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Huiyee11186 (Talk | contribs) |
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<li>Compare the carbon fixation rate of W3110 and BL21(DE3) E. coli strains</li> | <li>Compare the carbon fixation rate of W3110 and BL21(DE3) E. coli strains</li> | ||
− | <li>Compare different | + | <li>Compare different CO<sub>2</sub> incubation environment</li> |
</ol></br> | </ol></br> | ||
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provided in our medium) as its carbon source. Although some native E. coli | provided in our medium) as its carbon source. Although some native E. coli | ||
pathway | pathway | ||
− | may utilize | + | may utilize CO<sub>2</sub> (such as lipid synthesis), the amount is too small to consider. |
As | As | ||
for engineered strain, carbon dioxide can be utilized as it’s carbon source. By | for engineered strain, carbon dioxide can be utilized as it’s carbon source. By | ||
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strain and discovered that control strain without IPTG induction produce less | strain and discovered that control strain without IPTG induction produce less | ||
rubisco enzyme than the experiment. Without rubisco, the bypass pathway is not | rubisco enzyme than the experiment. Without rubisco, the bypass pathway is not | ||
− | capable of using | + | capable of using CO<sub>2</sub>. We found out that the strain without Rubisco has higher |
XUI, symbolizing that rubisco is essential in carbon fixation pathway. | XUI, symbolizing that rubisco is essential in carbon fixation pathway. | ||
</p> | </p> | ||
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<p class="pcontent"> | <p class="pcontent"> | ||
− | Fig. 13 Shows the growth and XUI measured in 5% | + | Fig. 13 Shows the growth and XUI measured in 5% CO<sub>2</sub> incubation of 12 hours |
respectively. Lower growth of the strain that contains. The XUI of the strain | respectively. Lower growth of the strain that contains. The XUI of the strain | ||
that contains both Rubisco and PRK shows statistically significant decrease | that contains both Rubisco and PRK shows statistically significant decrease | ||
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XUI of this strain and compare with the previous strain that only contains PRK | XUI of this strain and compare with the previous strain that only contains PRK | ||
and Rubisco. We found out that CA can raise the growth and lower the XUI. We | and Rubisco. We found out that CA can raise the growth and lower the XUI. We | ||
− | infer that CA can enhance the intracellular | + | infer that CA can enhance the intracellular CO<sub>2</sub> concentration and thus increase |
the carbon flux of the bypass pathway. The efficiency of the bypass pathway is | the carbon flux of the bypass pathway. The efficiency of the bypass pathway is | ||
thus been increased. | thus been increased. | ||
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<p class="pcontent"> | <p class="pcontent"> | ||
Fig. 14 Shows the growth and XUI comparison of each strains. All the tested | Fig. 14 Shows the growth and XUI comparison of each strains. All the tested | ||
− | strains are incubated in 5% | + | strains are incubated in 5% CO<sub>2</sub> incubator for 12 hr. 0.1mM of IPTG was added to |
induce the protein expression. We can observe that growth speed of the | induce the protein expression. We can observe that growth speed of the | ||
construction has been increased with the CA, and the XUI of the strain that | construction has been increased with the CA, and the XUI of the strain that |
Revision as of 20:00, 13 October 2018
Results