Difference between revisions of "Team:ICT-Mumbai/Composite Part"

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<p>The aim of our project is to isolate the promoters of the genes that are upregulated in presence of root exudates of the crops and use these promoters in different applications. As a case study, we worked on construction of a positive feedback circuit to amplify the production of PhoD (alkaline phosphatase) in response to root exudates, during which we prepared several composite parts and intermediates and submitted them to registry.</p>
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  <li>BBa_2802000:</li>
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<p>This part is a composite part composed of BBa_K274380 and BBa_I746351. A promoter can be cloned upstream of this part, and the PSP3 <I>pag</I> phage activator that will be produced will bind to the PF phage promoter to drive expression of the PSP3 <I>pag</I> phage activator, forming a genetic amplifier. </p>
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  <li>BBa_2802001:</li>
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<p>This part is a composite part composed of BBa_K274380, BBa_I746351 and BBa_K518012. A promoter can be cloned upstream of this part, and the PSP3 <I>pag</I> phage activator that will be produced will bind to the PFF phage promoter to drive expression of the PSP3 <I>pag</I> phage activator, forming a genetic amplifier. <I>rfp</I>, which will be produced from the gene cloned downstream of the second <I>pag</I> gene, will act as a detectable output of this genetic amplifier.
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  <li>BBa_2802002:</li>
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<p>This part is a composite part composed of BBa_K274371 and BBa_I746350. A promoter can be cloned upstream of this part, and the P2 <I>ogr</I> phage activator that will be produced will bind to the PO phage promoter to drive expression of the P2 <I>ogr</I> phage activator, forming a genetic amplifier.</p>
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<li>BBa_2802003:</li>
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<p>This part is a composite part composed of BBa_K274371, BBa_I746350 and BBa_K518012. A promoter can be cloned upstream of this part, and the P2 <I>ogr</I> phage activator that will be produced will bind to the PO phage promoter to drive expression of the P2 <I>ogr</I> phage activator, forming a genetic amplifier. RFP, which will be produced from the gene cloned downstream of the second <I>ogr</I> gene, will act as a detectable output of this genetic amplifier.</p>
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<center><groupparts>iGEM18 ICT-Mumbai</groupparts></center>
  
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Composite Parts</h1>
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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Following are the intermediate parts submitted by the team to the iGEM registry</p>
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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<th>Type</th>
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<th>Description</th>
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<th>Designer</th>
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<h3>Note</h3>
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
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<h3>Best Composite Part Special Prize</h3>
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<p>To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2018.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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Revision as of 08:14, 14 October 2018

Simply




Contact us: igemteamictmumbai@gmail.com  | Follow us:

Parts

The aim of our project is to isolate the promoters of the genes that are upregulated in presence of root exudates of the crops and use these promoters in different applications. As a case study, we worked on construction of a positive feedback circuit to amplify the production of PhoD (alkaline phosphatase) in response to root exudates, during which we prepared several composite parts and intermediates and submitted them to registry.


  1. BBa_2802000:

    2. This part is a composite part composed of BBa_K274380 and BBa_I746351. A promoter can be cloned upstream of this part, and the PSP3 pag phage activator that will be produced will bind to the PF phage promoter to drive expression of the PSP3 pag phage activator, forming a genetic amplifier.

  2. BBa_2802001:

    3. This part is a composite part composed of BBa_K274380, BBa_I746351 and BBa_K518012. A promoter can be cloned upstream of this part, and the PSP3 pag phage activator that will be produced will bind to the PFF phage promoter to drive expression of the PSP3 pag phage activator, forming a genetic amplifier. rfp, which will be produced from the gene cloned downstream of the second pag gene, will act as a detectable output of this genetic amplifier.

  3. BBa_2802002:

    4. This part is a composite part composed of BBa_K274371 and BBa_I746350. A promoter can be cloned upstream of this part, and the P2 ogr phage activator that will be produced will bind to the PO phage promoter to drive expression of the P2 ogr phage activator, forming a genetic amplifier.

  4. BBa_2802003:

    5. This part is a composite part composed of BBa_K274371, BBa_I746350 and BBa_K518012. A promoter can be cloned upstream of this part, and the P2 ogr phage activator that will be produced will bind to the PO phage promoter to drive expression of the P2 ogr phage activator, forming a genetic amplifier. RFP, which will be produced from the gene cloned downstream of the second ogr gene, will act as a detectable output of this genetic amplifier.

<groupparts>iGEM18 ICT-Mumbai</groupparts>


Following are the intermediate parts submitted by the team to the iGEM registry

Name Type Description Designer