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− | body{ | + | <!DOCTYPE > |
− | + | <html> | |
− | + | ||
− | } | + | <head> |
− | .notebook_month{ | + | <meta http-equiv="Content-Type" content="text/html; charset=UTF-8"> |
− | + | <link rel="stylesheet" href="https://2018.igem.org/Template:TJU_China/default_CSS?action=raw&ctype=text/css" type="text/css"> | |
− | + | <link rel="stylesheet" href="https://2018.igem.org/Template:TJU_China/nav_css?action=raw&ctype=text/css" type="text/css"> | |
− | + | <style> | |
− | + | body { | |
− | + | background: url("./img/notebook_group_bg.png"); | |
− | + | background-size: 100%; | |
− | } | + | } |
− | . | + | |
− | + | .notebook_month { | |
− | } | + | font-style: italic; |
− | .notebook_timeline{ | + | margin-left: 50px; |
− | + | float: left; | |
− | + | width: 50px; | |
− | + | margin-top: 4px; | |
− | + | position: fixed; | |
− | + | } | |
− | + | ||
− | + | .notebook_month_p { | |
− | + | margin-top: 55px; | |
− | + | font-size: 22px; | |
− | } | + | } |
− | + | ||
− | + | .notebook_timeline { | |
− | + | position: fixed; | |
− | + | float: left; | |
− | .notebook_button_list button{ | + | width: 6px; |
− | + | height: 660px; | |
− | + | background: #4e72b8; | |
− | + | margin-left: 170px; | |
− | + | } | |
− | + | ||
− | + | .notebook_button_list { | |
− | + | position: fixed; | |
− | + | margin-left: 100px; | |
− | + | } | |
+ | |||
+ | .notebook_button_list button { | ||
+ | float: left; | ||
+ | height: 30px; | ||
+ | width: 30px; | ||
+ | border-radius: 50%; | ||
+ | border: none; | ||
+ | background: -webkit-linear-gradient(left, #81C0C0, #D2A2CC); | ||
+ | /* Safari 5.1 - 6.0 */ | ||
+ | background: -o-linear-gradient(right, #81C0C0, #D2A2CC); | ||
+ | /* Opera 11.1 - 12.0 */ | ||
+ | background: -moz-linear-gradient(right, #81C0C0, #D2A2CC); | ||
+ | /* Firefox 3.6 - 15 */ | ||
+ | background: linear-gradient(to right, #81C0C0, #D2A2CC); | ||
+ | /* 标准的语法(必须放在最后) */ | ||
+ | } | ||
+ | |||
+ | .notebook_button_list button:hover { | ||
+ | background: -webkit-linear-gradient(left, #005757, #8600FF); | ||
+ | /* Safari 5.1 - 6.0 */ | ||
+ | background: -o-linear-gradient(right, #005757, #8600FF); | ||
+ | /* Opera 11.1 - 12.0 */ | ||
+ | background: -moz-linear-gradient(right, #005757, #8600FF); | ||
+ | /* Firefox 3.6 - 15 */ | ||
+ | background: linear-gradient(to right, #005757, #8600FF); | ||
+ | /* 标准的语法(必须放在最后) */ | ||
+ | } | ||
+ | |||
+ | .notebook_button1 { | ||
+ | outline: none; | ||
+ | position: relative; | ||
+ | left: 58px; | ||
+ | top: 54px; | ||
+ | } | ||
+ | |||
+ | .notebook_button2 { | ||
+ | outline: none; | ||
+ | position: relative; | ||
+ | left: 28px; | ||
+ | top: 127px; | ||
+ | } | ||
+ | |||
+ | .notebook_button3 { | ||
+ | outline: none; | ||
+ | position: relative; | ||
+ | left: -2px; | ||
+ | top: 200px; | ||
+ | } | ||
+ | |||
+ | .notebook_button4 { | ||
+ | outline: none; | ||
+ | position: relative; | ||
+ | left: -32px; | ||
+ | top: 275px; | ||
+ | } | ||
+ | |||
+ | .notebook_button5 { | ||
+ | outline: none; | ||
+ | position: relative; | ||
+ | left: -62px; | ||
+ | top: 347px; | ||
+ | } | ||
+ | |||
+ | .notebook_button6 { | ||
+ | outline: none; | ||
+ | position: relative; | ||
+ | left: -92px; | ||
+ | top: 418px; | ||
+ | } | ||
+ | |||
+ | .notebook_button7 { | ||
+ | outline: none; | ||
+ | position: relative; | ||
+ | left: -122px; | ||
+ | top: 493px; | ||
+ | } | ||
+ | |||
+ | .notebook_button8 { | ||
+ | outline: none; | ||
+ | position: relative; | ||
+ | left: -152px; | ||
+ | top: 565px; | ||
+ | } | ||
+ | |||
+ | #notebook_March { | ||
+ | display: block; | ||
+ | } | ||
+ | |||
+ | #notebook_April { | ||
+ | display: none; | ||
+ | } | ||
+ | |||
+ | #notebook_May { | ||
+ | display: none; | ||
+ | } | ||
+ | |||
+ | #notebook_June { | ||
+ | display: none; | ||
+ | } | ||
+ | |||
+ | #notebook_July { | ||
+ | display: none; | ||
+ | } | ||
+ | |||
+ | #notebook_August { | ||
+ | display: none; | ||
+ | } | ||
+ | |||
+ | #notebook_September { | ||
+ | display: none; | ||
+ | } | ||
+ | |||
+ | #notebook_October { | ||
+ | display: none; | ||
+ | } | ||
+ | |||
+ | .notebook_information { | ||
+ | float: left; | ||
+ | margin-left: 300px; | ||
+ | margin-top: 100px; | ||
+ | } | ||
+ | |||
+ | .notebook_week { | ||
+ | margin-top: 25px; | ||
+ | } | ||
+ | |||
+ | .notebook_week_p { | ||
+ | text-align: left; | ||
+ | font-size: 20px; | ||
+ | text-indent: 2em; | ||
+ | margin-top: 30px; | ||
+ | line-height: 1.5; | ||
+ | } | ||
+ | .notebook_week img{ | ||
+ | margin-top: 30px; | ||
+ | } | ||
+ | |||
+ | .notebook_information_word1 { | ||
+ | margin-top: 40px; | ||
+ | display: none; | ||
+ | } | ||
+ | |||
+ | .notebook_information_button1 { | ||
+ | |||
+ | color: #ffffff; | ||
+ | background: #4e72b8; | ||
+ | width: 80px; | ||
+ | height: 40px; | ||
+ | border: 0px; | ||
+ | cursor: pointer; | ||
+ | border-radius: 10px; | ||
+ | font-size: 18px; | ||
+ | outline: none; | ||
+ | margin-top: 30px; | ||
+ | } | ||
+ | |||
+ | .notebook_information_button2 { | ||
+ | margin-top: 50px; | ||
+ | color: #ffffff; | ||
+ | background: #4e72b8; | ||
+ | width: 80px; | ||
+ | height: 40px; | ||
+ | border: 0px; | ||
+ | cursor: pointer; | ||
+ | border-radius: 10px; | ||
+ | font-size: 18px; | ||
+ | outline: none; | ||
+ | } | ||
+ | |||
+ | .notebook_information_word2 { | ||
+ | margin-top: 40px; | ||
+ | display: none; | ||
+ | } | ||
+ | |||
+ | .notebook_information_button3 { | ||
+ | margin-top: 50px; | ||
+ | color: #ffffff; | ||
+ | background: #4e72b8; | ||
+ | width: 80px; | ||
+ | height: 40px; | ||
+ | border: 0px; | ||
+ | cursor: pointer; | ||
+ | border-radius: 10px; | ||
+ | font-size: 18px; | ||
+ | outline: none; | ||
+ | } | ||
+ | |||
+ | .notebook_information_word3 { | ||
+ | margin-top: 40px; | ||
+ | display: none; | ||
+ | } | ||
+ | |||
+ | .notebook_information_button4 { | ||
+ | margin-top: 50px; | ||
+ | color: #ffffff; | ||
+ | background: #4e72b8; | ||
+ | width: 80px; | ||
+ | height: 40px; | ||
+ | border: 0px; | ||
+ | cursor: pointer; | ||
+ | border-radius: 10px; | ||
+ | font-size: 18px; | ||
+ | outline: none; | ||
+ | } | ||
+ | |||
+ | .notebook_information_word4 { | ||
+ | margin-top: 40px; | ||
+ | display: none; | ||
+ | } | ||
+ | |||
+ | .notebook_Month_head { | ||
+ | margin-top: -10px; | ||
+ | font-size: 25px; | ||
+ | } | ||
+ | </style> | ||
+ | </head> | ||
+ | |||
+ | <body> | ||
+ | <div class="nav"> | ||
+ | <div class="logo"> | ||
+ | <img class="logo_picture" src="https://static.igem.org/mediawiki/2018/f/f7/T--TJU_China--TJU_China.png"> | ||
+ | </div> | ||
+ | <ul style="margin-left:80px;" class="main"> | ||
+ | <li> | ||
+ | <div class="nav_logo_pic" style="width:24px;height:24px;margin-top:13px;"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/3/37/T--TJU_China--home.png"> | ||
+ | </div> | ||
+ | <a href="https://2018.igem.org/Team:TJU_China">Home</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <div class="nav_logo_pic"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/9/91/T--TJU_China--project.png"> | ||
+ | </div> | ||
+ | <a href="https://2018.igem.org/Team:TJU_China/Description"> | ||
+ | Project | ||
+ | </a> | ||
+ | <ul class="drop menu1"> | ||
+ | <li> | ||
+ | <a href="https://2018.igem.org/Team:TJU_China/Description">Description</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2018.igem.org/Team:TJU_China/Experiments">Experiments</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2018.igem.org/Team:TJU_China/Notebook">Notebook</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2018.igem.org/Team:TJU_China/Demonstrate">Demonstrate</a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | |||
+ | </li> | ||
+ | <li> | ||
+ | |||
+ | <div class="nav_logo_pic"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/0/0b/T--TJU_China--parts.png"> | ||
+ | </div> | ||
+ | <a href="https://2018.igem.org/Team:TJU_China/Parts"> | ||
+ | Parts | ||
+ | </a> | ||
+ | <ul class="drop menu2"> | ||
+ | <li> | ||
+ | <a href="https://2018.igem.org/Team:TJU_China/Parts#Parts_Overview">Parts Overview</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2018.igem.org/Team:TJU_China/Parts#Basic">Basic</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2018.igem.org/Team:TJU_China/Parts#Composite">Composite</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2018.igem.org/Team:TJU_China/Parts#Collection">Collection</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2018.igem.org/Team:TJU_China/Parts#Improve">Improve</a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <div class="nav_logo_pic"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/5/57/T--TJU_China--drylab.png"> | ||
+ | </div> | ||
+ | <a href="https://2018.igem.org/Team:TJU_China/Model">Model</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <div class="nav_logo_pic"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/e/ea/T--TJU_China--hp.png"> | ||
+ | </div> | ||
+ | <a href="https://2018.igem.org/Team:TJU_China/Human_Practices"> | ||
+ | HP | ||
+ | </a> | ||
+ | <ul class="drop menu3"> | ||
+ | <li> | ||
+ | <a href="https://2018.igem.org/Team:TJU_China/Human_Practices">Human Practices</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2018.igem.org/Team:TJU_China/Public_Engagement">Public Engagement</a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <div class="nav_logo_pic"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/0/01/T--TJU_China--team.png"> | ||
+ | </div> | ||
+ | <a href="https://2018.igem.org/Team:TJU_China/Team"> | ||
+ | Team | ||
+ | </a> | ||
+ | <ul class="drop menu7"> | ||
+ | <li style="list-style-type: none;"> | ||
+ | <a href="https://2018.igem.org/Team:TJU_China/Team">Members</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2018.igem.org/Team:TJU_China/Collaborations">Collaboration</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2018.igem.org/Team:TJU_China/Attributions">Attributions</a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | |||
+ | </ul> | ||
+ | |||
+ | </div> | ||
+ | <div style=" margin-top:28px;z-index:10; border-top: solid #4e72b8 2px;width: 100%; position: fixed;"></div> | ||
+ | <div class="notebook_month"> | ||
+ | <div class="notebook_month_p">Mar.</div> | ||
+ | <div class="notebook_month_p">Apr.</div> | ||
+ | <div class="notebook_month_p">May</div> | ||
+ | <div class="notebook_month_p">Jun.</div> | ||
+ | <div class="notebook_month_p">Jul.</div> | ||
+ | <div class="notebook_month_p">Aug.</div> | ||
+ | <div class="notebook_month_p">Sep.</div> | ||
+ | <div class="notebook_month_p">Oct.</div> | ||
+ | </div> | ||
+ | <div class="notebook_timeline"> | ||
+ | </div> | ||
+ | <div class="notebook_button_list"> | ||
+ | <button class="notebook_button1" id="notebook_btn1"></button> | ||
+ | <button class="notebook_button2" id="notebook_btn2"></button> | ||
+ | <button class="notebook_button3" id="notebook_btn3"></button> | ||
+ | <button class="notebook_button4" id="notebook_btn4"></button> | ||
+ | <button class="notebook_button5" id="notebook_btn5"></button> | ||
+ | <button class="notebook_button6" id="notebook_btn6"></button> | ||
+ | <button class="notebook_button7" id="notebook_btn7"></button> | ||
+ | <button class="notebook_button8" id="notebook_btn8"></button> | ||
+ | </div> | ||
+ | class="notebook_week_p" | ||
+ | <div class="notebook_information"> | ||
+ | <div id="notebook_March"> | ||
+ | <div class="notebook_Month_head">March</div> | ||
+ | <div class="notebook_week_p">Group1 get started at July.</div> | ||
+ | |||
+ | </div> | ||
+ | <div id="notebook_April"> | ||
+ | <div class="notebook_Month_head">April</div> | ||
+ | <div class="notebook_week_p">Group1 get started at July.</div> | ||
+ | |||
+ | </div> | ||
+ | <div id="notebook_May"> | ||
+ | <div class="notebook_Month_head">May</div> | ||
+ | <div class="notebook_week_p">Group1 get started at July.</div> | ||
+ | |||
+ | </div> | ||
+ | <div id="notebook_June"> | ||
+ | <div class="notebook_Month_head">June</div> | ||
+ | <div class="notebook_week_p">Group1 get started at July.</div> | ||
+ | |||
+ | </div> | ||
+ | <div id="notebook_July"> | ||
+ | <div class="notebook_Month_head">July</div> | ||
+ | <div class="notebook_week"> | ||
+ | <div> | ||
+ | <button id="7week2" class="notebook_information_button2">week2</button> | ||
+ | </div> | ||
+ | <div id="7notebook_week2" class="notebook_information_word2" style="display:none;"> | ||
+ | <div class="notebook_week_p">Activate smURFP glycerol.</div> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/8/88/T--TJU_China--g1.1.png"> | ||
+ | <div class="notebook_week_p"> | ||
+ | <b>Figure 1.</b> Incubate to activate the smURFP plasmid PCR amplification of smURFP fragments.</div> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/6/67/T--TJU_China--g1.2.png"> | ||
+ | <div class="notebook_week_p"> | ||
+ | <b>Figure 2.</b> The result of nucleic acid gel electrophoresis of smURFP after PCR. Lane M, Marker. | ||
+ | Lane1-8,smURFP. | ||
+ | <br>Gel Extraction purification of the PCR product. | ||
+ | <br>Find the arsenic ion plasmid inside the box and transform the plasmid into E.coli DH5α.</div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div> | ||
+ | <button id="7week3" class="notebook_information_button3">week3</button> | ||
+ | </div> | ||
+ | <div id="7notebook_week3" class="notebook_information_word3" style="display:none;"> | ||
+ | <div class="notebook_week_p">Transform the plasmid(Bba-J33201) inside the box. | ||
+ | <br>Amplification of PCR fragments. | ||
+ | <br>PCR amplification of arsenic ion loop.</div> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/2/2b/T--TJU_China--g1.3.png"> | ||
+ | <div class="notebook_week_p"> | ||
+ | <b>Figure 3.</b> The result of nucleic acid gel electrophoresis of Bba-J33201 after PCR. Lane M, Marker. | ||
+ | Lane 1-6,BBa-J33201.</div> | ||
+ | <div class="notebook_week_p">PCR amplification of ArsR promoter and ArsR protein fragment. | ||
+ | <br>Transform plasmid pKM586 into E.coli BL21. | ||
+ | <br>Activate and extract pKM586 plasmid.</div> | ||
+ | </div> | ||
+ | |||
+ | <div> | ||
+ | <button id="7week4" class="notebook_information_button4">week4</button> | ||
+ | </div> | ||
+ | <div id="7notebook_week4" class="notebook_information_word4" style="display:none;"> | ||
+ | <div class="notebook_week_p">Activate smURFP glycerol.</div> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/7/74/T--TJU_China--g1.4.png"> | ||
+ | <div class="notebook_week_p"> | ||
+ | <b>Figure 4.</b> The result of nucleic acid gel electrophoresis after overlapping of ArsR Promoter and | ||
+ | smURFP. Lane M, Marker. Lane 1-10, ArsR Promoter+smURFP</div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | <div id="notebook_August"> | ||
+ | <div class="notebook_Month_head">August</div> | ||
+ | <div class="notebook_week"> | ||
+ | <div> | ||
+ | <button id="8week1" class="notebook_information_button1">week1</button> | ||
+ | </div> | ||
+ | <div id="8notebook_week1" class="notebook_information_word1" style="display:none;"> | ||
+ | <div class="notebook_week_p">Double digestion of pKM586 plasmid.</div> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/7/74/T--TJU_China--g1.5.png"> | ||
+ | <div class="notebook_week_p"> | ||
+ | <b>Figure 5.</b> Double digestion of pKM586 with AatII and BamHI. Lane M, Marker. Lane 1,pKM586; Lane | ||
+ | 2-3, plasmid pKM586 after double enzyme digestion | ||
+ | |||
+ | <br>Overlap ArsR + Arsr Promoter,but failed. | ||
+ | |||
+ | <br>Overlap ArsR Promoter + smURFP, failed. | ||
+ | <br>Enzyme digestion of pwj66.</div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div> | ||
+ | <button id="8week2" class="notebook_information_button2">week2</button> | ||
+ | </div> | ||
+ | <div id="8notebook_week2" class="notebook_information_word2" style="display:none;"> | ||
+ | <div class="notebook_week_p">Enzyme digestion of pwj66. | ||
+ | <br>Connect pwj66+ TARGETDNA. | ||
+ | <br>Double digestion of pKM586. | ||
+ | <br>Overlap ArsR + Arsr Promoter. | ||
+ | <br>Overlap ArsR Promoter + smURFP. | ||
+ | <br>PCR of ParsR promoter ArsR protein fragment.</div> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/1/13/T--TJU_China--g1.6.png"> | ||
+ | <div class="notebook_week_p"> | ||
+ | <b>Figure 6.</b>LB media of pKM586 after transformed</div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div> | ||
+ | <button id="8week3" class="notebook_information_button3">week3</button> | ||
+ | </div> | ||
+ | <div id="8notebook_week3" class="notebook_information_word3" style="display:none;"> | ||
+ | <div class="notebook_week_p">Enzyme digestion of pwj66. | ||
+ | <br>Connect pwj66+ spacer. | ||
+ | <br>Double digestion pkm586. | ||
+ | <br>Overlap ArsR + Arsr Promoter. | ||
+ | <br>Overlap ArsR Promoter + smURFP.</div> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/6/64/T--TJU_China--g1.7.png"> | ||
+ | <div class="notebook_week_p"> | ||
+ | <b>Figure 7.</b>The result of nucleic acid gel electrophoresis after overlapping of ArsR Promoter and | ||
+ | smURFP. Lane M, Marker. Lane 1-7, ArsR Promoter+smURFP. PCR of ParsR promoter ArsR protein fragment.</div> | ||
+ | </div> | ||
+ | <div> | ||
+ | <button id="8week4" class="notebook_information_button4">week4</button> | ||
+ | </div> | ||
+ | <div id="8notebook_week4" class="notebook_information_word4" style="display:none;"> | ||
+ | <div class="notebook_week_p">Anneal two J23104 oligo chains. | ||
+ | <br>PCR amplification of Bba-J23104 fragments. . | ||
+ | <br>Overlap J23104 + ArsR protein fragment. | ||
+ | <br>Overlap smURFP + ArsR promoter.</div> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/9/92/T--TJU_China--g1.8.png"> | ||
+ | <div class="notebook_week_p"> | ||
+ | <b>Figure 8.</b> The result of nucleic acid gel electrophoresis after overlapping of ArsR Promoter and | ||
+ | smURFP. Lane M, Marker. Lane 1, smURFP. Lane 2-4, ArsR Promoter+smURFP.</div> | ||
+ | <div class="notebook_week_p">Overlapping the two overlapped fragments. | ||
+ | <br>Gel Extraction purification. | ||
+ | <br>Double enzyme digestion of the entire arsenic loop. | ||
+ | <br>Double digestion of pKM586.</div> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/4/47/T--TJU_China--g1.9.png"> | ||
+ | <div class="notebook_week_p"> | ||
+ | <b>Figure 9.</b>Double digestion of pKM586 with AatII and BamHI. Lane M, Marker. Lane 1,Plasmid pKM586. | ||
+ | Lane 2, Plasmid pKM586 after double enzyme digestion | ||
+ | <br>Connection the two fragments after enzyme digestion | ||
+ | <br>Transform the product into E.coli DH5α</div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <div id="notebook_September"> | ||
+ | <div class="notebook_Month_head">September</div> | ||
+ | <div class="notebook_week"> | ||
+ | <div> | ||
+ | <button id="9week1" class="notebook_information_button1">week1</button> | ||
+ | </div> | ||
+ | <div id="9notebook_week1" class="notebook_information_word1" style="display:none;"> | ||
+ | <div class="notebook_week_p">We did a full fragment overlap of the arsenic loop and the result failed. Double digestion of pKM586 | ||
+ | vector, plasmid extraction results and double enzyme digestion results are not ideal</div> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/3/3e/T--TJU_China--g1.10.png"> | ||
+ | <div class="notebook_week_p"> | ||
+ | <b>Figure 10.</b>Double digestion of pKM586 with AatII and BamHI. Lane M, Marker. Lane 1, pKM586; Lane | ||
+ | 2-3, plasmid pKM586 after double enzyme digestion</div> | ||
+ | </div> | ||
+ | <div> | ||
+ | <button id="9week2" class="notebook_information_button2">week2</button> | ||
+ | </div> | ||
+ | <div id="9notebook_week2" class="notebook_information_word2" style="display:none;"> | ||
+ | <div class="notebook_week_p">We modified the arsenic loop full-segment overlap system and the PCR program, and the results were successful.</div> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/7/79/T--TJU_China--g1.11.png"> | ||
+ | <div class="notebook_week_p"> | ||
+ | <b>Figure 11.</b>The result of nucleic acid gel electrophoresis after overlapping the whole arsenic | ||
+ | loop. Lane M, Marker. Lane1-7 the whole arsenic loop.</div> | ||
+ | <div class="notebook_week_p">Reconstruction of pKM586 original plasmid, the quality of plasmid extraction is not high</div> | ||
+ | </div> | ||
+ | <div> | ||
+ | <button id="9week3" class="notebook_information_button3">week3</button> | ||
+ | </div> | ||
+ | <div id="9notebook_week3" class="notebook_information_word3" style="display:none;"> | ||
+ | <div class="notebook_week_p">Try pKM586 step-by-step double enzyme digestion pre-experiment, resulting in significant improvement | ||
+ | in cutting efficiency. The arsenic loop complete fragment double digestion and pKM586 double digestion | ||
+ | product gel recovery, as well as ligation transformation.</div> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/8/83/T--TJU_China--g1.12.png"> | ||
+ | <div class="notebook_week_p"> | ||
+ | <b>Figure 12.</b>the arsenic loop after transformed into DH5α</div> | ||
+ | </div> | ||
+ | <div> | ||
+ | <button id="9week4" class="notebook_information_button4">week4</button> | ||
+ | </div> | ||
+ | <div id="9notebook_week4" class="notebook_information_word4" style="display:none;"> | ||
+ | <div class="notebook_week_p">The plasmid was extracted and verified by double enzyme digestion. The result was successful.</div> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/f/f0/T--TJU_China--g1.13.png"> | ||
+ | <div class="notebook_week_p"> | ||
+ | <b>Figure 13.</b>Double digestion to verify the ligation product. Lane M, Marker. Lane 1, Plasmid pKM586. | ||
+ | Lane 2, Plasmid pKM586 single digestion with BamHI. Lane 3, Plasmid pKM586 double digestion with | ||
+ | AatII and BamHI. Lane 4, Plasmid ArS. Lane 5, Plasmid ArS single digestion with BamHI. Lane 6, Plasmid | ||
+ | ArS double gigestion with AatII and BamHI. Lane 7, Plasmid ArS. Lane 8, Plasmid ArS single digestion | ||
+ | with BamHI. Lane 9, Plasmid ArS double digestion with AatII and BamHI.</div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <div id="notebook_October"> | ||
+ | <div class="notebook_Month_head">October</div> | ||
+ | <div class="notebook_week"> | ||
+ | <div> | ||
+ | <button id="10week1" class="notebook_information_button1">week1</button> | ||
+ | </div> | ||
+ | <div id="10notebook_week1" class="notebook_information_word1" style="display:none;"> | ||
+ | <div class="notebook_week_p">Transform the successfully constructed plasmid into E. coli BL21, pick a single colony, incubate the | ||
+ | tube for the first arsenic ion delivery experiment, but the result shows no fluorescence.</div> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/9/94/T--TJU_China--g1.14.png"> | ||
+ | <div class="notebook_week_p"> | ||
+ | <b>Figure 14.</b>the arsenic loop after transformed into BL21</div> | ||
+ | </div> | ||
+ | <div> | ||
+ | <button id="10week2" class="notebook_information_button2">week2</button> | ||
+ | </div> | ||
+ | <div id="10notebook_week2" class="notebook_information_word2" style="display:none;"> | ||
+ | <div class="notebook_week_p">After the successful construction of the plasmid, we began to try to test whether the transformed E. | ||
+ | coli can express fluorescence after adding arsenic ions. We chose to culture the tube for 6 hours | ||
+ | and then add different concentrations of arsenic ions for detection. For the second time, we failed | ||
+ | again. | ||
+ | <br>And we continue to try to change the condition to make it express fluorescence.</div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
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+ | |||
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+ | </body> | ||
+ | <!-- | ||
+ | <div class="notebook_week_p"></div> | ||
+ | <img src=""> | ||
+ | <div class="notebook_week_p"><b>Figure .</b></div> | ||
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Latest revision as of 12:11, 17 October 2018
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