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<p class="pcontent"> | <p class="pcontent"> | ||
− | Fig | + | Fig 1. Confirmation of prk digestion.</br> |
− | Fig | + | Fig 2. Confirmation of PRK expression in DH5 alpha. The expected protein size is |
37.7kDa. | 37.7kDa. | ||
</p> | </p> | ||
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<p class="pcontent"> | <p class="pcontent"> | ||
− | Fig | + | Fig 3. The result of PRK test in BL21(DE3). The PRK expressing strain is |
incubated | incubated | ||
in both normal M9 medium and altered M9 xylose medium to compare with the | in both normal M9 medium and altered M9 xylose medium to compare with the | ||
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<p class="pcontent"> | <p class="pcontent"> | ||
− | Fig | + | Fig 4. Compares the growth in M9 xylose medium of PR K expressing strain in |
high | high | ||
and low copy number plasmid. The low copy number plasmid, pSB3K3, shows a | and low copy number plasmid. The low copy number plasmid, pSB3K3, shows a | ||
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<p class="pcontent"> | <p class="pcontent"> | ||
− | Fig | + | Fig 5. The result of PRK test in W3110 |
</p></br> | </p></br> | ||
</div> | </div> | ||
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<p class="pcontent"> | <p class="pcontent"> | ||
− | Fig | + | Fig 6. Confirmation of ca digestion</br> |
− | Fig | + | Fig 7. Confirmation of CA expression in BL21(DE3). The expected protein size is |
27.9kDa. | 27.9kDa. | ||
</p></br> | </p></br> | ||
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<p class="pcontent"> | <p class="pcontent"> | ||
− | Fig | + | Fig 8. Confirmation of rbcX and rbcS digestion |
− | Fig | + | Fig 9. Confirmation of RbcX and RbcS expression in BL21(DE3) The expected protein |
size is 15.3 kDA and 13.8kDA respectively. | size is 15.3 kDA and 13.8kDA respectively. | ||
</p> | </p> | ||
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<p class="pcontent"> | <p class="pcontent"> | ||
− | Fig | + | Fig 10. Confirmation of rbcL digestion.</br> |
− | Fig | + | Fig 11. Confirmation of rbcL expression in DH5 alpha. The expected protein size is |
52.37kDa.</br> | 52.37kDa.</br> | ||
</p> | </p> | ||
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<p class="pcontent"> | <p class="pcontent"> | ||
− | Fig | + | Fig 12. shows the dry cell weight of BL21(DE3) incubated in altered M9 xylose |
medium. | medium. | ||
A linear relationship between O.D. and dry cell weight is observed. | A linear relationship between O.D. and dry cell weight is observed. | ||
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<p class="pcontent"> | <p class="pcontent"> | ||
− | Fig | + | Fig 13. Shows the calibration line of DNS assay kit. |
</p></br> | </p></br> | ||
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<p class="pcontent"> | <p class="pcontent"> | ||
− | Fig | + | Fig 14. shows the growth of engineered (contains Rubisco and PRK) W3110(L5T7), BL21(DE3), W3110 incubated in normal |
incubator for 24 hours. The growth of W3110(L5T7) is not obvious while other | incubator for 24 hours. The growth of W3110(L5T7) is not obvious while other | ||
strains show growth after 24hours. | strains show growth after 24hours. | ||
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<p class="pcontent"> | <p class="pcontent"> | ||
− | Fig | + | Fig 15. Shows the growth and XUI measured in 5% CO<sub>2</sub> incubation of 12 |
hours | hours | ||
respectively. Lower growth of the strain that contains. The XUI of the strain | respectively. Lower growth of the strain that contains. The XUI of the strain | ||
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<p class="pcontent"> | <p class="pcontent"> | ||
− | Fig | + | Fig 16. Shows the growth and XUI comparison of each strain. All the tested |
strains are incubated in 5% CO<sub>2</sub> incubator for 12 hr. 0.1mM of IPTG | strains are incubated in 5% CO<sub>2</sub> incubator for 12 hr. 0.1mM of IPTG | ||
was added to | was added to | ||
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<p class="pcontent"> | <p class="pcontent"> | ||
− | Fig | + | Fig 17. Shows the growth and the XUI of BL21(DE3) and W3110 strains. |
</p></br> | </p></br> | ||
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<p class="pcontent"> | <p class="pcontent"> | ||
− | Fig | + | Fig 18. The comparison of the growth and the XUI of the BL21(DE3) that contains |
all three enzymes in normal incubator and 5% CO<sub>2</sub> incubator. The | all three enzymes in normal incubator and 5% CO<sub>2</sub> incubator. The | ||
strain was grown in | strain was grown in | ||
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<p class="pcontent"> | <p class="pcontent"> | ||
− | Fig. | + | Fig 19. The data shows the fluorescent intensity (absorbance: 485 nm, |
excitation: | excitation: | ||
535 nm) expressed by P<sub>asr</sub> in different pH. | 535 nm) expressed by P<sub>asr</sub> in different pH. | ||
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<p class="pcontent"> | <p class="pcontent"> | ||
− | Fig. | + | Fig. 20 The data shows the fluorescent intensity (absorbance: 485 nm, |
excitation: 535 nm) expressed by P<sub>gadA</sub> in different pH. | excitation: 535 nm) expressed by P<sub>gadA</sub> in different pH. | ||
</p> | </p> |
Revision as of 16:05, 16 October 2018
Results