Difference between revisions of "Team:NCKU Tainan/Protocol"

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                                                                 kanamycin, 1/4000 chloramphenicol to log phase(about 3 hr).</li>
 
                                                                 kanamycin, 1/4000 chloramphenicol to log phase(about 3 hr).</li>
 
                                                             <li class="licontent">Centrifuge them in 22℃ ,3200xg for 5 minutes , then refresh by the M9 medium with 4%xylose and 0.1%LB.</li>
 
                                                             <li class="licontent">Centrifuge them in 22℃ ,3200xg for 5 minutes , then refresh by the M9 medium with 4%xylose and 0.1%LB.</li>
                                                             <li class="licontent">Culture the bacteria for 24 hr in both O<sub>2</sub> and 5%CO<sub>2</sub> incubator, and test the O.D. value at every 6 hours.</li>
+
                                                             <li class="licontent">Culture the bacteria for 24 hr in both O<sub>2</sub> and 5%CO<sub>2</sub> incubator, and test the O.D.600 value at every 6 hours. Measure the xylose concentration on 12th hour and 24th hour using DNS reducing sugar kit.</li>
 
                                                         </ol>
 
                                                         </ol>
 
                                                     </div>
 
                                                     </div>

Revision as of 08:26, 12 October 2018

Protocol

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