Difference between revisions of "Team:NCTU Formosa/Collaborations"

On August 23^th, we prepared a wiki course for NTHU_Formosa, helping them to troubleshoot on their difficulties of writing their wiki. First of all, we taught them the concept and process of designing a website as an introduction. Second, we introduced how to use HTML, CSS, as well as JavaScript. Third, we helped them to build up a simple RWD website step by step to make them more familiar with the web languages. Finally, we gave them some tips and advices on editing their wiki. We’re really happy about having such a chance to share our knowledge with other iGEM teams! Hope the course could help them in a meaningful way. We’re looking forward to have further collaboration with NTHU_Formosa.

Figure 1: The wiki course (when talking about CSS)

Figure 2: NCTU_Formosa and NTHU_Formosa team members

Characterization of Parts from NTHU_Formosa

We characterize the part of NTHU_Formosa team. These inserted vectors, including pCAG-GBP, pTRE3G-LuxAB, pCMV-LuxCDEfrp, pTRE3G-mCherry, are to produce receptor, enzyme, substrate, which are essential in the process of emitting the light. We helped with NTHU_Formosa to check if these vectors are correct. The results in the figure below shows these vectors are connected correctly.

Figure 3: The wiki course (when talking about CSS)

Figure 4: The wiki course (when talking about CSS)

Figure 5: The wiki course (when talking about CSS)

Bidirectional collaboration with NTHU_Formosa

After inducing our E. coli, we wanted double check that our constructs were correctly expressed. To do this, we invited members of team NTHU_Formosa to come to our lab on October 6^th, where they spent an afternoon running an SDS-PAGE of Enterocin B and Enterocin 96. The results are in the figures below. Thanks to NTHU_Formosa’s help, we confirmed that our peptides were being expressed correctly.

Figure 6: The wiki course (when talking about CSS)

Figure 7: The wiki course (when talking about CSS)

Template

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      <div class="block content_1">
        <p>
-         &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We characterize the part of NTHU_Formosa team. These inserted constructs, including <a href="http://parts.igem.org/Part:BBa_K2635001">pCAG-GBP</a>, pTRE3G-LuxAB, pCMV-LuxCDEfrp, <a href="http://parts.igem.org/Part:BBa_K2635000">pTRE3G-mCherry</a>, are to produce receptor, enzyme, substrate, which are essential in the process of emitting the light. We helped with NTHU_Formosa to check if these constructs are correct. The results in the figure below shows the correct (expected) DNA fragments representing each inserts and vectors.
+         &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We characterize the part of NTHU_Formosa team. These inserted vectors, including <a href="http://parts.igem.org/Part:BBa_K2635001">pCAG-GBP</a>, pTRE3G-LuxAB, pCMV-LuxCDEfrp, <a href="http://parts.igem.org/Part:BBa_K2635000">pTRE3G-mCherry</a>, are to produce receptor, enzyme, substrate, which are essential in the process of emitting the light. We helped with NTHU_Formosa to check if these vectors are correct. The results in the figure below shows these vectors are connected correctly.
        </p>
      </div>
@@ Line 237: / Line 237: @@
      <div class="explanation">
        <svg class="icon" aria-hidden="true" data-prefix="fas" data-icon="arrow-circle-up" class="svg-inline--fa fa-arrow-circle-up fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M8 256C8 119 119 8 256 8s248 111 248 248-111 248-248 248S8 393 8 256zm143.6 28.9l72.4-75.5V392c0 13.3 10.7 24 24 24h16c13.3 0 24-10.7 24-24V209.4l72.4 75.5c9.3 9.7 24.8 9.9 34.3.4l10.9-11c9.4-9.4 9.4-24.6 0-33.9L273 107.7c-9.4-9.4-24.6-9.4-33.9 0L106.3 240.4c-9.4 9.4-9.4 24.6 0 33.9l10.9 11c9.6 9.5 25.1 9.3 34.4-.4z"></path></svg>
-       Figure 3: The Gel electrophoresis result
+       Figure 3: The wiki course (when talking about CSS)
      </div>
      <img src="https://static.igem.org/mediawiki/2018/7/7c/T--NCTU_Formosa--collaboration4.jpg" class="photo_1">
      <div class="explanation">
        <svg class="icon" aria-hidden="true" data-prefix="fas" data-icon="arrow-circle-up" class="svg-inline--fa fa-arrow-circle-up fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M8 256C8 119 119 8 256 8s248 111 248 248-111 248-248 248S8 393 8 256zm143.6 28.9l72.4-75.5V392c0 13.3 10.7 24 24 24h16c13.3 0 24-10.7 24-24V209.4l72.4 75.5c9.3 9.7 24.8 9.9 34.3.4l10.9-11c9.4-9.4 9.4-24.6 0-33.9L273 107.7c-9.4-9.4-24.6-9.4-33.9 0L106.3 240.4c-9.4 9.4-9.4 24.6 0 33.9l10.9 11c9.6 9.5 25.1 9.3 34.4-.4z"></path></svg>
-       Figure 4: The experiment photo (loading sample)
+       Figure 4: The wiki course (when talking about CSS)
      </div>
      <img src="https://static.igem.org/mediawiki/2018/c/c1/T--NCTU_Formosa--collaboration3.jpg" class="photo_1">
      <div class="explanation" style="margin-bottom: 7vw;">
        <svg class="icon" aria-hidden="true" data-prefix="fas" data-icon="arrow-circle-up" class="svg-inline--fa fa-arrow-circle-up fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M8 256C8 119 119 8 256 8s248 111 248 248-111 248-248 248S8 393 8 256zm143.6 28.9l72.4-75.5V392c0 13.3 10.7 24 24 24h16c13.3 0 24-10.7 24-24V209.4l72.4 75.5c9.3 9.7 24.8 9.9 34.3.4l10.9-11c9.4-9.4 9.4-24.6 0-33.9L273 107.7c-9.4-9.4-24.6-9.4-33.9 0L106.3 240.4c-9.4 9.4-9.4 24.6 0 33.9l10.9 11c9.6 9.5 25.1 9.3 34.4-.4z"></path></svg>
-       Figure 5: The experiment photo (watching the result)
+       Figure 5: The wiki course (when talking about CSS)
      </div>
@@ Line 259: / Line 259: @@
      <div class="explanation">
        <svg class="icon" aria-hidden="true" data-prefix="fas" data-icon="arrow-circle-up" class="svg-inline--fa fa-arrow-circle-up fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M8 256C8 119 119 8 256 8s248 111 248 248-111 248-248 248S8 393 8 256zm143.6 28.9l72.4-75.5V392c0 13.3 10.7 24 24 24h16c13.3 0 24-10.7 24-24V209.4l72.4 75.5c9.3 9.7 24.8 9.9 34.3.4l10.9-11c9.4-9.4 9.4-24.6 0-33.9L273 107.7c-9.4-9.4-24.6-9.4-33.9 0L106.3 240.4c-9.4 9.4-9.4 24.6 0 33.9l10.9 11c9.6 9.5 25.1 9.3 34.4-.4z"></path></svg>
-       Figure 6: The experiment photo (making SDS-PAGE gel)
+       Figure 6: The wiki course (when talking about CSS)
      </div>
      <img src="https://static.igem.org/mediawiki/2018/6/66/T--NCTU_Formosa--collaboration1.jpg" class="photo_1">
      <div class="explanation" style="margin-bottom: 7vw;">
        <svg class="icon" aria-hidden="true" data-prefix="fas" data-icon="arrow-circle-up" class="svg-inline--fa fa-arrow-circle-up fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M8 256C8 119 119 8 256 8s248 111 248 248-111 248-248 248S8 393 8 256zm143.6 28.9l72.4-75.5V392c0 13.3 10.7 24 24 24h16c13.3 0 24-10.7 24-24V209.4l72.4 75.5c9.3 9.7 24.8 9.9 34.3.4l10.9-11c9.4-9.4 9.4-24.6 0-33.9L273 107.7c-9.4-9.4-24.6-9.4-33.9 0L106.3 240.4c-9.4 9.4-9.4 24.6 0 33.9l10.9 11c9.6 9.5 25.1 9.3 34.4-.4z"></path></svg>
-       Figure 7: The experiment photo (loading sample)
+       Figure 7: The wiki course (when talking about CSS)
      </div>