Difference between revisions of "Team:TJU China/Model"

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     <div class="pic"><img src="https://static.igem.org/mediawiki/2018/0/01/T--TJU_China--y1.png"></div>
 
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     <div class="figure">Figure 1: Metabolic pathways related to plasmid#1</div>
 
     <div class="figure">Figure 1: Metabolic pathways related to plasmid#1</div>
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     <div class="word">At the beginning, on the plasmid#1, the promoter $P_{arsR}$ isn't bound with ArsR,thus it is active.ArsR and smURFP are transcribed and translated under the control of the
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        promoters $P_{arsR_{u}}$ and $P_{arsR_{d}}$,with subscript u and d representing upstream and downstream separately.The subscript l of smURFP in the equation means leaky expression without
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        the expression of $As^{3+}$.As ArsR is expressed gradually,it will bind with the promoter $P_{arsR}$ and make it inactive.</div>
  
  
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Revision as of 17:36, 16 October 2018

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Dynamic Model of Heavy Metal Detection Biosensor
Minghui Yin,Sherry Dongqi Bao
TianJin University
October 15,2018
1 Introduction
Modeling is a powerful tool in synthetic biology. It provides us with a necessary engineering approach to characterize our pathways quantitatively and predict their performance,thus help us test and modify our design.Through the dynamic model of heavy-metal detection biosensor,we hope to gain insights into the characteristics of our whole circuit's dynamics.
2 Methods
2.1 Analysis of metabolic pathways
Figure 1: Metabolic pathways related to plasmid#1
At the beginning, on the plasmid#1, the promoter $P_{arsR}$ isn't bound with ArsR,thus it is active.ArsR and smURFP are transcribed and translated under the control of the promoters $P_{arsR_{u}}$ and $P_{arsR_{d}}$,with subscript u and d representing upstream and downstream separately.The subscript l of smURFP in the equation means leaky expression without the expression of $As^{3+}$.As ArsR is expressed gradually,it will bind with the promoter $P_{arsR}$ and make it inactive.