Difference between revisions of "Team:Lethbridge/Results"
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| − | <p> | + | <p>Transmission electron microscopy (TEM) confirmed the presence of spherical nanoparticles in both the full-length <i>M. musculus</i> Arc protein and minimal Arc Gag protein samples. Even in the absence of RNA cargo, the Arc-derived PNCs were capable of self-assembly <i>in vitro</i>. In both samples, the average PNC diameter was approximately 30nm +/- 10nm.</p> |
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Revision as of 02:15, 16 October 2018
Purification of Arc Min and Arc Full
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Transmission Electron Microscopy
Transmission electron microscopy (TEM) confirmed the presence of spherical nanoparticles in both the full-length M. musculus Arc protein and minimal Arc Gag protein samples. Even in the absence of RNA cargo, the Arc-derived PNCs were capable of self-assembly in vitro. In both samples, the average PNC diameter was approximately 30nm +/- 10nm.
Full-Length Arc
TEM image (60,000X magnification) of negatively-stained self-assembled full-length Arc PNCs. Scale bar = 200um.
Minimal Arc Gag
TEM image (100,000X magnification) of negatively-stained self-assembled minimal Arc Gag PNCs. Scale bar = 100um.
Mass Spectrometry
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Arc Min
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P22 Capsid
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Analytical Ultracentrifugation of Arc Min
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Transfection of Arc Min
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Purification of P22 and spCas9
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Collaboration
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Improved Parts
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