Difference between revisions of "Team:Newcastle/Software"

Line 69: Line 69:
 
     </section> <!-- end s-services -->
 
     </section> <!-- end s-services -->
  
 +
<section id='team' class="s-services">
 +
 +
 +
        <div class="row section-header has-bottom-sep" data-aos="fade-up">
 +
                <div class="col-full">
 +
                            <br>
 +
<br>
 +
<br>
 +
<br>
 +
<h3 class="subhead"></h3>
 +
                <h1 class="display-2">References & Attributions</h1>
 +
            </div>
 +
 +
        </div>
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
<button class="collapsible">Click for References</button>
 +
<div class="content">
 +
              <div class="row about-desc" data-aos="fade-up">
 +
                <div class="col-full">
 +
 +
<p class="about-para"><font size="2"><strong>Attributions: Umar Farooq
 +
</strong><font></p>
 +
 +
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</section>
  
 
      
 
      
Line 120: Line 160:
 
     <script type="text/javascript" src="https://2018.igem.org/Template:Newcastle/JSmain?
 
     <script type="text/javascript" src="https://2018.igem.org/Template:Newcastle/JSmain?
 
     action=raw&ctype=text/javascript"></script>  
 
     action=raw&ctype=text/javascript"></script>  
 +
 +
    <script>
 +
var coll = document.getElementsByClassName("collapsible");
 +
var i;
 +
 +
for (i = 0; i < coll.length; i++) {
 +
  coll[i].addEventListener("click", function() {
 +
    this.classList.toggle("active");
 +
    var content = this.nextElementSibling;
 +
    if (content.style.maxHeight){
 +
      content.style.maxHeight = null;
 +
    } else {
 +
      content.style.maxHeight = content.scrollHeight + "px";
 +
    }
 +
  });
 +
}
 +
</script>
 
</body>
 
</body>
 
</html>
 
</html>
 
{{Newcastle/footer}}
 
{{Newcastle/footer}}

Revision as of 17:03, 17 October 2018

Alternative Roots/Software

Alternative Roots

Software

OpenTrons NH-1

Overview

A significant proportion of the Alternative Roots project was based on software, whether that was for automation of experiments via the OpenTrons OT-2 or small-scale plant growth via our NH-1. Both systems were carefully programmed by our team for efficiency and reliability.

Newcastle were fortunate to win an OpenTrons OT-2 liquid-handling robot in 2018. The OT-2 is programmed in Python and is equipped with two pipettes capable of pipetting between 1 and 300 µL as well as a temperature module, capable of maintaining temperatures between 0-100 °C. We have created and deposited code that allows OT-2 users to automate small-scale (100 µL) heat-shock transformations of E. coli. The value of this code was demonstrated when it was used to perform a statistical ‘Definitive Screening Design’ screen to examine the impact that transformation buffers have on transformation efficiency. As a result, we identified a new buffer composition with 10-fold improved transformation efficiency. Perhaps more importantly, the combination of the code and the robot resulted in greater reliability than the manual protocol.







References & Attributions

Attributions: Umar Farooq