Difference between revisions of "Team:NEU China B/Improve"

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<h3>★  ALERT! </h3>
 
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
 
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
 
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<h1>Improve</h1>
 
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<p>For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Registry. Please include a link to your improved part on this page.</p>
 
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<h3>Gold Medal Criterion #2</h3>
 
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<p><b>Standard Tracks:</b> Create a new part that has a functional improvement upon an existing BioBrick part. The sequences of the new and existing parts must be different. You must perform experiments with both parts to demonstrate this improvement.  Document the experimental characterization on the Part's Main Page on the Registry for both the existing and new parts. Both the new and existing Main Page of each Part’s Registry entry must reference each other. Submit a sample of the new part to the Registry.
 
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The existing part must NOT be from your 2018 part number range and must be different from the part documented in bronze #4.
 
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<b>Special Tracks:</b> Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.</p>
 
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<H2>
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BBa_k2824008:Lldr-T7-lldPRD operon promoter-GFP
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</H2>
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<p>
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<br>The role of this part is to try to control the concentration of lactic acid on the LLDPRD promoter under the
 +
control of quorum sensingg. In the case of LLdr gene expression, the subsequent gene expression was initiated.
 +
<br>This part also is constructed based on the part lldRO1-plldR-lldRO2 (BBa_k82000) . lldRO1-plldR-lldRO2 part is a
 +
lactic acid regulated promoter that is open to gene transcription in the presence of lactic acid. Comparing with the
 +
T7-lldPRD operon promoter-GFP part, the improvement of this part is that the LLDR is added to part and placed under
 +
the control of two promoters respectively to form a double expression regulatory system. This comparison with the two
 +
plasmids working together is more convenient for testing the success of the system.
 +
<br>We can easily find that the Lldr-T7-lldPRD operon promoter-GFP part are in a higher level of expression comparing
 +
to the lldPRD operon promoter-GFP when the lactate concentration is lower than nearly 1.5m mol/L. Considering that
 +
the lactate concentration in yogurt is generally no more than 1m mol/L, the conclusion can given that the T7 promoter
 +
can enhance the signal intensity for our experiments.
 +
</p>
  
 +
<h2>
 +
BBa_k2824006:T7-lldPRD operon promoter-GFP
 +
</h2>
 +
<p>
 +
<br>LLDPRD can sense the presence of lactic acid, in the absence of lactic acid, only a lower background level of
 +
expression, but in the presence of lactic acid can open expression, gene transcription. We added the GFP gene into
 +
the gene and used green fluorescence as a marker to recognize the presence of lactic acid. In addition, as a strong
 +
promoter, T7 can improve the expression level of the target gene.
 +
<img src="https://static.igem.org/mediawiki/2018/e/ea/T--NEU_China_B--ct8.png">
 +
<img src="https://static.igem.org/mediawiki/2018/3/34/T--NEU_China_B--improve2.png">
 +
<br>This part is constructed based on the part lldRO1-plldR-lldRO2 (BBa_k82000) . lldRO1-plldR-lldRO2 part is a
 +
lactic acid regulated promoter that is open to gene transcription in the presence of lactic acid. We added a sequence
 +
of T 7 promoter and GFP fluorescent protein gene before and after this part Separately. It can effectively improve
 +
the expression of the target gene and detect whether the target gene is expressed or not. At the same time, it can
 +
accurately determine the amount of the target gene. This allows the overall work of our system to be reflected in
 +
digital form.
 +
<br>In the following figure, we compared the expression of lldPRD operon promoter-GFP and T7-lldPRD operon
 +
promoter-GFP. The T7 promoter could enhance gene expression when the concentration of lactic acid was less than 1 m
 +
mol/L. Considering that the lactate concentration in yogurt is generally no more than 1m mol/L, the conclusion can
 +
given that the T7 promoter can enhance the signal intensity for our experiments
 +
<img src="https://static.igem.org/mediawiki/2018/3/33/T--NEU_China_B--improve3.png">
 +
<img src="https://static.igem.org/mediawiki/2018/c/c6/T--NEU_China_B--improve4.png">
 +
<img src="https://static.igem.org/mediawiki/2018/8/8d/T--NEU_China_B--improve5.png">
 +
</p>
 
</html>
 
</html>

Revision as of 02:11, 16 October 2018

Ruby - Responsive Corporate Tempalte

BBa_k2824008:Lldr-T7-lldPRD operon promoter-GFP


The role of this part is to try to control the concentration of lactic acid on the LLDPRD promoter under the control of quorum sensingg. In the case of LLdr gene expression, the subsequent gene expression was initiated.
This part also is constructed based on the part lldRO1-plldR-lldRO2 (BBa_k82000) . lldRO1-plldR-lldRO2 part is a lactic acid regulated promoter that is open to gene transcription in the presence of lactic acid. Comparing with the T7-lldPRD operon promoter-GFP part, the improvement of this part is that the LLDR is added to part and placed under the control of two promoters respectively to form a double expression regulatory system. This comparison with the two plasmids working together is more convenient for testing the success of the system.
We can easily find that the Lldr-T7-lldPRD operon promoter-GFP part are in a higher level of expression comparing to the lldPRD operon promoter-GFP when the lactate concentration is lower than nearly 1.5m mol/L. Considering that the lactate concentration in yogurt is generally no more than 1m mol/L, the conclusion can given that the T7 promoter can enhance the signal intensity for our experiments.

BBa_k2824006:T7-lldPRD operon promoter-GFP


LLDPRD can sense the presence of lactic acid, in the absence of lactic acid, only a lower background level of expression, but in the presence of lactic acid can open expression, gene transcription. We added the GFP gene into the gene and used green fluorescence as a marker to recognize the presence of lactic acid. In addition, as a strong promoter, T7 can improve the expression level of the target gene.
This part is constructed based on the part lldRO1-plldR-lldRO2 (BBa_k82000) . lldRO1-plldR-lldRO2 part is a lactic acid regulated promoter that is open to gene transcription in the presence of lactic acid. We added a sequence of T 7 promoter and GFP fluorescent protein gene before and after this part Separately. It can effectively improve the expression of the target gene and detect whether the target gene is expressed or not. At the same time, it can accurately determine the amount of the target gene. This allows the overall work of our system to be reflected in digital form.
In the following figure, we compared the expression of lldPRD operon promoter-GFP and T7-lldPRD operon promoter-GFP. The T7 promoter could enhance gene expression when the concentration of lactic acid was less than 1 m mol/L. Considering that the lactate concentration in yogurt is generally no more than 1m mol/L, the conclusion can given that the T7 promoter can enhance the signal intensity for our experiments