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</head> | </head> | ||
− | <body> | + | <body data-spy="scroll" data-target=".navbar-example"> |
<!--Page_Content--> | <!--Page_Content--> | ||
<div class="container content"> | <div class="container content"> | ||
− | + | <div class="headstyle"> | |
+ | <h1 class="head">pH Meter</h1> | ||
+ | </div> | ||
+ | <div class="righttitle"> | ||
+ | <h6 class="subtitle"></h6> | ||
+ | </div> | ||
<div class="navbar-example"> | <div class="navbar-example"> | ||
<div class="row"> | <div class="row"> | ||
<div class="col-2 side"> | <div class="col-2 side"> | ||
<div id="sidelist" class="list-group"> | <div id="sidelist" class="list-group"> | ||
− | <a class="list-group-item list-group-item-action" href="# | + | <a class="list-group-item list-group-item-action" href="#Motivation">Motivation</a> |
− | <a class="list-group-item list-group-item-action" href="# | + | <a class="list-group-item list-group-item-action" href="#Experiments">Experiments</a> |
− | <a class="list-group-item list-group-item-action" href="# | + | <a class="list-group-item list-group-item-action" href="#Wiring">Wiring</a> |
− | <a class="list-group-item list-group-item-action" href="# | + | <a class="list-group-item list-group-item-action" href="#Reference">Reference</a> |
<a class="list-group-item list-group-item-action" href="#"><i class="fa fa-arrow-up fa-1x" | <a class="list-group-item list-group-item-action" href="#"><i class="fa fa-arrow-up fa-1x" | ||
aria-hidden="true"></i></a> | aria-hidden="true"></i></a> | ||
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<div data-spy="scroll" data-target="#sidelist" data-offset="0" class="scrollspy-example"> | <div data-spy="scroll" data-target="#sidelist" data-offset="0" class="scrollspy-example"> | ||
<div class="container"> | <div class="container"> | ||
− | + | <img class="contentimg col-12" src="https://static.igem.org/mediawiki/2018/f/f2/T--NCKU_Tainan--pH_sensor.jpg"> | |
− | + | <p class="pcontent"> | |
− | + | About this section, we are showing how to use the pH meter in arduino. | |
− | + | </p> | |
− | + | <div id="Motivation"> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
<h3>Motivation</h3></br> | <h3>Motivation</h3></br> | ||
<div id="pt"> | <div id="pt"> | ||
<p class="pcontent"> | <p class="pcontent"> | ||
− | Because E. coli is sensitive to pH value, and according to the experiment | + | Because <I>E. coli</I> is sensitive to pH value, and according to the experiment |
of | of | ||
− | the pH sensor by WET members. We know that E. coli can’t grow below pH | + | the pH sensor by <a href="https://2018.igem.org/Team:NCKU_Tainan/Improve#Experiment_Result" |
+ | style="color: #28ff28;">WET</a> members. We know that <I>E. coli</I> can’t grow | ||
+ | below pH | ||
value of | value of | ||
6, and | 6, and | ||
− | generally grow the best | + | generally grow the best at pH 7. |
</p> | </p> | ||
</div> | </div> | ||
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− | <div id=" | + | <div id="Experiments"> |
− | + | ||
<h3>Experiments</h3></br> | <h3>Experiments</h3></br> | ||
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<ul> | <ul> | ||
− | <li>Because the buffer solution wouldn’t change too much in the | + | <li class="none">Because the buffer solution wouldn’t change too much in the |
environment, we used the buffer solution to calibrate the pH meter to | environment, we used the buffer solution to calibrate the pH meter to | ||
− | ensure that the deviation would the same every time.</li></br> | + | ensure that the deviation would be the same every time.</li></br> |
</ul> | </ul> | ||
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<ol type="a"> | <ol type="a"> | ||
− | <li>Insert pH meter into | + | <li>Insert pH meter into buffer solution of pH 7, wait for 1 min, it |
will achieve a stable | will achieve a stable | ||
value.</li></br> | value.</li></br> | ||
<li>Minus the value with pH 7, and it will get the offset value. For | <li>Minus the value with pH 7, and it will get the offset value. For | ||
− | instance, 7-7.09=-0.09.</li | + | instance, 7-7.09=-0.09.</li> |
<div id="centerimg" class="pic1"> | <div id="centerimg" class="pic1"> | ||
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</br> | </br> | ||
− | <li>Write the offset value into code, upload the code into Arduino | + | <li>Write the offset value into code, and then upload the code into Arduino |
− | again.</li | + | again.</li> |
− | <div id="centerimg" class="pic2"> | + | <div id="centerimg" class="pic2 knob"> |
<img class="oneimg" src="https://static.igem.org/mediawiki/2018/2/2d/T--NCKU_Tainan--home_04-ph_meter-3.jpg"> | <img class="oneimg" src="https://static.igem.org/mediawiki/2018/2/2d/T--NCKU_Tainan--home_04-ph_meter-3.jpg"> | ||
</div> | </div> | ||
</br> | </br> | ||
− | <li>After rinsing the pH meter, insert it into pH 4 buffer | + | <li>After rinsing the pH meter, we will insert it into pH 4 buffer |
solution.</li></br> | solution.</li></br> | ||
− | <li>If it is found to be too different from the error of pH | + | <li>If it is found to be too different from the error of pH 4, you |
should adjust the knob. | should adjust the knob. | ||
− | After inserting pH | + | After inserting pH 4, it is found that the error with pH 4 is very |
− | large.</li | + | large.</li> |
<div id="centerimg" class="pic3"> | <div id="centerimg" class="pic3"> | ||
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<div id="centerimg" class="pic4"> | <div id="centerimg" class="pic4"> | ||
− | <img class="oneimg" src=""> | + | <img class="oneimg" src="https://static.igem.org/mediawiki/2018/e/ea/T--NCKU_Tainan--home_manual_pH_%E5%A2%9E%E4%B8%80.jpg"> |
</div> | </div> | ||
− | + | </br> | |
<li>Finally, it can be found that the measured pH is 4.</li></br> | <li>Finally, it can be found that the measured pH is 4.</li></br> | ||
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</ol> | </ol> | ||
− | + | </br> | |
<li>Formula calculation</li></br> | <li>Formula calculation</li></br> | ||
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<li>Purpose</li></br> | <li>Purpose</li></br> | ||
<ul> | <ul> | ||
− | <li>We assume that pH 4 to 7 is linear, so we want to verify | + | <li class="none">We assume that pH 4 to 7 is linear, so we want to verify |
whether it is linear between pH | whether it is linear between pH | ||
4 to 7. By measuring the solution of pH 4.7, compare its | 4 to 7. By measuring the solution of pH 4.7, compare its | ||
Line 147: | Line 147: | ||
<ul> | <ul> | ||
− | <li> | + | <li class="none">Mix a solution with pH=4.7 and measure with a calibrated pH |
meter.</li></br> | meter.</li></br> | ||
</ul> | </ul> | ||
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<ul> | <ul> | ||
− | <li>After inserting the solution with pH=4.7, we found that the | + | <li class="none">After inserting the solution with pH=4.7, we found that the |
value was stable at pH 4.83 | value was stable at pH 4.83 | ||
with an error of about 0.13, which roughly met the error of | with an error of about 0.13, which roughly met the error of | ||
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</div> | </div> | ||
− | + | </br> | |
− | + | <li>Materials used</li></br> | |
<ol> | <ol> | ||
<li>Arduino UNO</li></br> | <li>Arduino UNO</li></br> | ||
<li>pH sensor</li> | <li>pH sensor</li> | ||
− | + | <ol type="a"> | |
− | + | <li>Module Power : 5.00V</li> | |
− | + | <li>Measuring Range:0-14pH</li> | |
− | + | <li>Measuring Temperature :0-60 ℃</li> | |
− | + | <li>Accuracy : ± 0.1pH (25 ℃)</li> | |
− | + | </ol> | |
</br> | </br> | ||
<li>pH buffer solution</li></br> | <li>pH buffer solution</li></br> | ||
− | <li><a href="https://github.com/vicky87106/2018iGEM_NCKU-Tainan style="color: #28ff28;">Arduino code</a></li></br> | + | <li><a href="https://github.com/vicky87106/2018iGEM_NCKU-Tainan" style="color: #28ff28;">Arduino |
+ | code</a></li></br> | ||
</ol> | </ol> | ||
+ | </ol> | ||
+ | |||
− | |||
− | |||
</ul> | </ul> | ||
</div> | </div> | ||
− | <div id=" | + | <div id="Wiring"> |
− | + | ||
− | + | ||
− | + | ||
<h3>Wiring</h3></br> | <h3>Wiring</h3></br> | ||
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− | <div id=" | + | <div id="Reference"> |
− | + | ||
− | + | ||
− | + | ||
<h3>Reference</h3></br> | <h3>Reference</h3></br> | ||
<ol> | <ol> | ||
− | <li>Boywhy Chen.(2016).[Arduino] PH meter. Retrieved from</br> | + | <li class="smallp">Boywhy Chen.(2016).[Arduino] PH meter. Retrieved from</br> |
http://boywhy.blogspot.com/2016/07/arduino-ph-meter.html(Augest 28,2018)</li> | http://boywhy.blogspot.com/2016/07/arduino-ph-meter.html(Augest 28,2018)</li> | ||
</ol> | </ol> | ||
− | + | </br></br></br></br> | |
</div> | </div> | ||
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</div> | </div> | ||
+ | <style> | ||
+ | .list-group-item { | ||
+ | margin: 10px 0 !important; | ||
+ | } | ||
+ | </style> | ||
<script> | <script> | ||
$(document).ready(function () { | $(document).ready(function () { | ||
$(window).scroll(function () { | $(window).scroll(function () { | ||
− | if ($(this).scrollTop() >= | + | if ($(this).scrollTop() >= 500) { |
var position = $("#sidelist").position(); | var position = $("#sidelist").position(); | ||
if (position == undefined) {} else { | if (position == undefined) {} else { | ||
Line 249: | Line 249: | ||
}); | }); | ||
</script> | </script> | ||
+ | <script src="https://2018.igem.org/Team:NCKU_Tainan/js/frame/T--NCKU_Tainan--jquery-1_12_4_min_js?action=raw&ctype=text/javascript"></script> | ||
+ | <script src="https://2018.igem.org/Template:NCKU_Tainan/js/bootstrap_min_js?action=raw&ctype=text/javascript"></script> | ||
</body> | </body> | ||
− | </html> | + | </html> {{NCKU_Tainan/footer}} |
− | + | ||
− | {{NCKU_Tainan/footer}} | + |
Latest revision as of 21:30, 17 October 2018