Difference between revisions of "Team:Newcastle/Protocols"
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<td>Planting Seeds in Agar-Filled Microcentrifuge Tubes</td> | <td>Planting Seeds in Agar-Filled Microcentrifuge Tubes</td> | ||
<td>Lewis Tomlinson</td> | <td>Lewis Tomlinson</td> | ||
| − | <td> | + | <td>Root Colonisation</td> |
<td><a href="https://static.igem.org/mediawiki/2018/5/5a/T--Newcastle--MCPlanting.docx" style="color:green">View</a></td> | <td><a href="https://static.igem.org/mediawiki/2018/5/5a/T--Newcastle--MCPlanting.docx" style="color:green">View</a></td> | ||
</tr> | </tr> | ||
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<td>Preparation and Staining of Microscopy Samples</td> | <td>Preparation and Staining of Microscopy Samples</td> | ||
<td>Lewis Tomlinson</td> | <td>Lewis Tomlinson</td> | ||
| − | <td> | + | <td>Root Colonisation</td> |
<td><a href="https://static.igem.org/mediawiki/2018/6/69/T--Newcastle--Microscopy.docx" style="color:green">View</a></td> | <td><a href="https://static.igem.org/mediawiki/2018/6/69/T--Newcastle--Microscopy.docx" style="color:green">View</a></td> | ||
</tr> | </tr> | ||
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<td>Planting Seeds in Agar-Filled Pipette-Tip Racks</td> | <td>Planting Seeds in Agar-Filled Pipette-Tip Racks</td> | ||
<td>Lewis Tomlinson</td> | <td>Lewis Tomlinson</td> | ||
| − | <td> | + | <td>Root Colonisation</td> |
<td><a href="https://static.igem.org/mediawiki/2018/9/9e/T--Newcastle--Rack.docx" style="color:green">View</a></td> | <td><a href="https://static.igem.org/mediawiki/2018/9/9e/T--Newcastle--Rack.docx" style="color:green">View</a></td> | ||
</tr> | </tr> | ||
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<td>Re-isolating Endophytes</td> | <td>Re-isolating Endophytes</td> | ||
<td>Lewis Tomlinson</td> | <td>Lewis Tomlinson</td> | ||
| − | <td> | + | <td>Root Colonisation</td> |
<td><a href="https://static.igem.org/mediawiki/2018/d/d7/T--Newcastle--ReIsolate.docx" style="color:green">View</a></td> | <td><a href="https://static.igem.org/mediawiki/2018/d/d7/T--Newcastle--ReIsolate.docx" style="color:green">View</a></td> | ||
</tr> | </tr> | ||
Revision as of 10:59, 17 October 2018
Experiments
Protocols
EXPERIMENTS
PROTOCOLS
| Protocol | Author | Category | PDF File |
|---|---|---|---|
| Glycerol Stock Preserves | Connor Trotter | General | View |
| Bacterial Isolation from Freeze-Dried Pellet | Connor Trotter | General | View |
| Cell Counting | Connor Trotter | Chemotaxis | View |
| Minimal A Salt Agar Chemotaxis Assay | Connor Trotter | Chemotaxis | View |
| 0.75% Agar Chemotaxis Assay | Connor Trotter | Chemotaxis | View |
| Growth Curve in Liquid Culture | Sadiya Quazi | Chemotaxis | View |
| Naringenin Kill Curve in Liquid Culture | Sadiya Quazi | Chemotaxis | View |
| Planting Seeds in Agar-Filled Microcentrifuge Tubes | Lewis Tomlinson | Root Colonisation | View |
| Preparation and Staining of Microscopy Samples | Lewis Tomlinson | Root Colonisation | View |
| Planting Seeds in Agar-Filled Pipette-Tip Racks | Lewis Tomlinson | Root Colonisation | View |
| Re-isolating Endophytes | Lewis Tomlinson | Root Colonisation | View |
| Electroporation of Pseudomonas sp. | Frank Eardley | Endophytic Chassis Development | View |
| MIC Opentrons Code (2 Plates) | Frank Eardley | Endophytic Chassis Development | View |
| MIC Protocol | Frank Eardley | Endophytic Chassis Development | View |
| 2-Part Gibson Assembly | Frank Eardley | Endophytic Chassis Development | View |
| Amplification of gBlocks and Gradient PCR | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Colony PCR of Transformed DH5α Cells | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| DpnI Digestion and Purification | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Gel Extraction and Purification of the gBlocks | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Gibson Assembly of gBlocks and pSB1C3 Backbone | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Making Chemically Competent Cells | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Miniprep of Transformed Colonies | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Amplification, Purification and Quantification of the pSB1C3 Backbone by PCR | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Quantifying DNA Concentration | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Resuspending gBlocks and Primers | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| SOC Media Preparation | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Transformation of the gBlocks into pSB1C3 | Heather Bottomley | Naringenin Operon Biosynthesis | View |