Difference between revisions of "Team:NCKU Tainan/Design"

Line 86: Line 86:
 
                                         3PGA will then be converted to pyruvate by the native metabolic system of <i>E. coli</i>.  
 
                                         3PGA will then be converted to pyruvate by the native metabolic system of <i>E. coli</i>.  
 
                                         After mining information from various publications,  
 
                                         After mining information from various publications,  
                                         we selected RuBisCO from <i>Synechococcus elongatus</i> PCC. 7002, which is a well-studied cyanobacteria.  
+
                                         we selected RuBisCO from <i>Synechococcus elongatus</i> PCC7002, which is a well-studied cyanobacteria.  
 
                                         Its genome is completely sequenced and it is often used as a model organism for gene manipulation.  
 
                                         Its genome is completely sequenced and it is often used as a model organism for gene manipulation.  
 
                                         Previous research has utilized <i>E. coli</i> as a host of random mutagenesis to enhance the activity of <i>Synechococcus</i> RuBisCO.
 
                                         Previous research has utilized <i>E. coli</i> as a host of random mutagenesis to enhance the activity of <i>Synechococcus</i> RuBisCO.
Line 118: Line 118:
 
                                         In cyanobacteria, Rubisco and carbonic anhydrase (CA) is encapsulated in a microcompartment, the carboxysome.  
 
                                         In cyanobacteria, Rubisco and carbonic anhydrase (CA) is encapsulated in a microcompartment, the carboxysome.  
 
                                         Carbonic anhydrase, also known as carbonate dehydratase, is involved in the interconversion between CO<sub>2</sub> and HCO<sub>3</sub><sup>-</sup>. This enzyme can be found in most organisms, including <i>E. coli</i> but the difference is its catalyzing rate in hydration and dehydration of CO2. Therefore,  
 
                                         Carbonic anhydrase, also known as carbonate dehydratase, is involved in the interconversion between CO<sub>2</sub> and HCO<sub>3</sub><sup>-</sup>. This enzyme can be found in most organisms, including <i>E. coli</i> but the difference is its catalyzing rate in hydration and dehydration of CO2. Therefore,  
                                         we will incorporate into our system the carbonic anhydrase gene from <i>Synechococcus elongatus</i> PCC. 7002.
+
                                         we will incorporate into our system the carbonic anhydrase gene from <i>Synechococcus elongatus</i> PCC7002.
 
                                     </p>
 
                                     </p>
 
                                     <img class="gif" src="https://static.igem.org/mediawiki/2018/3/34/T--NCKU_Tainan--design_CA.gif" alt="Rubisco">
 
                                     <img class="gif" src="https://static.igem.org/mediawiki/2018/3/34/T--NCKU_Tainan--design_CA.gif" alt="Rubisco">

Revision as of 12:19, 14 October 2018

Design

Follow us

Contact us

igem.ncku.tainan@gmail.com
No.1, Daxue Rd., East Dist., Tainan City 701, Taiwan (R.O.C.)