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<h3>Product Design</h3> | <h3>Product Design</h3> | ||
<img class="bigimg" src="https://static.igem.org/mediawiki/2018/2/26/T--NCKU_Tainan--applied_design_product.gif" alt="product design"> | <img class="bigimg" src="https://static.igem.org/mediawiki/2018/2/26/T--NCKU_Tainan--applied_design_product.gif" alt="product design"> | ||
− | <p class="pcenter"> Fig | + | <p class="pcenter">Fig 1. Flow chart of E. coli carbon utilization system </p> |
<ol> | <ol> | ||
<li class="licontent">Overview</li> | <li class="licontent">Overview</li> | ||
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<img class="smallimg" src="https://static.igem.org/mediawiki/2018/6/68/T--NCKU_Tainan--applied_design_overview.png" alt="overview"> | <img class="smallimg" src="https://static.igem.org/mediawiki/2018/6/68/T--NCKU_Tainan--applied_design_overview.png" alt="overview"> | ||
</div> | </div> | ||
− | <p class="pcenter">Fig | + | <p class="pcenter">Fig 2. Overview of the control system </p> |
<p class="pcontent">There are many aspects we need to consider. First, we calculate the emission velocity of CO<sub>2</sub> from the factory, as well as the medium exchange rate and the growth rate of our <i>E. coli</i>. </p> | <p class="pcontent">There are many aspects we need to consider. First, we calculate the emission velocity of CO<sub>2</sub> from the factory, as well as the medium exchange rate and the growth rate of our <i>E. coli</i>. </p> | ||
<p class="pcontent"> | <p class="pcontent"> | ||
− | Fig | + | Fig 1. is a process of whole <i>E. coli</i> carbon utilization that we design for industrial application. We simplify it into three parts which shows in Fig 2. to explain more clearly. Three switches control three parts, named A, B and C. Basically, the factory replaces the medium twice a day. At one hour before replacing the medium, the user needs to turn on switch C to discharge ninety percent of the medium. When it is time to replace the medium, switch C will be turned off and switch B will be turned on to refill medium. When sufficient medium is added, switch B will be turned off and switch A will be turned on to let CO<sub>2</sub> in. Just like the animation showed on Fig 1.. |
</p> | </p> | ||
<p class="pcontent">Considering the cost, the growth time of our <i>E. coli</i> and the floor area, we optimized replace time of the medium, replace it every twelve hours and with 72 parallel bioreactors. | <p class="pcontent">Considering the cost, the growth time of our <i>E. coli</i> and the floor area, we optimized replace time of the medium, replace it every twelve hours and with 72 parallel bioreactors. | ||
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<img class="smallimg" src="https://static.igem.org/mediawiki/2018/4/46/T--NCKU_Tainan--applied_design_gasflow.png" alt="gasflow"> | <img class="smallimg" src="https://static.igem.org/mediawiki/2018/4/46/T--NCKU_Tainan--applied_design_gasflow.png" alt="gasflow"> | ||
</div> | </div> | ||
− | <p class="pcenter"> Fig | + | <p class="pcenter">Fig 3. Diagram of gas preparation system and flow system </p> |
<p class="pcontent">According to IGCC (Integrate Gasification Combined Cycle) flow diagram, the fuel is first converted to syngas which is a mixture of H<sub>2</sub> and CO. The syngas is then burned in a combined cycle consisting of a gas turbine and a steam turbine with a heat recovery steam generator (HRSG). After CO<sub>2</sub> / H<sub>2</sub> separation, IGCC can reach the demand of CO<sub>2</sub> purity including low SOx and NOx emission fraction of allowable limits of bacteria. Finally, the produced flue gas could enter the pipeline leading to the bioreactor. </p> | <p class="pcontent">According to IGCC (Integrate Gasification Combined Cycle) flow diagram, the fuel is first converted to syngas which is a mixture of H<sub>2</sub> and CO. The syngas is then burned in a combined cycle consisting of a gas turbine and a steam turbine with a heat recovery steam generator (HRSG). After CO<sub>2</sub> / H<sub>2</sub> separation, IGCC can reach the demand of CO<sub>2</sub> purity including low SOx and NOx emission fraction of allowable limits of bacteria. Finally, the produced flue gas could enter the pipeline leading to the bioreactor. </p> | ||
<p class="pcontent"> | <p class="pcontent"> | ||
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<img class="smallimg" src="https://static.igem.org/mediawiki/2018/b/b8/T--NCKU_Tainan--IGCC.png" alt="medium"> | <img class="smallimg" src="https://static.igem.org/mediawiki/2018/b/b8/T--NCKU_Tainan--IGCC.png" alt="medium"> | ||
</div> | </div> | ||
− | <p class="pcenter"> Fig | + | <p class="pcenter">Fig 4. IGCC process flow diagram. Source: Vattenfall. (2010) |
Syngas has been treated by sulfur and nitrogen removal, as well as heavy metal removal and cooling tank. Through IGCC process, purified CO<sub>2</sub> in flue gas is allowable for <i>E. coli</i> CO<sub>2</sub> utilizing. </p> | Syngas has been treated by sulfur and nitrogen removal, as well as heavy metal removal and cooling tank. Through IGCC process, purified CO<sub>2</sub> in flue gas is allowable for <i>E. coli</i> CO<sub>2</sub> utilizing. </p> | ||
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<img class="smallimg" src="https://static.igem.org/mediawiki/2018/f/f4/T--NCKU_Tainan--applied_design_medium.png" alt="medium"> | <img class="smallimg" src="https://static.igem.org/mediawiki/2018/f/f4/T--NCKU_Tainan--applied_design_medium.png" alt="medium"> | ||
</div> | </div> | ||
− | <p class="pcenter"> Fig | + | <p class="pcenter">Fig 5. Diagram of medium preparation</p> |
<p class="pcontent">At this stage, we have two sections to consider, medium storage and medium preparation before replacing time.</p> | <p class="pcontent">At this stage, we have two sections to consider, medium storage and medium preparation before replacing time.</p> | ||
<p class="pcontent"> | <p class="pcontent"> | ||
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<img class="smallimg" src="https://static.igem.org/mediawiki/2018/7/7e/T--NCKU_Tainan--applied_design_downstream.png" alt="downstream"> | <img class="smallimg" src="https://static.igem.org/mediawiki/2018/7/7e/T--NCKU_Tainan--applied_design_downstream.png" alt="downstream"> | ||
</div> | </div> | ||
− | <p class="pcenter"> Fig | + | <p class="pcenter">Fig 6. Diagram of downstream process</p> |
<p class="pcontent">We will discharge 90% of the used medium in the bioreactor one hour before new medium flows in. Which means that we let 10% of the culture remain in the bioreactor as seed culture. The effluent medium will be sterilized and filtered in the downstream clean-up tank. At this step, we harvest the bacteria and extracting the by-product such as amino acids, proteins, medicine or bio-fuel. Different extracting process designed depends on different by-product. | <p class="pcontent">We will discharge 90% of the used medium in the bioreactor one hour before new medium flows in. Which means that we let 10% of the culture remain in the bioreactor as seed culture. The effluent medium will be sterilized and filtered in the downstream clean-up tank. At this step, we harvest the bacteria and extracting the by-product such as amino acids, proteins, medicine or bio-fuel. Different extracting process designed depends on different by-product. | ||
</p> | </p> | ||
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<img class="smallimg" src="https://static.igem.org/mediawiki/2018/0/09/T--NCKU_Tainan--Product_MBR.jpg" alt="MBR"> | <img class="smallimg" src="https://static.igem.org/mediawiki/2018/0/09/T--NCKU_Tainan--Product_MBR.jpg" alt="MBR"> | ||
− | <p class="pcenter">Fig | + | <p class="pcenter">Fig 7. Picture of waste water recycle system </p> |
<img class="smallimg" src="https://static.igem.org/mediawiki/2018/1/11/T--NCKU_Tainan--Product_MBRreal.jpg" alt="real MBR"> | <img class="smallimg" src="https://static.igem.org/mediawiki/2018/1/11/T--NCKU_Tainan--Product_MBRreal.jpg" alt="real MBR"> | ||
− | <p class="pcenter">Fig | + | <p class="pcenter">Fig 8. Picture of MBR from KME technology Inc.</p> |
</div> | </div> | ||
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<h3>Application : China Steel</h3> | <h3>Application : China Steel</h3> | ||
<img class="bigimg" src="https://static.igem.org/mediawiki/2018/a/a9/T--NCKU_Tainan--applied_design_chinasteel1.png" alt="china_steel"> | <img class="bigimg" src="https://static.igem.org/mediawiki/2018/a/a9/T--NCKU_Tainan--applied_design_chinasteel1.png" alt="china_steel"> | ||
− | <p class="pcenter">Fig | + | <p class="pcenter">Fig 9. Picture of CSC interview</p> |
<p class="pcontent">Meeting with experts and stakeholders is important in shaping our project to fulfill the needs of our target user. | <p class="pcontent">Meeting with experts and stakeholders is important in shaping our project to fulfill the needs of our target user. | ||
China Steel Corporation is the largest integrated steel Manufacturer in Taiwan. Also, they had been adopting the algal bio-sequestration by | China Steel Corporation is the largest integrated steel Manufacturer in Taiwan. Also, they had been adopting the algal bio-sequestration by | ||
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<img style="width: 70%; height: auto;" src="https://static.igem.org/mediawiki/2018/1/15/T--NCKU_Tainan--applied_design_future_work.png" alt="gasflow"> | <img style="width: 70%; height: auto;" src="https://static.igem.org/mediawiki/2018/1/15/T--NCKU_Tainan--applied_design_future_work.png" alt="gasflow"> | ||
</div> | </div> | ||
− | <p class="pcenter"> Fig. | + | <p class="pcenter">Fig. 10 Diagram of pyruvate in central carbon metabolism </p> |
<p class="pcontent">Furthermore, researchers have successfully constructed pathways produced cellulose and | <p class="pcontent">Furthermore, researchers have successfully constructed pathways produced cellulose and | ||
Poly 3-Hydroxybutyrate-co-3-Hydroxyvalerate through the TCA cycle. | Poly 3-Hydroxybutyrate-co-3-Hydroxyvalerate through the TCA cycle. |
Revision as of 16:12, 16 October 2018