Attributions
We are so grateful for receiving tremendous help from these amazing people on the way turning our idea into reality. You have been not only our helper but also our inspiration and emotional support that motivate us on completing a nearly impossible mission. We couldn’t have accomplished anything without you. We owe you our best thanks.
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Team Work
Ideas
Our project came out from months of brainstorming and was first proposed by Kairan Zhang on June with solid foundation laid after great amount of synthetic biology-related readings. Being the core of this project Zhang has been continuously giving new thoughts as well as advice on the project design throughout the season, and has carried out great improvement on our device.
Biobricks
Peixiang He, Zhihan Liang, Zhixiang Liu and Yirui Hong were mainly responsible for biobrick-related works in wet lab. They were capable of engineering unique bacteria that express exactly how we wanted them to. It is also their efforts on preparing engineered bacteria for further properties examination in cell and mouse experiments.
Cell Experiments
Cancer cell lines HT29 and normal epithelial cell lines HACAT were cultured by Yuncong Ran, Shiyu Sun, Ruoyu Xia and Shihao Bai to serve as the subjects for multiple short peptides adhesive property examinations. The examinations aimed to testify the specificity of antigen-antibody binding between our bacteria and different cell lines.
Ultrasound imaging
Ultrasound-related experiments were mainly designed and performed by Qingwei. He also developed the Matlab code for ultrasound image porcessing job. Shiyu Sun helped to do this job when Qingwei was busy with the team wiki.
MRI imaging
Qingwei Fang recorded MRI imaging experiment and assisted in experiment design and data processing. Kuan Yao helped record the experiments when Qingwei was busy with wiki development.
Modeling
Shuyu Zheng, Shiyu Sun, Xin Zhang, Anyuan Tian and Yulin Liao did all the modeling work for our projects.
Human Practices
Bozitao Zhong, Weiwen Wu, Kuan Yao and Shihao Bai were in charge of the human practices part. Bozitao Zhong was the leader of this part. Weiwen Wu brought up a lot of new ideas. Kuan Yao organized the exhibition and Shihao Bai helped the research in Canada.
Art
Our team’s logo, banner, posters, uniforms and the layout of our wiki were all designed by Weiwen Wu. She helped us to rank the second best poster in the fifth CCiC. Yuncong Ran also helped to design the posters exhibited in our 10th anniversary exhibition.
Wiki
Our wiki were developed by all of us but Qingwei Fang and Yulin Liao construct the website’s frame for us in advance. Qingwei were in charge with communicating with the designer Weiwen and the development of wiki on computers while Yulin worked on the mobile web.
Presentation
Jiayi Xiao is our presentation mastermind. She attended the Asia-Pacific iGEM Conference on behalf of our team. Later, she instructed the presentation on the fifth CCiC. Our last presentation on Giant Jamboree was also conducted by her. Her speech is really appealing and full of passion.
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Advisor Support
Zhijun Wang
Zhijun Wang helped the preparation of iGEM 2018 and clarified some confusion related to ultrasound and modeling. He also gave advice on the construction of our Wiki.
Zhuoyang Chen
He helped with troubleshoot in the wet lab. Furthermore, he helped the team work, keeping everything in progress and provided information about the competition.
Chang Gao
Chang Gao was an active team member last year, so her experiences were used to determine project, design and arrange experiments and so on. She gave many protocols of experiments as well, such as constructing plasmids, western blotting. Besides, she helped finish the western blotting.
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Supervisor Support
Lin He
Lin He is the supervisor of our team and the director of Bio-X, who gave us funding and university-related support. He founded iGEM team SJTU-BioX-Shagnhai ten years ago and has been supporting us since then.
Gang Ma
Gang Ma is the supervisor of our team and the professor of Bio-X, who gave us advice and instructions. He cheerer us up when we felt frustrated. He encouraged us to overcome our limitations and keep trying for the best.
Yushu Wang
Yushu Wang spent a lot of time and energy on us, helping with all kinds of problems, from experiments materials supply to troubleshooting. She is just like a sister to all of us, so warm and kind.
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General Support
Research institute of Bio-X
During the whole preparation procedure of the iGEM competition, Bio-X offered us a great support in all aspects. Thanks to their providing of experimental funds and drugs.
Shanghai Jiao Tong University
Our university gives us logistics support for the whole project. Thanks for the substantial support given by our university.
School of Life Sciences and Biotechnology, SJTU
During the whole experimentation, School of Life Science and Biotechnology provided us with experimental funds, laboratory and experimental apparatus.
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Technique Support
MRI imaging--Prof. Yiping Du
The MRI experiment was directed by Professor Yiping Du, Dean of the IMIT (Institute of Medical Imaging Technology), Shanghai Jiaotong University. Zhang Yufei designed, conducted experiments and completed data processing.
CRC induction--Qing Yu
Yu is an doctoral student who focus on studies related to colorectal cancer mouse model. He is our advicer for the CRC induction process as well as other related experiments. To be precise he offers help on CRC induction, sectioning, as well as cytometry.
Clinical ultrasonic equipment--Doctor Tian Tsai
Doctor Tsai is a veterinarian in HongBiZi pet clinic. He has offered us clinical ultrasound equipment free of charge and has given us very useful clinical advice on examining real animals in vitro.
Overall support--Qing Chun Yang
Yang is a doctoral student in our PI’s group who has long been very kind and helpful on our project. As a senior, she helped us with the orientation in the lab while she also offers tremendous help and advice on all molecular, cell and mouse experiments, including immunohistochemistry, paraffin sectioning, and bacteria transduction. She enlightens us on trying new procedures to better demonstrate our examination and has long been our inspiration and support.
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Materials Support
Xia Zhang
Xia Zhang is a teacher in SJTU Experimental Teaching Center. She provided us with plasmid containing egfp gene which was used in the adhesion experiments.
Gengming Niu
Gengming Niu, from the Fifth People’s Hospital of Shanghai, Fudan University provided the human cell lines HT29 to us. We used it to test our system in vitro.
2018 iGEM teams
We also got materials support from 2018 iGEM teams, further details can be found atCollabrations page.
