Difference between revisions of "Team:Toulouse-INSA-UPS/Basic Part"
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<h2 class="heavy">Parts deposed on the registry</h2> | <h2 class="heavy">Parts deposed on the registry</h2> | ||
<h3>Cerberus : A Molecular Binding Plateform (mSA2-CBM3a-AzF)</h3><hr/> | <h3>Cerberus : A Molecular Binding Plateform (mSA2-CBM3a-AzF)</h3><hr/> | ||
| − | <figure class="figure" | + | <div style="text-align:center"> |
| + | <figure class="figure" style="text-align:center;"> | ||
<img style="width : 70%; heigth = auto;" src="https://static.igem.org/mediawiki/2018/4/4f/T--Toulouse-INSA-UPS--Parts--Youn--Cerberus.PNG" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure."> | <img style="width : 70%; heigth = auto;" src="https://static.igem.org/mediawiki/2018/4/4f/T--Toulouse-INSA-UPS--Parts--Youn--Cerberus.PNG" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure."> | ||
| − | <figcaption class="figure-caption"><strong>Figure | + | <figcaption class="figure-caption"><strong>Figure 1:</strong> <i>Part BBa_K2668010</i></figcaption> |
</figure> | </figure> | ||
| + | </div> | ||
<p> | <p> | ||
This part consists on a CBM3a fused with mSA2, an engineered version of Streptavidin and Rhizavidin, on his N terminus endogenous linker and containing a UAG codon on his C terminus linker (stop codon amber) for the incorporation of unnatural amino acid. Note that this part encodes the Cerberus platform if AzF is provided and the appropriate tRNA synthetase is expressed. Otherwise, it encodes for the Orthos construction. | This part consists on a CBM3a fused with mSA2, an engineered version of Streptavidin and Rhizavidin, on his N terminus endogenous linker and containing a UAG codon on his C terminus linker (stop codon amber) for the incorporation of unnatural amino acid. Note that this part encodes the Cerberus platform if AzF is provided and the appropriate tRNA synthetase is expressed. Otherwise, it encodes for the Orthos construction. | ||
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<h3>Sirius: Cellulose-Binding Modules of Type 3a (CBM3a) fused to mRFP1</h3> | <h3>Sirius: Cellulose-Binding Modules of Type 3a (CBM3a) fused to mRFP1</h3> | ||
<hr/> | <hr/> | ||
| − | <figure class="figure" | + | <div style="text-align:center"> |
| + | <figure class="figure" style="text-align:center;"> | ||
<img style="width : 70%; heigth = auto;" src="https://static.igem.org/mediawiki/2018/0/04/T--Toulouse-INSA-UPS--Parts--Youn--Sirius.PNG" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure."> | <img style="width : 70%; heigth = auto;" src="https://static.igem.org/mediawiki/2018/0/04/T--Toulouse-INSA-UPS--Parts--Youn--Sirius.PNG" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure."> | ||
| − | <figcaption class="figure-caption"><strong>Figure | + | <figcaption class="figure-caption"><strong>Figure 2:</strong> <i>Part BBa_K2668020</i></figcaption> |
</figure> | </figure> | ||
| + | </div> | ||
<p> | <p> | ||
This part results of the fusion of the fluorescent protein mRFP1 to the cellulose binding domain CBM3a. The aim of this construct is to prove the binding of CBM3a to cellulose and to quantify it by fluorescence. Furthermore, the mRFP1 is visible to the naked eye and can therefore be used as a visual proof of concept | This part results of the fusion of the fluorescent protein mRFP1 to the cellulose binding domain CBM3a. The aim of this construct is to prove the binding of CBM3a to cellulose and to quantify it by fluorescence. Furthermore, the mRFP1 is visible to the naked eye and can therefore be used as a visual proof of concept | ||
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<h3>mTagBFP AviTag</h3><hr/> | <h3>mTagBFP AviTag</h3><hr/> | ||
| − | <figure class="figure" | + | <div style="text-align:center"> |
| + | <figure class="figure" style="text-align:center;"> | ||
<img style="width : 70%; heigth = auto;" src="https://static.igem.org/mediawiki/2018/d/dc/T--Toulouse-INSA-UPS--Parts--Youn--BFP.PNG" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure."> | <img style="width : 70%; heigth = auto;" src="https://static.igem.org/mediawiki/2018/d/dc/T--Toulouse-INSA-UPS--Parts--Youn--BFP.PNG" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure."> | ||
| − | <figcaption class="figure-caption"><strong>Figure 3 :</strong> Part BBa_K2668070</figcaption> | + | <figcaption class="figure-caption"><strong>Figure 3:</strong> <i>Part BBa_K2668070</i></figcaption> |
</figure> | </figure> | ||
| + | </div> | ||
<p> | <p> | ||
mTag Blue Fluorescent Protein fused with an AviTag for in vivo biotinylation by BirA. | mTag Blue Fluorescent Protein fused with an AviTag for in vivo biotinylation by BirA. | ||
Revision as of 08:45, 16 October 2018
Basic Parts
Parts deposed on the registry
Cerberus : A Molecular Binding Plateform (mSA2-CBM3a-AzF)
This part consists on a CBM3a fused with mSA2, an engineered version of Streptavidin and Rhizavidin, on his N terminus endogenous linker and containing a UAG codon on his C terminus linker (stop codon amber) for the incorporation of unnatural amino acid. Note that this part encodes the Cerberus platform if AzF is provided and the appropriate tRNA synthetase is expressed. Otherwise, it encodes for the Orthos construction.
For more details about the design, refer to the corresponding registry page.
Sirius: Cellulose-Binding Modules of Type 3a (CBM3a) fused to mRFP1
This part results of the fusion of the fluorescent protein mRFP1 to the cellulose binding domain CBM3a. The aim of this construct is to prove the binding of CBM3a to cellulose and to quantify it by fluorescence. Furthermore, the mRFP1 is visible to the naked eye and can therefore be used as a visual proof of concept
For more details about the design, refer to the corresponding registry page.
mTagBFP AviTag
mTag Blue Fluorescent Protein fused with an AviTag for in vivo biotinylation by BirA.
For more details about the design, refer to the corresponding registry page.
No dogs were harmed over the course of this iGEM project.
The whole Toulouse INSA-UPS team wants to thank our sponsors, especially:
And many more. For futher information about our sponsors, please consult our Sponsors page.
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