Difference between revisions of "Team:Newcastle/Protocols"
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<td>Chemotaxis</td> | <td>Chemotaxis</td> | ||
<td><a href="https://static.igem.org/mediawiki/2018/e/eb/T--Newcastle--MinimalAgar.docx" style="color:green">View</a></td> | <td><a href="https://static.igem.org/mediawiki/2018/e/eb/T--Newcastle--MinimalAgar.docx" style="color:green">View</a></td> | ||
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| + | <td>0.75% Agar Chemotaxis Assay</td> | ||
| + | <td>Connor Trotter</td> | ||
| + | <td>Chemotaxis</td> | ||
| + | <td><a href="https://static.igem.org/mediawiki/2018/b/bf/T--Newcastle--AgarV1.docx" style="color:green">View<a/></td> | ||
</tr> | </tr> | ||
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Revision as of 21:22, 16 October 2018
Experiments
Protocols
EXPERIMENTS
PROTOCOLS
| Protocol | Author | Category | PDF File |
|---|---|---|---|
| Glycerol Stock Preserves | Connor Trotter | General | View |
| Bacterial Isolation from Freeze-Dried Pellet | Connor Trotter | General | View |
| Cell Counting | Connor Trotter | Chemotaxis | View |
| Minimal A Salt Agar Chemotaxis Assay | Connor Trotter | Chemotaxis | View |
| 0.75% Agar Chemotaxis Assay | Connor Trotter | Chemotaxis | View |
| Growth Curve in Liquid Culture | Sadiya Quazi | Chemotaxis | View |
| Naringenin Kill Curve in Liquid Culture | Sadiya Quazi | Chemotaxis | View |
| Planting Seeds in Agar-filled Microcentrifuge tubes | Lewis Tomlinson | Endophyte Development | View |
| Preparation and Staining of Microscopy samples | Lewis Tomlinson | Endophyte Development | View |
| Planting Seeds in Agar-filled pipette-tip racks | Lewis Tomlinson | Endophyte Development | View |
| Re-isolating endophytes | Lewis Tomlinson | Endophyte Development | View |
| Electroporation of Pseudomonas sp. | Frank Eardley | Endophyte Development | View |
| MIC Opentrons Code (2 Plates) | Frank Eardley | Endophyte Development | View |
| Amplification of gblocks and gradient PCR | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Colony PCR of transformed DH5α cells | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| DPN1 digestion and purification | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Gel extraction and purification of the gblocks | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Gibson Assembly of gblocks and pSB1C3 backbone | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Making chemically competent cells | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Miniprep of transformed colonies | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Amplification, Purification and Quantification of the pSB1C3 backbone by PCR | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Quantifying DNA concentration | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Resuspending gblocks and primers | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| SOC media preparation | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Transformation of the gblocks into pSB1C3 | Heather Bottomley | Naringenin Operon Biosynthesis | View |