<h1 class="head">Integrated Human Practices</h1>

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                         <a class="list-group-item list-group-item-action" href="#list-item-1">Professor</a>

                         <a class="list-group-item list-group-item-action" href="#list-item-2">Meetup</a>

                         <a class="list-group-item list-group-item-action" href="#list-item-3">Enterprise Visit</a>

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                             <h10>In order to complete our project, there are lots of theoretical knowledge we have

                                 paper, the most direct and fastest way is to go to visit professors in related

                                 fields of expertise to gain their suggestion. In addition, each of professor has

                                 their specialty respectively, so accepting opinion from different professional

                                 field makes our project more comprehensive.</h10>

 

                                     <h1>Meetup with Dr. Ng I Son</h1>

                                     <div id="cardtext">Dr. Ng is the mother of NCKU-iGEM team. Since the

                                         establishment of NCKU-iGEM 2016, Dr. Ng have brought synthetic biology as

                                         well as iGEM to the flashlight of NCKU campus.</div>

                                     data-target="#NG"><a>Read More</a></div>

                                             <h5 class="modal-title" id="exampleModalLongTitle">Meetup with Dr. Ng

                                                I Son</h5>

                                             <p class="hpcontent">Dr. Ng is the mother of NCKU-iGEM team. Since the

                                                 establishment of NCKU-iGEM 2016, Dr. Ng have brought synthetic

                                                biology as well as iGEM to the flashlight of NCKU campus. Dr. Ng

                                                 every aspect. She initially gave us many critical suggestions

                                                 during the brainstorming. Except for teaching us knowledge about

                                                 advantage of protein production by E. coli, she also suggested us

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                                     <h1>Meetup with Dr. I-Hsiu</h1>

                                     <div id="cardtext">Our PI, Dr. Huang work as an assistant professor under the

                                        institute of Basic Medical Sciences of National Cheng Kung Univercity.</div>

                                     data-target="#HIS"><a>Read More</a></div>

                                             <h5 class="modal-title" id="exampleModalLongTitle">Meetup with Dr. I-Hsiu</h5>

                                             <p class="hpcontent">Our PI, Dr. Huang work as an assistant professor under

                                                 the institute of Basic Medical Sciences of National Cheng Kung

                                                 Univercity. His major field are Bacteriology, Bacteria Genetics, and

                                             <p class="hpcontent">We invited Dr. Huang to attend our first presentation.

                                                 the intention of Dr. Huang. So, our team think that Dr. Huang could

                                                 Luckily, we have succeed! </p>

                                             <p class="hpcontent">From Dr. Huang's first day as our PI, he did a lots

                                                for us. Firstly, he

                                                 provided us all the resources that we needed or could make us

                                                 convenient. Secondly, he gave us many ideas when we have met

                                                 difficulties or junction in our progress whether it is dry lab or wet

                                                 lab. We are really appreciate to what he have told and taught to us.

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                                     <h1>Meetup with Professor Bieng-Zih Hsieh

                                     <div id="cardtext">This year, our project is related to carbon sequestration.

                                         Therefore, we grab the chance to discuss our idea with Professor Bieng-Zih

                                         Hsieh.</div>

                                     data-target="#BZH"><a>Read More</a></div>

                                             <h5 class="modal-title" id="exampleModalLongTitle">Meetup with Professor

                                                 Bieng-Zih Hsieh

                                                 Professor Bieng-Zih Hsieh. Professor Bieng-Zih Hsieh is an associate

                                                 professor from the Department of Resources Engineering. One of his

                                                 research topics is about CO2 sequestration, which is a topic that

                                                 related to our project, the difference is he employs a physical method

                                             <p class="hpcontent">At first, we define our project as carbon capture.

                                                 more suitable for our project. Professor Hsieh introduced some carbon

                                                 currently and suggest us to do more research on those methods. He also

                                                 remind us to compare our system with other biological approach to

                                     <h1>Meetup with Prof. Chung

                                     <div id="cardtext">Prof. Chao Chung, Peng is an assistant professor of

                                        Degree Program on Energy. He used to work in China steel corp. so he

                                         gave us a chance to contact CSC.</div>

                                     data-target="#CCP"><a>Read More</a></div>

                                             <h5 class="modal-title" id="exampleModalLongTitle">Prof. Chao Chung, Peng

                                             <p class="hpcontent">Prof. Chao Chung, Peng is an assistant professor of

                                                 Degree Program on Energy. He used to work in China steel corp. so he

                                             <p class="hpcontent">we met up with prof. Chung to get some suggestions

                                                 which more proving

                                                 field, different from giving us experimental technical advice, he gave

                                                 us more about suggestion for action on device and idea.</p>

                                             <p class="hpcontent">He thinks that when we look for our advantages, we can

                                                 possible point that is more inefficient. The benefit of the whole

                                                 every E.coli to eat most CO2, the way we replace our cultured E.coli.

                                                 It is true that there are every existing way we can think more about

                                                 how we can improve. Also, professor tell us we must be very

                                                 understandable about our project, it includes our advantages,

                                                 most optimum environment we should provide for our E.coli. The more we

                                                 realize our project, we can find more possibility of development. After

                                             <p class="hpcontent">He gave us some advices in engineering pointview.

                                     <h1>Communication with NCHU

                                        Prof. Huang

                                     <div id="cardtext">We had an opportunity to speak with Dr. Huang Chieh-Chen from

                                         NCHU, whose

                                        work includes the reverse TCA cycle of engineered carbon capturing E. coli.</div>

                                     data-target="#NCHU"><a>Read More</a></div>

                                             <p class="hpcontent">We had an opportunity to speak with Dr. Huang

                                                 Chieh-Chen from National Chung Hsing University (NCHU) in the Asia

                                                 engineered carbon capturing E. coli. Upon our discussion, Dr. Huang

                                                 and suggested that we culture our engineered E.coli in an anaerobic

                                     <img src="https://static.igem.org/mediawiki/2018/a/a4/T--NCKU_Tainan--home_IMG_20180824_124554.jpg" class="left">

                                     <div id="cardtext"> Prof. Jo-Shu Chang complete a microalge carbon utilization

                                         system in ANAN

                                        Campus of NCKU which help the largest energy company in Taiwan.</div>

                                             <p class="hpcontent">Prof. Jo-Shu Chang was under a lot of specialty of

                                                 Biochemical Engineering, Microalgae Biotechnology, CO2 reutilization,

                                                Circular Economy; Biomass Energy, Environmental Biotechnology, Applied

                                                 Microbiology. He complete a microalge carbon utilization system in ANAN

                                                 Campus of NCKU which help the largest energy company in Taiwan (CPC,

                                                 Taiwan) and Taiwan's No.2 CO2emission company (China Steel Co.) to

                                                 develop the research platform of microalgae-based flue gas CO2

                                                 biofixation technology.Besides, Since2009, Prof. Chang served as the PI

                                                 microalgae-based CO2 capture and utilization for biofuels production

                                             <p class="hpcontent">Our project is to reuse the carbon dioxide emitted

                                                form the industrial

                                                 with a biological way. Although the pathway of carbon flow in E. coli

                                                 microalgae culture pool system including the position of CO2 pipeline,

                                                 downstream process, pool cleaning and how is the progress of an

                                                 microalgae CO2 fixation, microbial fuel cell and

                                             <p class="hpcontent">Prof. Chang let us visit how his project work in ANAN

                                                 campus(link).

                                                 Prof. Chang's team has been working with China Steel Co. and CPC,

                                                 Taiwan to develop innovative microalgae-based flue gas CO2 reduction

                                                 Prof. Chang was focus on the conversion of biomass feedstock and waste

                                                 plants were constructed in China Steel Co. and

                                             <p class="hpcontent">We visited Prof. Jo-Shu Chang discussing about our

                                                 system. We had a great time talk and he gave us three advises. One is

                                                 to define the velocity of CO2 input in which scale of CO2 we want to

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                                     <h1>Meetup with Dr. Hashimoto </h1>

                                     <div id="cardtext">Our secondary PI, Professor Hashimoto Masayuki is a

                                         project assistant professor from Institute of Molecular Medicine. </div>

                                 <h9>17</h9>

                                 <h6>APR</h6>

                                     data-target="#PHM"><a>Read More</a></div>

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                                             <h5 class="modal-title" id="exampleModalLongTitle">Meetup with Dr.

                                                 Hashimoto </h5>

                                             <p class="hpcontent">The host organism in our project is E. coli. Professor

                                                 Hashimoto is

                                                 familiar with the genetic manipulation of E. coli, therefore we

                                                 introduced our idea to him. One of his research is about the effect of

                                                 carbonic anhydrase (CA) to the growth of E. coli. Since we wanted to

                                                 introduce heterologous CA into the E. coli, he suggested us to consider

                                                 whether it will compete with the native CA of E. coli, which might

                                                 affect the function of the heterologous CA. Also, Professor Hashimoto

                                                 introduced the concept of homologous recombination to us as we had the

                                                 idea to insert our construction into E. coli chromosome. However, we

                                                 were suggested not to do the homologous recombination due to the

                                                 limited time we had. </p>

 

                                             <p class="hpcontent">After the discussion, we started to review journals

                                                 and found out the

                                                feasibility of expressing both native and non-native CA in E. coli. We

                                                 found out that there were research which expressed heterologous CA

                                                 successfully. Therefore we decided to clone the CA gene into the E

                                                 .coli. </p>

 

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                                <h1>Meetup with PhD. student, Hai-Xuan</h1>

                                <div id="cardtext">We discuss with the senior, HAI-XUAN CHENG. He is the PhD student

 

                                    <div class="modal-header">

                                         <h5 class="modal-title" id="exampleModalLongTitle">Meetup with PhD. student,

                                        <p class="hpcontent">We had an opportunity to speak with Dr. Huang Chieh-Chen

                                            University (NCHU) in the Asia Pacific Conference, whose work includes the

                                            reverse TCA cycle of

                                            engineered carbon capturing E. coli. Upon our discussion, Dr. Huang raised

                                            the issue of the low

                                            efficiency of one of our enzymes, Rubisco and suggested that we culture our

                                            carboxylase/oxygenase is an enzyme

                                            efficiency of capturing carbon dioxide due to the similarities between

                                            oxygen. </p>

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                        <h3>Meet up</h3></br>

                            and often caused resonance. What's more, we could inspire more idea and find the

                            possibility of cooperation. </h10>

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                                         <h5 class="modal-title" id="exampleModalLongTitle">Asia Pacific Conference</h5>

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                                            <span aria-hidden="true">&times;</span>

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                                        <p class="hpcontent">During July 30 to August 3, our team attended the Asia

                                            Pacific Conference is a

                                            from others and generate new ideas. These can make iGEMers have a

                                            Pacific Conference.</p>

                                         <p class="hpcontent">In these 4 days, we exhibit our project for all the

                                             iGEMers to interchange our ideas and take

                                             suggestions from others. We also found that other teams possess unique

                                             projects that may advance

                                             our new ear of technologies. We really appreciate for what we have learn in

                                            these 4 days and we

                                            hope to see them in the Giant Jamboree!</p>

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                                        <h5 class="modal-title" id="exampleModalLongTitle">Meetup with Tec-Monterrey</h5>

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                                        <p class="hpcontent">We have a video meeting with Tec-Monterrey. After

                                            our end product which can make our project more complete.</p>

                                         <p class="hpcontent">During the meeting, we came out with the idea to

                                            collaborate with each other.

                                             Their project has two

                                            part. The main part is E-coding, which use bacteria to record the data.

                                            Another part is to sense

                                            the pollutant in river of Mexico, which is the part that we could link our

                                            a pH alert system for low pH sensing, they suggest us to incorporate our ph

                                            their pollutant sensing system, making it to become more complete.</p>