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<div class="title_2"><p>Meet all deliverables on the Competition Deliverables page, except those that specifically mention parts.</p></div> | <div class="title_2"><p>Meet all deliverables on the Competition Deliverables page, except those that specifically mention parts.</p></div> | ||
| − | <div class="title_1"><p>3. | + | <div class="title_1"><p>3. Attributions</p></div> |
<div class="title_2"><p>Create a page on your team wiki with clear attribution of each aspect of your project.</p></div> | <div class="title_2"><p>Create a page on your team wiki with clear attribution of each aspect of your project.</p></div> | ||
<div class="text"> | <div class="text"> | ||
1. Integrated Human Practices
Expand on your silver medal activity by demonstrating how you have integrated the investigated issues into the design and/or execution of your project.
The establishment of our system was inspired by the urgent need to figure out the solution to acidification problem. We visited the Agricultural Improvement Station and Professor Yang, a Chung Hsing University professor to find out just how big of a problem microbial imbalance is. They gave us tips on how to perform our experiments and meet the needs of the public.
(Human Practice)
2. Improve a previous part or project
Improve the function of an existing iGEM project and display your achievement on your wiki.
We improved the sequence of 2016 NCTU_Formosa team did before. We linked Sf1a and Lectin with GS linker to increase the folding efficiency of these two protein. Compared the result with 2016 NCTU_Formosa team, the plant protecting effect of our improvement part was more obvious, which larvae consumed less leaves areas than previous part. Moreover, larva also has abnormal movement after consuming leaf coated with the improved protein.
(Improvement)
3. Model your project
Convince the judges that your project's design and/or implementation is based on insight you have gained from modeling.
We improved the sequence NCTU_Formosa team did before. Before the sequence PE4, we add three tags (Trx-Tag, His-Tag, S-Tag) and use this biobrick to express protein. We use the minimum inhibitory concentration test to verify its function.
(Dry Lab)
4. Demonstrate your work
Convince the judges that your project works.
We’ve built Microbiota prediction model, Peptide prediction model, Growth model, and Productivity model. By these models, we can achieve precise and AI agriculture.
(Demonstration)
1. Validated Part / Validated Contribution
Convince the judges that something you created performs its intended function. Provide thorough documentation of this validation on your team wiki.
We have created a smart agriculture system that predicts an ideal amount of subtilis and other key bacteria for healthy soil and plant growth. Also, our feedback system can precisely predict how much bacteriocin is needed to produce an ideal balanced microbiota and bacteriocin performed as expect. Though NGS data analysis, we see whether our model and bacteriocin worked.
(Parts)
2. Collaboration
Convince the judges you have significantly worked with any other registered iGEM team in a meaningful way.
We worked in collaboration with NTHU_Formosa. We confirm the parts of NTHU_Formosa team. We did DNA electrophoresis to confirm the size of inserted vectors which are essential in the process of emitting the light. Also, we share the basic concept of designing websites and make them more familiar with web languages. For NTHU_Formosa team, they ran an SDS-PAGE of our bacteriocins to help us check if our constructs were correctly expressed.
(Collaboration)
3. Human Practices
Convince the judges you have thought carefully and creatively about whether your work is safe, responsible and good for the world.
To make our project more complete, we asked for many suggestions of professors and government agricultural improvement institute to learn more about the dosage of fertilizer farmer used. Also, we explore the possibilities of our solutions and the problems we may face.
(Human Practice)
1. Register and attend
Register for iGEM, have a great summer, and attend the Giant Jamboree.
2. Deliverables
Meet all deliverables on the Competition Deliverables page, except those that specifically mention parts.
3. Attributions
Create a page on your team wiki with clear attribution of each aspect of your project.
4. Characterization / Contribution
Successfully complete the InterLab Measurement Study. This means you have met all requirements of the InterLab Measurement Study, including acceptance of data.
The purpose of InterLab experiment is to standardize measurement techniques to make the data from synthetic biology laboratories around the world easier to compare with. This year, we focus on converting the fluorescence readings to absolute cell counts instead of relying on OD measurements since OD measurements usually vary from lab to lab. We calibrate and measure the value to complete the experiment.
(InterLab)
