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− | The part codes for the enzyme catechol-2,3-dioxygenase which cleaves the oxidative ring of catechol to form 2-hydroxymuconate semialdehyde. It also contains a 6X His-tag which is a useful marker for purification and identification of the protein of interest. | + | The part codes for the enzyme catechol-2,3-dioxygenase which cleaves the oxidative ring of catechol to form 2-hydroxymuconate semialdehyde. <br> |
+ | It also contains a 6X His-tag which is a useful marker for purification and identification of the protein of interest. | ||
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− | <img src="https://static.igem.org/mediawiki/2018/0/09/T--Ruia-Mumbai--XylE3D.png"> | + | <img src="https://static.igem.org/mediawiki/2018/0/09/T--Ruia-Mumbai--XylE3D.png" height="100px" width="100px"> |
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Revision as of 14:47, 17 October 2018
Basic Parts:BBa_K2749017
The part codes for the enzyme catechol-2,3-dioxygenase which cleaves the oxidative ring of catechol to form 2-hydroxymuconate semialdehyde.
It also contains a 6X His-tag which is a useful marker for purification and identification of the protein of interest.
It also contains a 6X His-tag which is a useful marker for purification and identification of the protein of interest.
Structure:
Fig1: 3D-Structure of enzyme catechol-2,3-dioxygenase
Fig2: Construct of the enzyme module