Difference between revisions of "Team:Newcastle/Protocols"
Alice Banks (Talk | contribs) m |
|||
| Line 115: | Line 115: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Planting Seeds in Agar- | + | <td>Planting Seeds in Agar-Filled Microcentrifuge Tubes</td> |
<td>Lewis Tomlinson</td> | <td>Lewis Tomlinson</td> | ||
<td>Endophyte Development</td> | <td>Endophyte Development</td> | ||
| Line 121: | Line 121: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Preparation and Staining of Microscopy | + | <td>Preparation and Staining of Microscopy Samples</td> |
<td>Lewis Tomlinson</td> | <td>Lewis Tomlinson</td> | ||
<td>Endophyte Development</td> | <td>Endophyte Development</td> | ||
| Line 127: | Line 127: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Planting Seeds in Agar- | + | <td>Planting Seeds in Agar-Filled Pipette-Tip Racks</td> |
<td>Lewis Tomlinson</td> | <td>Lewis Tomlinson</td> | ||
<td>Endophyte Development</td> | <td>Endophyte Development</td> | ||
| Line 133: | Line 133: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Re-isolating | + | <td>Re-isolating Endophytes</td> |
<td>Lewis Tomlinson</td> | <td>Lewis Tomlinson</td> | ||
<td>Endophyte Development</td> | <td>Endophyte Development</td> | ||
| Line 151: | Line 151: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Amplification of | + | <td>Amplification of gBlocks and Gradient PCR</td> |
<td>Heather Bottomley</td> | <td>Heather Bottomley</td> | ||
<td>Naringenin Operon Biosynthesis</td> | <td>Naringenin Operon Biosynthesis</td> | ||
| Line 157: | Line 157: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Colony PCR of | + | <td>Colony PCR of Transformed DH5α Cells</td> |
<td>Heather Bottomley</td> | <td>Heather Bottomley</td> | ||
<td>Naringenin Operon Biosynthesis</td> | <td>Naringenin Operon Biosynthesis</td> | ||
| Line 163: | Line 163: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>DpnI Digestion and Purification</td> |
<td>Heather Bottomley</td> | <td>Heather Bottomley</td> | ||
<td>Naringenin Operon Biosynthesis</td> | <td>Naringenin Operon Biosynthesis</td> | ||
| Line 169: | Line 169: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Gel | + | <td>Gel Extraction and Purification of the gBlocks</td> |
<td>Heather Bottomley</td> | <td>Heather Bottomley</td> | ||
<td>Naringenin Operon Biosynthesis</td> | <td>Naringenin Operon Biosynthesis</td> | ||
| Line 175: | Line 175: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Gibson Assembly of | + | <td>Gibson Assembly of gBlocks and pSB1C3 Backbone</td> |
<td>Heather Bottomley</td> | <td>Heather Bottomley</td> | ||
<td>Naringenin Operon Biosynthesis</td> | <td>Naringenin Operon Biosynthesis</td> | ||
| Line 181: | Line 181: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Making | + | <td>Making Chemically Competent Cells</td> |
<td>Heather Bottomley</td> | <td>Heather Bottomley</td> | ||
<td>Naringenin Operon Biosynthesis</td> | <td>Naringenin Operon Biosynthesis</td> | ||
| Line 187: | Line 187: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Miniprep of | + | <td>Miniprep of Transformed Colonies</td> |
<td>Heather Bottomley</td> | <td>Heather Bottomley</td> | ||
<td>Naringenin Operon Biosynthesis</td> | <td>Naringenin Operon Biosynthesis</td> | ||
| Line 193: | Line 193: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Amplification, Purification and Quantification of the pSB1C3 | + | <td>Amplification, Purification and Quantification of the pSB1C3 Backbone by PCR</td> |
<td>Heather Bottomley</td> | <td>Heather Bottomley</td> | ||
<td>Naringenin Operon Biosynthesis</td> | <td>Naringenin Operon Biosynthesis</td> | ||
| Line 199: | Line 199: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Quantifying DNA | + | <td>Quantifying DNA Concentration</td> |
<td>Heather Bottomley</td> | <td>Heather Bottomley</td> | ||
<td>Naringenin Operon Biosynthesis</td> | <td>Naringenin Operon Biosynthesis</td> | ||
| Line 205: | Line 205: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Resuspending | + | <td>Resuspending gBlocks and Primers</td> |
<td>Heather Bottomley</td> | <td>Heather Bottomley</td> | ||
<td>Naringenin Operon Biosynthesis</td> | <td>Naringenin Operon Biosynthesis</td> | ||
| Line 211: | Line 211: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>SOC | + | <td>SOC Media Preparation</td> |
<td>Heather Bottomley</td> | <td>Heather Bottomley</td> | ||
<td>Naringenin Operon Biosynthesis</td> | <td>Naringenin Operon Biosynthesis</td> | ||
| Line 217: | Line 217: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Transformation of the | + | <td>Transformation of the gBlocks into pSB1C3</td> |
<td>Heather Bottomley</td> | <td>Heather Bottomley</td> | ||
<td>Naringenin Operon Biosynthesis</td> | <td>Naringenin Operon Biosynthesis</td> | ||
Revision as of 00:19, 17 October 2018
Experiments
Protocols
EXPERIMENTS
PROTOCOLS
| Protocol | Author | Category | PDF File |
|---|---|---|---|
| Glycerol Stock Preserves | Connor Trotter | General | View |
| Bacterial Isolation from Freeze-Dried Pellet | Connor Trotter | General | View |
| Cell Counting | Connor Trotter | Chemotaxis | View |
| Minimal A Salt Agar Chemotaxis Assay | Connor Trotter | Chemotaxis | View |
| 0.75% Agar Chemotaxis Assay | Connor Trotter | Chemotaxis | View |
| Growth Curve in Liquid Culture | Sadiya Quazi | Chemotaxis | View |
| Naringenin Kill Curve in Liquid Culture | Sadiya Quazi | Chemotaxis | View |
| Planting Seeds in Agar-Filled Microcentrifuge Tubes | Lewis Tomlinson | Endophyte Development | View |
| Preparation and Staining of Microscopy Samples | Lewis Tomlinson | Endophyte Development | View |
| Planting Seeds in Agar-Filled Pipette-Tip Racks | Lewis Tomlinson | Endophyte Development | View |
| Re-isolating Endophytes | Lewis Tomlinson | Endophyte Development | View |
| Electroporation of Pseudomonas sp. | Frank Eardley | Endophyte Development | View |
| MIC Opentrons Code (2 Plates) | Frank Eardley | Endophyte Development | View |
| Amplification of gBlocks and Gradient PCR | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Colony PCR of Transformed DH5α Cells | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| DpnI Digestion and Purification | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Gel Extraction and Purification of the gBlocks | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Gibson Assembly of gBlocks and pSB1C3 Backbone | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Making Chemically Competent Cells | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Miniprep of Transformed Colonies | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Amplification, Purification and Quantification of the pSB1C3 Backbone by PCR | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Quantifying DNA Concentration | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Resuspending gBlocks and Primers | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| SOC Media Preparation | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Transformation of the gBlocks into pSB1C3 | Heather Bottomley | Naringenin Operon Biosynthesis | View |