Difference between revisions of "Team:NCKU Tainan/Hardware"

 
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    <link rel="stylesheet" href="https://2018.igem.org/Template:NCKU_Tainan/css/hardware?action=raw&ctype=text/css">
 
</head>
 
</head>
  
<body>
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<body data-spy="scroll" data-target=".navbar-example">
  
  <!--Page_Content-->
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    <!--Page_Content-->
  <div class="container content">
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    <div class="container content">
    <div class="navbar-example">
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         <div class="headstyle">
      <div class="row">
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             <h1 class="head">Hardware</h1>
         <div class="col-2 side">
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          <div id="sidelist" class="list-Fgroup">
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             <a class="list-group-item list-group-item-action" href="#list-item-1">Professor</a>
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            <a class="list-group-item list-group-item-action" href="#list-item-2">Event list(待訂)</a>
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            <a class="list-group-item list-group-item-action" href="#list-item-3">Cooperation Vist</a>
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            <a class="list-group-item list-group-item-action" href="#list-item-4">Event list(待訂)</a>
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            <a class="list-group-item list-group-item-action" href="#list-item-5">Meet Up</a>
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            <a class="list-group-item list-group-item-action" href="#list-item-6">Event list(待訂)</a>
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            <a class="list-group-item list-group-item-action" href="#"><i class="fa fa-arrow-up fa-1x" aria-hidden="true"></i></a>
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          </div>
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         </div>
 
         </div>
         <div class="col-10">
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         <div class="righttitle">
          <div data-spy="scroll" data-target="#sidelist" data-offset="0" class="scrollspy-example">
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            <h6 class="subtitle"> The Mini CO<sub>2</sub> Catcher</h6>
            <div class="container">
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        </div>
              <h1 class="head2">Hardware</h1>
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        <div class="navbar-example">
 +
            <div class="row">
 +
                <div class="col-2 side">
 +
                    <div id="sidelist" class="list-group">
 +
                        <a class="list-group-item list-group-item-action" href="#Accomplishment">Accomplishment</a>
 +
                        <a class="list-group-item list-group-item-action" href="#Introduction">Introduction</a>
 +
                        <a class="list-group-item list-group-item-action" href="#Device_design">Device design</a>
 +
                        <a class="list-group-item list-group-item-action" href="#Bioreactor">Bioreactor</a>
 +
                        <a class="list-group-item list-group-item-action" href="#Nutrient_tank">Nutrient tank</a>
 +
                        <a class="list-group-item list-group-item-action" href="#Electromagnetic valve">Electromagnetic valve</a>
 +
                        <a class="list-group-item list-group-item-action" href="#Materials_required">Materials required</a>
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                        <a class="list-group-item list-group-item-action" href="#"><i class="fa fa-arrow-up fa-1x"
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                                aria-hidden="true"></i></a>
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                    </div>
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                </div>
 +
                <div class="col-10">
 +
                    <div data-spy="scroll" data-target="#sidelist" data-offset="0" class="scrollspy-example">
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                        <div class="container">
  
              <div id="list-item-1">
 
                <h3>Accomplishment</h3>
 
                <p class="pcontent">
 
                  1. Built and characterized a functional prototype for carbon utilization system in industry sector.</br>
 
                  2. Created an instructional video, a manual and lists of materials.</br>
 
                  3. Implemented Bio-safety to our device.</br>
 
                  4. Integrated with modelling.</br>
 
                </p>
 
              </div>
 
  
              <div id="list-item-2">
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                            <div id="Accomplishment">
                <h3>Introduction</h3>
+
                                </br></br></br></br>
                <div id="pt">
+
                                <h3>Accomplishment</h3>
                  <p class="pcontent">Nearly 40% of CO2 emissions are attributable to industries. The goal of our
+
                                <div class="achievementborder">
                    project is to solve the CO2 problem by using engineered E.coli to fix carbon dioxide emitted from
+
                                    <ol>
                    industries and convert it into bio-product, pyruvate. To accomplish our goal, we designed a device
+
                                        <li class="bigli">Built and characterized a functional prototype for carbon utilization
                    that will upscale our project to be used on field and we aim to integrate the device into
+
                                            system in
                    industrial IGCC system. And we used the Arduino to sense the <a style="color: #28ff28;" href="#list-item-4">pH</a>(連結到hardware
+
                                            industry sector.</li>
                    pH), CO2 concentration(連結到hardware CO2) and temperature(連結到hardware temperature ) then use the
+
                                        <li class="bigli">Created an instructional video, a manual and lists of materials.</li>
                    wi-fi sensor(連結到hardware WIFI) to upload to the database. (連結到software database)Last but not least,
+
                                        <li class="bigli">Implemented Bio-safety to our device.</li>
                    we can monitor the data by our app.(連結到software app)</p>
+
                                        <li class="bigli">Integrated with <a href="https://2018.igem.org/Team:NCKU_Tainan/Model" style="color:#006030;">modeling</a>.</li>
                </div>
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                                        <li class="bigli">Installation of sensors: pH Meter, Thermometer, CO<sub>2</sub> Sensor
              </div>
+
                                            and Wi-Fi Sensor.</li>
 +
                                    </ol>
 +
                                </div>
 +
                            </div>
  
              <div id="list-item-3">
 
                <h3>Device design</h3>
 
                <div class="carousel-item active" style="background-image: url('http://placehold.it/1900x1080')"></div>
 
                <div id="pt">
 
                  <p class="pcontent">Our device consists of 3 main parts: a bioreactor, a nutrient tank and a
 
                    collection tank.The flue gas contains high concentration of CO2 and this will inhibit the growth of
 
                    E.coli. Thus, we reduce CO2 concentration level to less than 5% before entering the bioreactor by
 
                    using flowmeter, which is an instrument for measuring the flow of gases in pipelines.</p>
 
                </div>
 
              </div>
 
  
              <div id="list-item-4">
+
                            <div id="Introduction">
                <h3>Bioreactor</h3>
+
                                </br></br></br></br>
                <div id="pt">
+
                                <h3>Introduction</h3>
                  <p class="pcontent">We developed a closed bioreactor system and implemented online monitoring system
+
                                <div id="pt">
                    which can live monitoring several environmental parameters. Here is the detail of our bioreactor.</p>
+
                                    <p class="hpcontent">Nearly 30% of CO<sub>2</sub> emissions are attributable to
                </div>
+
                                        industries.
                <div class="carousel-item active" style="background-image: url('http://placehold.it/1900x1080')"></div>
+
                                        The goal of our
                <div id="pt">
+
                                        project is to solve the CO<sub>2</sub> problem by using engineered <i>E. coli</i>
                  <p class="pcontent">Gas inlet port are located on the bioreactor’s lower part while outlet port are
+
                                        to fix CO<sub>2</sub> emitted from
                    located on the bioreactor’s upper lid. As low concentration CO2 enters the bioreactor, it flows
+
                                        industries and convert it into bio-product, pyruvate. To accomplish our goal,
                    through the diffuser refiner and dissolves in the buffered medium to form acid. A pH sensor and
+
                                        we designed a device
                    temperature sensor is installed to monitor the bioreactor tank for further control implementation.
+
                                        that will upscale our project to be used on field and we aim to integrate the
                    Besides, the CO2 concentration level of exhaust gas is monitored by a CO2 gas sensor, which is
+
                                        device into
                    mounted on the upper lid. These sensor’s output is connected to an Arduino analog input and sensor
+
                                        industrial IGCC system. And we used the Arduino to sense the <a href="https://2018.igem.org/Team:NCKU_Tainan/pH_meter"
                    readings are displayed on a serial LCD which is attached on the lid of bioreactor. The data is then
+
                                            style="color:#28ff28;">pH</a>,
                    uploaded in real time to a web server via WiFi by using Arduino WiFi Shield.</p>
+
                                        <a href="https://2018.igem.org/Team:NCKU_Tainan/CO2" style="color:#28ff28;">CO<sub>2</sub>
                </div>
+
                                            concentration</a>
              </div>
+
                                        and <a href="https://2018.igem.org/Team:NCKU_Tainan/Temperature" style="color:#28ff28;">temperature</a>
 +
                                        then use the
 +
                                        <a href="https://2018.igem.org/Team:NCKU_Tainan/wi_fi" style="color:#28ff28;">Wi-Fi
 +
                                            sensor</a> to upload to the <a href="https://2018.igem.org/Team:NCKU_Tainan/Software#Database"
 +
                                            style="color:#28ff28;">database</a>. Last but not least, we can monitor the
 +
                                        condition of our device by showing data in our <a href="https://2018.igem.org/Team:NCKU_Tainan/Software#App" style="color:#28ff28;">App</a>.</p>
 +
                                </div>
 +
                                <img class="contentimg" src="https://static.igem.org/mediawiki/2018/e/ec/T--NCKU_Tainan--enterprise_hardware1.jpg">
 +
<p class="pcenter">Fig 1.Design of our device </p>
 +
                            </div>
  
  
              <div id="list-item-5">
+
                            <div id="Device_design">
                <h8>DIY Stirrer</h8></br>
+
                                </br></br></br></br>
                <div class="carousel-item active" style="background-image: url('http://placehold.it/1900x1080')"></div>
+
                                <h3>Device design</h3>
                <div id="pt">
+
                  <p class="pcontent">To prevent sedimentation of cells at the bottom of bioreactor, we build our own
+
                    3D printed slow speed magnetic stirrer which permits gentle mixing of microcarrier cell cultures.
+
                    The 3D printed magnet bed is designed specifically for two magnets and can be fitted on the DC
+
                    motor. The stirrer works by using a DC motor to spin two magnets with opposite polarity, which
+
                    could create a magnetic field in the bioreactor and cause the stir bar to spin and mix the
+
                    contents. For controlling the speed of the DC motor, we use Arduino and L298N to control the input
+
                    voltage to the motor by using PWM signal.</p>
+
                </div>
+
              </div>
+
  
              <div id="list-item-5">
+
                                <img class="contentimg" src="https://static.igem.org/mediawiki/2018/f/fa/T--NCKU_Tainan--Deviceintro.png">
                <h3>Nutrient tank(尚未完成)</h3>
+
<p class="pcenter">Fig 2.Perspective schematic view of our device </p>
                <div id="pt">
+
                  <p class="pcontent">The nutrients are pumped into the growth chamber at a rate proportional to the
+
                    growth factor of the culture, which is determined experimentally through the doubling time of the
+
                    particular bacterial strain.</p>
+
                </div>
+
              </div>
+
  
              <div id="list-item-6">
+
                                <div id="pt">
                <h3>pH Meter</h3>
+
                                    <p class="pcontent">Our device consists of 4 main parts : a bioreactor, a nutrient
                <div id="pt">
+
                                        tank, a collection
                  <p class="pcontent">
+
                                        tank and Arduino sensors. The flue gas from industrial contains high concentration of CO<sub>2</sub>  
 +
                                        which will
 +
                                        inhibit the growth
 +
                                        of <i>E.coli</i>. Thus, we will decrease CO<sub>2</sub> concentration level to less
 +
                                        than 5% at the inlet of bioreactor. With a flowmeter, we can measure the flow of gases
 +
                                        in
 +
                                        pipelines.</p>
 +
                                </div>
  
                  </p>
+
                                <img class="contentimg" src="https://static.igem.org/mediawiki/2018/f/f0/T--NCKU_Tainan--device.jpg">
                </div>
+
<p class="pcenter">Fig 3.Circuit diagram</p>
              </div>
+
                                <div id="pt">
 +
                                    <p class="pcontent">For Arduino, we use thermometer (DS18B20)、pH meter and
 +
                                        CO<sub>2</sub> sensor (MG811) to
 +
                                        monitor our device. Besides, the LCD will print datum while the Wi-Fi
 +
                                        sensor (ESP8266 Nodemcu) will
 +
                                        upload our records to database. You can see more information about
 +
                                        arduino code in <a href="https://2018.igem.org/Team:NCKU_Tainan/Software" style="color:#28ff28;">software</a>.</p>
 +
                                </div>
 +
                            </div>
 +
 
 +
 
 +
                            <div id="Bioreactor">
 +
                                </br></br></br></br>
 +
                                <h3>Bioreactor</h3>
 +
                                <div id="pt">
 +
                                    <p class="pcontent">We developed a closed system on in our bioreactor design and implemented online
 +
                                      real time monitoring system
 +
                                        which can determine the progress condition of bioreactor.</p>
 +
                                </div>
  
              <div id="list-item-7">
+
                                <div id="pt">
                <h3>Materials required</h3>
+
                                    <p class="pcontent">The gas inlet port is located on the bioreactor’s lower part while
                <div id="pt">
+
                                        outlet port is
                  <p class="pcontent">
+
                                        located on the bioreactor’s upper lid. As low concentration CO<sub>2</sub>
 +
                                        enters the
 +
                                        bioreactor, it flows
 +
                                        through the diffuser refiner and dissolves in the buffered medium to form acid.
 +
                                        A pH sensor and
 +
                                        a thermometer is installed to monitor the bioreactor tank for further
 +
                                        control implementation.
 +
                                        Besides, the CO<sub>2</sub> concentration level of exhaust gas is monitored by
 +
                                        a CO<sub>2</sub> 
 +
                                        sensor, which is
 +
                                        mounted on the upper lid. These sensor’s output is connected to an Arduino
 +
                                        analog input and sensor
 +
                                        readings are displayed on a serial LCD which is attached on the lid of
 +
                                        bioreactor. The data is then
 +
                                        uploaded in real time to a web server via WiFi by using Arduino WiFi Shield.</br></p>
 +
                                </div>
 +
                                </br>
 +
                                <h8>Stirrer</h8></br>
  
                  </p>
+
                                <img class="contentimg" src="https://static.igem.org/mediawiki/2018/6/69/T--NCKU_Tainan--Capture.PNG">
 +
<p class="pcenter">Fig 4.Perspective schematic view of magnetic stir</p>
 +
                                <div id="pt">
 +
                                    <p class="pcontent">To prevent sedimentation of cells at the bottom of bioreactor,
 +
                                        we build our own
 +
                                        slow speed magnetic stirrer of 3D printed materials which permits gentle mixing of
 +
                                        microcarrier cell cultures.
 +
                                        The 3D printed magnet bed is designed specifically for two magnets and can be
 +
                                        fitted on the DC
 +
                                        motor. The stirrer works by using a DC motor to spin two magnets with opposite
 +
                                        polarity, which
 +
                                        could create a magnetic field in the bioreactor and cause the stir bar to spin
 +
                                        and mix the
 +
                                        contents. For controlling the speed of the DC motor, we use Arduino and L298N
 +
                                        to control the input
 +
                                        voltage to the motor by using PWM signal.</p>
 +
                                <img class="contentimg" src="https://static.igem.org/mediawiki/2018/8/8b/T--NCKU_Tainan--magntic_stir_real.PNG">
 +
<p class="pcenter">Fig 5.Design of our magnetic stir</p>
 +
                                </div>
 +
                            </div>
 +
 
 +
   
 +
 
 +
</br></br>
 +
                            <div id="Nutrient_tank">
 +
                                </br></br></br></br>
 +
                                <h3>Nutrient tank</h3>
 +
                                <div id="pt">
 +
                                    <p class="pcontent">Besides, we also implemented fed-batch culture system in our
 +
                                        design. Nutrients are fed to the bioreactor during cultivation to prevent
 +
                                        nutrient depletion. The nutrients are pumped into the growth chamber at a rate
 +
                                        proportional to the growth factor of the culture, which is determined
 +
                                        experimentally through the doubling time of the particular bacterial strain.</p>
 +
                                </div>
 +
                            </div>
 +
          </br></br>
 +
<div id="Electromagnetic valve">
 +
                                </br></br></br></br>                   
 +
                                <h3>Electromagnetic valve</h3>
 +
                                <div id="pt">
 +
                                    <p class="pcontent">In order to simulate the situation of the industry, two electromagnetic valves are installed on the input and output of the collection tank and the medium tank, they can be controlled by the App.</p>
 +
                                </div>
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                            </div>
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 +
                            <div id="Materials_required">
 +
                                </br></br></br></br>
 +
                                <h3>Materials required</h3>
 +
                                <div id="pt">
 +
                                    <ul>
 +
                                        <li>Acrylic Sheet</li>
 +
                                        <li>Arduino UNO</li>
 +
                                        <li>Power Supply</li>
 +
                                        <li>Batteries</li>
 +
                                        <li>Rotameter</li>
 +
                                        <li>pH meter</li>
 +
                                        <li>Thermometer (DS18B20)</li>
 +
                                        <li>CO<sub>2</sub> sensor (MG811)</li>
 +
                                        <li>Wi-Fi sensor (ESP8266 NodeMcu)</li>
 +
                                        <li>Geared DC Motor</li>
 +
                                        <li>Tubes</li>
 +
                                        <li>Magnets</li>
 +
                                        <li>3D Printed Structure</li>
 +
                                        <li>Nuts and Screws</li>
 +
                                        <li>Wires</li>
 +
                                        <li>Pumps</li>
 +
                                    </ul>
 +
                                    </br></br></br></br>
 +
                                </div>
 +
                            </div>
 +
 
 +
                        </div>
 +
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Latest revision as of 21:42, 17 October 2018

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