Oscarliu117 (Talk | contribs) |
Oscarliu117 (Talk | contribs) |
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<div id="pt"> | <div id="pt"> | ||
− | <h8>PRK</h8> | + | <h8>PRK</h8></br> |
<p class="pcontent"> | <p class="pcontent"> | ||
Achievements:</br> | Achievements:</br> | ||
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into | into | ||
different plasmid</li> | different plasmid</li> | ||
− | </ol> | + | </ol></br> |
<p class="pcontent"> | <p class="pcontent"> | ||
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37.7kDa. | 37.7kDa. | ||
</p> | </p> | ||
− | </div> | + | </div></br> |
<div id="pt"> | <div id="pt"> | ||
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native pathway and be converted into RuBP. Growth arrest of PRK strain should | native pathway and be converted into RuBP. Growth arrest of PRK strain should | ||
be observed. | be observed. | ||
− | </p> | + | </p></br> |
<p class="pcontent"> | <p class="pcontent"> | ||
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merely | merely | ||
no growth, which proves the function of PRK. | no growth, which proves the function of PRK. | ||
− | </p> | + | </p></br> |
<p class="pcontent"> | <p class="pcontent"> | ||
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little bit of growth retard compare to non-PRK expressing strain. However, the | little bit of growth retard compare to non-PRK expressing strain. However, the | ||
toxicity is much less than high copy number expressing strain. | toxicity is much less than high copy number expressing strain. | ||
− | </p> | + | </p></br> |
<p class="pcontent"> | <p class="pcontent"> | ||
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<div id="pt"> | <div id="pt"> | ||
− | <h8>CA</h8> | + | <h8>CA</h8></br> |
<p class="pcontent"> | <p class="pcontent"> | ||
Achievements:</br> | Achievements:</br> | ||
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<li>Measure the activity of CA enzyme</li> | <li>Measure the activity of CA enzyme</li> | ||
− | </ol> | + | </ol></br> |
<p class="pcontent"> | <p class="pcontent"> | ||
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Fig. 6 Confirmation of CA expression in BL21(DE3). The expected protein size is | Fig. 6 Confirmation of CA expression in BL21(DE3). The expected protein size is | ||
27.9kDa. | 27.9kDa. | ||
− | </p> | + | </p></br> |
</div> | </div> | ||
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solution which will be described in the following content. | solution which will be described in the following content. | ||
</p> | </p> | ||
− | </div> | + | </div></br> |
<div id="pt"> | <div id="pt"> | ||
− | <h8>Rubisco</h8> | + | <h8>Rubisco</h8></br> |
<p class="pcontent"> | <p class="pcontent"> | ||
Achievements:</br> | Achievements:</br> | ||
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<li>The SDS-PAGE of PRK showed that the expression of PRK in the expected size</li> | <li>The SDS-PAGE of PRK showed that the expression of PRK in the expected size</li> | ||
− | </ol> | + | </ol></br> |
<p class="pcontent"> | <p class="pcontent"> | ||
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<div id="pt"> | <div id="pt"> | ||
− | <h8>Rubisco</h8> | + | <h8>Rubisco</h8></br> |
<p class="pcontent"> | <p class="pcontent"> | ||
Achievements:</br> | Achievements:</br> | ||
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<li>Compare different CO2 incubation environment</li> | <li>Compare different CO2 incubation environment</li> | ||
− | </ol> | + | </ol></br> |
− | <h8>Overview</h8> | + | <h8>Overview</h8></br> |
<p class="pcontent"> | <p class="pcontent"> | ||
In the total solution experiment, we strive to measure the carbon fixation | In the total solution experiment, we strive to measure the carbon fixation | ||
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O.D. 600 and xylose consumption. We have measure various construction to prove | O.D. 600 and xylose consumption. We have measure various construction to prove | ||
that the enzyme of our construction is necessary for carbon fixation. | that the enzyme of our construction is necessary for carbon fixation. | ||
− | </p> | + | </p></br> |
− | <p class=" | + | <p class="pcontent"> |
The test samples below were incubated in an altered M9 medium which substitute | The test samples below were incubated in an altered M9 medium which substitute | ||
glucose to xylose. 1/1000 of LB medium was added to support some rare elements. | glucose to xylose. 1/1000 of LB medium was added to support some rare elements. | ||
Since the concentration of LB medium is too low, it doesn’t contribute the | Since the concentration of LB medium is too low, it doesn’t contribute the | ||
carbon source of the bacteria. | carbon source of the bacteria. | ||
− | </p> | + | </p></br> |
<p class="pcontent"> | <p class="pcontent"> | ||
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carbon fixation. We can compare this index of each strain to find out the | carbon fixation. We can compare this index of each strain to find out the | ||
strain that has highest capacity of carbon fixing. | strain that has highest capacity of carbon fixing. | ||
− | </p> | + | </p></br> |
<p class="pcontent"> | <p class="pcontent"> | ||
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medium. | medium. | ||
A linear relationship between O.D. and dry cell weight is observed. | A linear relationship between O.D. and dry cell weight is observed. | ||
− | </p> | + | </p></br> |
<ol start="2"> | <ol start="2"> | ||
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growth condition, we assume that such error can be ignore during the | growth condition, we assume that such error can be ignore during the | ||
following calculation.</li> | following calculation.</li> | ||
− | </ol> | + | </ol></br> |
<p class="pcontent"> | <p class="pcontent"> |
Revision as of 14:00, 12 October 2018
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