste should be then collected and stertilized. We planned to combine the waste heat recovery system in the factory to stertilize the waste medium . One of the main goal of our applied design, to turn waste to valuable resource, is fulfilled in this biosafety design.