Difference between revisions of "Team:METU HS Ankara"

 
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<br /><br /><strong>The Combination of FucO and GSH Stimulates Bioethanol Production from Lignocellulosic Biomass</strong> <br /><br />
 
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As energy resources get scarce, bioethanol production from lignocellulosic waste looks like a great alternative in terms of high energy yield and eco-friendliness. Due to their highly complex and rigid structure, lignocellulosic wastes need to be pretreated before they can be fermented. The process causes toxic byproducts such as furfural and 5-Hydroxymethylfurfural that inhibit the ethanol production and growth rate of bacteria, E. coli ethanologenic strain KO11. By integrating GSH and FucO genes into KO11 bacteria, we aim to enhance bioethanol production. Since furfural and HMF act as thiol-reactive electrophiles, cellular glutathione levels get depleted in their presence, leading to the accumulation of reactive oxygen species. Thus, overexpression of GSH increases cellular growth rates and lifespan. On the other hand, the expression of fucO results in the formation of NADH dependent furfural oxidoreductase which degrades furfural into furfuryl alcohol resulting in a higher rate of growth and ethanol fermentation.
 
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<h1> Welcome to iGEM 2018! </h1>
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            <source src="https://static.igem.org/mediawiki/2018/b/b0/T--METU_HS_Ankara--banner.mp4" type="video/mp4">
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<img src="http://placehold.it/1080x320/c4baba/e4dede">
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<h3>Before you start</h3>
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<p> Please read the following pages:</p>
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<ul>
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<li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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                <span class="text-capitalize">Support renewable energy</span>
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                <small class="ct-u-colorMotive ct-fw-300">Renew earth’s future with BIO-E</small>
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            </h2>
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                            problem
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                        <p style="text-align: center">
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                            The use of non-renewable energy resources ,
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                            by greenhouse gas emissions lead to environmental
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                            problems and also put great strains on countries’ budgets.
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                        </p>
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                    </div>
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                            vision
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                        </h4>
  
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                        <p style="text-align: center">
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                            As we sought an alternative energy source,
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                            renewable, sustainable and one that will outrun fossil
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                            fuels our research led us to biofuels.
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                        </p>
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                            solution
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                        </h4>
  
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                        <p style="text-align: center">
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                            Finally, we made use of bioethanol which is a
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                            renewable and sustainable green energy source that
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                            could be obtained from agricultural waste.
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                        </p>
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<h3> Styling your wiki </h3>
 
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
 
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
 
  
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                            What We Did:
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                    <p>
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                        We investigated ways to improve bioethanol production since it's a non-toxic, biodegradable and cost friendly alternative
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                        to fossil fuels being about 20-30% more efficient and its carbon footprint being far smaller.
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                    </p>
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                    <p>
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                        Doing so, we came across different bioenergy generations; first, second and third. We focused on second generation; energy
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                        from lignocellulosic biomass given that it's the most abundant, renewable and thus sustainable.
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                    </p>
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                    <p>
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                        In the light of our research, we developed a second generation bioethanol production system aiming to decrease the economic
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                        and environmental consequences of energy production. Our system will increase the efficiency of ethanol production, ultimately
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                        its sustainability using the ethanologenic <i>E.coli</i> strain KO11 and two genes; FucO and GSH.
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                    </p>
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                    <a href="https://2018.igem.org/Team:METU_HS_Ankara/Description" class="btn btn-primary ct-btn--rounded text-uppercase">Project Description</a>
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                    <a href="https://2018.igem.org/Team:METU_HS_Ankara/Demonstrate" class="btn ct-btn--black ct-btn--rounded text-uppercase">Demonstration</a>
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                            Fun facts.
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                        </h3>
  
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                        <span style="color: #FFF; font-size: 15px">
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                            iGEM is hard work and all, but never forget; there is no project without a little fun
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                            with your teammates.
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                        </span>
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                        <!--<a href="services.html" class="btn btn-primary ct-btn--rounded text-uppercase">Services</a>
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                        <a href="portfolio-masonry.html" class="btn btn-default ct-btn--rounded text-uppercase">Portfolio</a>-->
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                                      data-ct-to="324" data-ct-speed="5000">0</span>
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                                <h5 class="ct-counterBox-title text-uppercase ct-fw-700">days spent<br>working</h5>
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                                <span class="ct-counterBox-icon"><i class="fas fa-briefcase"></i> </span>
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                                      data-ct-to="2375" data-ct-speed="4000">0</span>
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                                <h5 class="ct-counterBox-title text-uppercase ct-fw-700">coffees/teas<br>drunk</h5>
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                                <span class="ct-counterBox-icon"><i class="fas fa-coffee"></i> </span>
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                                <span class="ct-counterBox-number ct-u-colorMotive ct-fs-i"><img src="https://static.igem.org/mediawiki/2018/4/40/T--METU_HS_Ankara--infinity.jpg" /></span>
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                                <h5 class="ct-counterBox-title text-uppercase ct-fw-700">issues<br>read</h5>
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                                <span class="ct-counterBox-icon"><i class="fab fa-readme"></i> </span>
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            <h2 class="ct-fw-600">
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                <span class="text-capitalize">beyond the lab bench</span>
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                <small class="ct-u-colorMotive ct-fw-300">
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                    We worked with many experts
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                </small>
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            </h2>
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                <span class="ct-pageSectionHeader-rightContent ct-u-colorMotive ct-fs-i ct-u-arapey">
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                    <!--02-->
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                                        Collaboration
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                                    </a>
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                                </h4>
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                                    <p>
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                                        Since collaborating is an essential part of iGEM, encouraging teams to
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                                        overcome problems, we have interacted with various teams from all around
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                                        the world. To see what we’ve achieved together, please proceed to the
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                                        <a style="color: #68bd45" href="https://2018.igem.org/Team:METU_HS_Ankara/Collaborations">collaborations</a> page.
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                                    </p>
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                                        Attributions
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                                    </a>
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                                    <p>
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                                        Since science is a collective effort, one can never pursue great things
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                                        alone and without taking help from people who are experts in the specific
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                                        subjects. Not to mention the nature of iGEM projects requiring us to be
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                                        well-rounded, it's fair to say it's next to impossible to do it alone.
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                                        See the people and their contributions to our project on the page
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                                        <a style="color: #68bd45" href="https://2018.igem.org/Team:METU_HS_Ankara/Attributions">attributions</a>.
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                                    </p>
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                                      class="collapsed">
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                                        Human Practices
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                                    </a>
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                                </h4>
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                                    <p>
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                                        Beyond the lab, we were there to be a part of the society. During the making
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                                        of our project, we benefited from the public idea as much as we benefited
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                                        from any protocol or article. We strove to integrate what we have learned from
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                                        others to our project in order to make the best use of Bio-E, since we wanted
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                                        the whole world to make use of our fermentation process in the future. You can see our human practices on the
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                                        <a style="color: #68bd45" href="https://2018.igem.org/Team:METU_HS_Ankara/Human_Practices"> Human Practices </a> and
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                                        <a style="color: #68bd45" href="https://2018.igem.org/Team:METU_HS_Ankara/Public_Engagement"> Education and Engagement </a> pages.
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                                    </p>
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                                </div>
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                            </div>
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                        Contrary to popular belief, iGEM is much more than what meets the eye. It means to be
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                        conscious, it means to have a community, it means entrepreneurship. Just like all the
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                        iGEM teams, our experience leads us to learn that science is truly a collective endeavor.
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                        Here is our interactions with peers, experts, and the public.
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                        <h3 class="text-capitalize ct-fw-600">
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                            The Team.
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                            Every member of this team has spared tremendous efforts and endless work hours to make
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<h3> Uploading pictures and files </h3>
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                            our project come to life. The team is not only about working together but like a family.
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<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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                        <br><br>
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                        <a href="https://2018.igem.org/Team:METU_HS_Ankara/Team" class="btn btn-primary ct-btn--rounded text-uppercase" style="color: #FFF">Team</a>
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                        <a href="https://2018.igem.org/Team:METU_HS_Ankara/Collaborations" class="btn btn-default ct-btn--rounded text-uppercase">Collaborations</a>
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                            <li><img src="https://static.igem.org/mediawiki/2018/1/12/T--METU_HS_Ankara--t01.jpg" alt="METU HS"></li>
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                            <li><img src="https://static.igem.org/mediawiki/2018/6/60/T--METU_HS_Ankara--t02.jpg" alt="METU HS"></li>
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                            <li><img src="https://static.igem.org/mediawiki/2018/1/1b/T--METU_HS_Ankara--t03.jpg" alt="METU HS"></li>
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                            <li><img src="https://static.igem.org/mediawiki/2018/3/3b/T--METU_HS_Ankara--t04.jpg" alt="METU HS"></li>
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                            <li><img src="https://static.igem.org/mediawiki/2018/e/ef/T--METU_HS_Ankara--t05.jpg" alt="METU HS"></li>
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                            <li><img src="https://static.igem.org/mediawiki/2018/9/96/T--METU_HS_Ankara--t06.jpg" alt="METU HS"></li>
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                            <li><img src="https://static.igem.org/mediawiki/2018/4/49/T--METU_HS_Ankara--t07.jpg" alt="METU HS"></li>
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                        </ul>
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                    </div>
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                </div>
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                <h2 class="ct-fw-600">
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                    <span class="text-capitalize">Our Project</span>
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{{METU_HS_Ankara/footer}}

Latest revision as of 20:24, 17 October 2018

METU HS IGEM

METUHSIGEM_LOGO

Support renewable energy Renew earth’s future with BIO-E

problem

The use of non-renewable energy resources , by greenhouse gas emissions lead to environmental problems and also put great strains on countries’ budgets.

vision

As we sought an alternative energy source, renewable, sustainable and one that will outrun fossil fuels our research led us to biofuels.

solution

Finally, we made use of bioethanol which is a renewable and sustainable green energy source that could be obtained from agricultural waste.

What We Did:

We investigated ways to improve bioethanol production since it's a non-toxic, biodegradable and cost friendly alternative to fossil fuels being about 20-30% more efficient and its carbon footprint being far smaller.

Doing so, we came across different bioenergy generations; first, second and third. We focused on second generation; energy from lignocellulosic biomass given that it's the most abundant, renewable and thus sustainable.

In the light of our research, we developed a second generation bioethanol production system aiming to decrease the economic and environmental consequences of energy production. Our system will increase the efficiency of ethanol production, ultimately its sustainability using the ethanologenic E.coli strain KO11 and two genes; FucO and GSH.

Project Description Demonstration

Fun facts.

iGEM is hard work and all, but never forget; there is no project without a little fun with your teammates.
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working
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coffees/teas
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beyond the lab bench We worked with many experts

Collaboration

Since collaborating is an essential part of iGEM, encouraging teams to overcome problems, we have interacted with various teams from all around the world. To see what we’ve achieved together, please proceed to the collaborations page.

Since science is a collective effort, one can never pursue great things alone and without taking help from people who are experts in the specific subjects. Not to mention the nature of iGEM projects requiring us to be well-rounded, it's fair to say it's next to impossible to do it alone. See the people and their contributions to our project on the page attributions.

Beyond the lab, we were there to be a part of the society. During the making of our project, we benefited from the public idea as much as we benefited from any protocol or article. We strove to integrate what we have learned from others to our project in order to make the best use of Bio-E, since we wanted the whole world to make use of our fermentation process in the future. You can see our human practices on the Human Practices and Education and Engagement pages.

Contrary to popular belief, iGEM is much more than what meets the eye. It means to be conscious, it means to have a community, it means entrepreneurship. Just like all the iGEM teams, our experience leads us to learn that science is truly a collective endeavor. Here is our interactions with peers, experts, and the public.

The Team.

Every member of this team has spared tremendous efforts and endless work hours to make our project come to life. The team is not only about working together but like a family.

Team Collaborations
  • METU HS
  • METU HS
  • METU HS
  • METU HS
  • METU HS
  • METU HS
  • METU HS

Our Project

>