Difference between revisions of "Team:NKU CHINA/Attributions"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Attributions</h1>
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<p>This page is your opportunity to explain what parts of your project you did and what was done by technicians, advisers, etc. This requirement is not about literature references - these can and should be displayed throughout your wiki.
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<h3> Bronze Medal Criterion #3</h3>
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<p> All of the work done in your project must be attributed correctly on this page. You must clearly state the work that was done by the students on your team and note any work that was done by people outside of your team, including the host labs, advisors, instructors, and individuals not on the team roster.
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Please see the <a href="https://2018.igem.org/Judging/Medals">Medals requirements page</a> for more details.</p>
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<h3> What should this page contain?</h3>
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<ul>
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<li>Clearly state what the team accomplished</li>
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<li>General Support</li>
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<li>Project support and advice</li>
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<li>Fundraising help and advice</li>
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<li>Lab support</li>
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<li>Difficult technique support</li>
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<li>Project advisor support</li>
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<li>Wiki support</li>
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<li>Presentation coaching</li>
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<li>Human Practices support</li>
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<li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</li>
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</ul>
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<p>Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:</p>
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<ul>
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<li>Does your institution teach an iGEM or synthetic biology course?</li>
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<li>When did you start this course?</li>
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<li>Are the syllabus and course materials freely available online?</li>
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<li>When did you start your brainstorming?</li>
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<li>When did you start in the lab?</li>
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<li>When did you start working on  your project?</li>
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<h3>Inspiration</h3>
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<p>Take a look at what other teams have done:</p>
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<ul>
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<li><a href="https://2011.igem.org/Team:Imperial_College_London/Team">2011 Imperial College London</a> (scroll to the bottom)</li>
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<li><a href="https://2014.igem.org/Team:Exeter/Attributions">2014 Exeter </a></li>
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<li><a href="https://2014.igem.org/Team:Melbourne/Attributions">2014 Melbourne </a></li>
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<li><a href="https://2014.igem.org/Team:Valencia_Biocampus/Attributions">2014 Valencia Biocampus</a></li>
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</ul>
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<h3> Why is this page needed? </h3>
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<p>The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.</p>
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<p>
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For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.</p>
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<h3> Can we base our project on a previous one? </h3>
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<p>Yes! You can have a project based on a previous team, or based on someone else's idea, <b>as long as you state this fact very clearly and give credit for the original project.</b> </p>
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        <div class="fullscreenvideo" >
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        <video id="attrbg" autoplay="autoplay" muted="muted" loop="loop">
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          <source src="https://static.igem.org/mediawiki/2018/f/fb/T--NKU_CHINA--BGofother.mp4" type="video/mp4">
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        </video>
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        </div>
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  </div>
  
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    <main>
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        <h1 class="text-center" style="font-size: 60px;font-weight: normal;color: white; font-family: myTitle;">Attributions in <span data-toogle="tooltip" data-placement="bottom" title="Population Quality Control">PopQC</span> System Construction</h3>
 +
        <div class="container-fluid">
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            <div class="col-xs-3 col-xs-push-1">
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                <img src="https://static.igem.org/mediawiki/2018/c/ca/T--NKU_CHINA--DuiHui.png" class="img-responsive attribution_logo" style="margin-top: 50px;">
 +
            </div>
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            <div class="col-xs-6 col-xs-push-2" style="margin-top: 40px;">
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            <h3 style="color: #FFD600;font-size: 28px;">Zeyu Zhou, Zhihao Yan, Lin Luan:</h3>
 +
            <ul style="list-style:disc;line-height: 30px;">
 +
              <li style="color: white;font-size: 23px;text-align: left;">Construction of pHT01-P<sub><i>gltAB/gltX/ldh/ugt/arg/accd</i></sub>-<i>lacI</i>-P<sub><i>grac</i></sub>-<i>tetA</i>.</li> 
 +
              <li style="color:white;font-size:23px;text-align: left;">Fermentation of pHT01-P<sub><i>gltAB</i></sub>-<i>lacI</i>-P<sub><i>grac</i></sub>-<i>tetA</i> in <i>Bacillus amyloliquefaciens</i> LL3 to measure its &#947;-PGA yield.</li>
 +
              <li style="color: white;font-size: 23px;">Fermentation of pHT01-P<sub><i>gltAB</i></sub>/P<sub>43</sub>-GFP in <i>Bacillus</i> sp. for measuring their OD<sub>600</sub>, the fluorescence of GFP and the expression level of GltC.</li>
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            </ul>
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            </div>
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        </div>
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        <div class="container-fluid">
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          <div class="col-xs-10 col-xs-push-1">
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                <h3 style="color: #FFD600;font-size: 28px;">Ziyu Wang, Danqing Tong, Senhao Kou, Fanyang He:</h3>
 +
                <ul style="list-style:disc; line-height: 30px;">
 +
                  <li style="color: white;font-size: 23px;">Genome editing in <i>Bacillus amyloliquefaciens</i> LL3 and <i>Bacillus subtilis</i> 168 on glutamate related genes’ promoters for higher glutamate concentration, for example, <i>icd</i> and <i>gltAB</i>.</li>
 +
                  <li style="color: white;font-size: 23px;">Construction of pHT01-P<sub><i>gltAB</i></sub>/P<sub>43</sub>-GFP. Fermentation of pHT01-P<sub><i>gltAB</i></sub>/P<sub>43</sub>-GFP in <i>Bacillus</i> sp. to verify the relationship of glutamate concentration and the effect of P<sub><i>gltAB</i></sub>.</li>
 +
                  <li style="color: white;font-size: 23px;">Construction of pHT01-<i>mCherry</i>-<i>lacI</i>-P<sub><i>gltAB</i></sub>-P<sub><i>grac</i></sub>-<i>tetA</i>-GFP(int). Fermentation under different glutamate concentration for measuring OD<sub>600</sub> and FI of <i>mCherry</i> and GFP and mRNA in <i>Bacillus amyloliquefaciens</i> LL3 transformed with pHT01-<i>mCherry</i>-<i>lacI</i>-P<sub><i>gltAB</i></sub>-P<sub><i>grac</i></sub>-<i>tetA</i>-GFP(int).</li>
 +
                </ul>
 +
          </div>
 +
          <div class="col-xs-5 col-xs-push-1">
 +
            <h3 style="color: #FFD600;font-size: 28px;">Lin Luan, Senhao Kou:</h3>
 +
            <p style="color: white;font-size: 23px;">InterLab Study</p>         
 +
            <h3 style="color: #FFD600;font-size: 28px;margin-top: 0;">Min Zheng:</h3>
 +
            <p style="color: white;font-size: 23px;">Wiki Construction</p>
 +
            <h3 style="color: #FFD600;font-size: 28px;margin-top: 0;">Guanyue Xiong:</h3>
 +
            <p style="color: white;font-size: 23px;">Mathematical Modeling; Human Practice</p>
 +
          </div>
 +
          <div class="col-xs-5 col-xs-push-1">
 +
            <h3 style="color: #FFD600;font-size: 28px;">Ziyu Wang, Danqing Tong:</h3>
 +
            <p style="color: white;font-size: 23px;text-align: justify;"><strong>Parts improvements</strong> (a. Enhance the promoter P<sub><i>liaG</i></sub>(BBa_K823000) of <i>Bacillus subtillis</i> in the iGEM part by mimic the structure of P<sub>43</sub> and test with pHT01-GFP promoter testing system. b. Construction of pHT01-P<sub>43</sub>-<i>tetA</i> (codon optimized) and pHT01-P<sub>43</sub>-<i>tetA</i>(kit) and then testing the function of the codon optimized one.) and <strong>construction</strong>.</p>
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          </div>
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          </div>
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          <div class="container-fluid">
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          <div class="col-xs-5 col-xs-push-1">
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            <h3 style="color: #FFD600;font-size: 28px;">Yu Zhao, Qiuhan Lang: <span style="color:white;">Human Practice</span></h3>
 +
            <p style="color: white;font-size: 23px;text-align: justify;">Investigation and propaganda on synthetic biology and iGEM to high school students and the society. Social practice in western China. Consult and communicate with Tianjin Institute of Industrial Biotechnology, Tianjin International Joint Academy Biomedicine, and a famous biotech company named Novozymes. Conferences participating like CCiC and the iGEM league conference in Beijing-Tianjin region.</p>
 +
          </div>
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          <div class="col-xs-5 col-xs-push-1">
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          <h3 style="color: #FFD600;font-size: 28px;">Zhaolin Ma, Zixin Zhao, Bo Xu: <span style="color:white;">Art Design</span></h3>
 +
          <p style="color: white;font-size: 23px;text-align: justify;">Design our team logo, characters, story, refrigerator magnets and uniforms. Draw up the experiment flow chart. Make a folding of our project for Human practice group to conduct propaganda. Poster and PPT making. Help wiki group to establish website. Team video making, pictures collecting and voice recording. Design and upload team banner.</p>
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          </div>
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          </div>
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          <div class="container">
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            <div class="row">
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              <div class="col-xs-12">
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                <h2 style="color: white;font-size: 50px;text-align: center;font-family: myTitle3;">Supervision</h2>
 +
                <ul class="list-unstyled">
 +
                  <li style="color: white;"><h3 style="font-size: 25px;">Cunjiang Song:</h3><p style="font-size: 23px;">Prof. Cunjiang Song contributed greatly to the initial idea of this project and gave us lots of inspirations. Unfortunately, he’s gone forever. We always miss our beloved teacher.</p></li>
 +
                  <li style="color: white;"><h3 style="font-size: 25px;">Zhihui Cheng:</h3><p style="font-size: 23px;">Prof. Zhihui Cheng is an extremely responsible primary PI. He has been pushing our project to progress normally from the very beginning.</p></li>
 +
                  <li style="color: white;"><h3 style="font-size: 25px;">Chao Yang:</h3><p style="font-size: 23px;">Prof. Chao Yang provided us with experimental equipment and materials. He also helped us find solutions when our experiments went wrong.</p></li>
 +
                  <li style="color: white;"><h3 style="font-size: 25px;">Weixia Gao:</h3><p style="font-size: 23px;">As an instructor of our team, Weixia Gao not only gave us clear and essential instructions, but also offered us the most direct assistance. She also helped us with the construction of <i>B. amyloliquefaciens</i> LL3 Asp auxotroph.</p></li>
 +
                </ul>
 +
              </div>
 +
            </div>
 +
          </div>
 +
          <div class="container">
 +
            <div class="row">
 +
              <div class="col-xs-12">
 +
                <h2 style="color: white;font-size: 50px;text-align: center;font-family: myTitle3;">Acknowledgements</h2>
 +
                <p style="font-size: 23px;color: white;text-align: justify;">We would like to thank the College of Life Sciences and Boling College for care and support. We would like to thank the Key Laboratory of Molecular Microbiology and Technology, Ministry of Education. We would like to thank the Prof. Ting Ma for offering us spectrophotometer and high pressure liquid chromatography and also thank him for providing us with opportunities to communicate with the Novozymes company. We would like to thank Prof. Huifeng Jiang and Prof. Changhao Bi for giving us useful advice on experimental design and operation.</p>
 +
                <p style="font-size: 23px;color: white;text-align: justify;">We would like to thank the Tianjin Institute of Industrial Biotechnology, Tianjin International Joint Academy Biomedicine and Novozymes for providing communicating chance to us and giving us a perfect platform to present our project.</p>
 +
                <p style="font-size: 23px;color: white;text-align: justify;">Finally, we want to thank teams listed below for helping us improve our project on data process and analysis, modelling, human practice, etc.</p>
 +
                <ul style="font-size: 23px;color: white;list-style:disc;">
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                  <li>iGEM Team NPU-China;</li>
 +
                  <li>iGEM Team Peking;</li>
 +
                  <li>iGEM Team Tianjin;</li>
 +
                  <li>iGEM Team TUST_China;</li>
 +
                  <li>iGEM Team UCAS-China.</li>
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                </ul>
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              </div>
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            </div>
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          </div>
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    </main>
  
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<footer>
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<div class="container-fluid myFooter">
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<div class="row">
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<div class="col-xs-8 col-xs-push-1">
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<img src="https://static.igem.org/mediawiki/2018/0/0b/T--NKU_CHINA--duihui_final.png" style="height: 250px; width: auto;">
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</div>
 +
<div class="col-xs-4 footer-w3ls">
 +
<h3 style="color: white; font-size: 30px;">Contacts</h3>
 +
<ul class="social-icons1" style="margin:0 auto;padding-top:0;">
 +
<li><a href="https://www.facebook.com/iGEMNKU2018/"><i class="fa fa-facebook" aria-hidden="true"></i></a></li>
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<li><a href="https://twitter.com/iGEMNKU2018"><i class="fa fa-twitter" aria-hidden="true"></i></a></li>
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<li><a href="mailto:nkuigem2018@163.com"><i class="fa fa fa-envelope-o" aria-hidden="true"></i></a></li>
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    </ul>
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    <h3 style="color: white; font-size: 30px;">Address</h3>
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                <address style="color: white; font-size: 17px;">
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                    Nankai University</br>
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                    No.94 Weijin Road, Nankai District</br>
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                    Tianjin, P.R.China 300071</br>
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                </address>
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            </div>
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            <div class="col-xs-12">
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            <p class="Copyright" style="text-align: center; color: white;">Copyright © Team NKU_CHINA 2018</p>
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Latest revision as of 21:50, 17 October 2018

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Attributions in PopQC System Construction

Zeyu Zhou, Zhihao Yan, Lin Luan:

  • Construction of pHT01-PgltAB/gltX/ldh/ugt/arg/accd-lacI-Pgrac-tetA.
  • Fermentation of pHT01-PgltAB-lacI-Pgrac-tetA in Bacillus amyloliquefaciens LL3 to measure its γ-PGA yield.
  • Fermentation of pHT01-PgltAB/P43-GFP in Bacillus sp. for measuring their OD600, the fluorescence of GFP and the expression level of GltC.

Ziyu Wang, Danqing Tong, Senhao Kou, Fanyang He:

  • Genome editing in Bacillus amyloliquefaciens LL3 and Bacillus subtilis 168 on glutamate related genes’ promoters for higher glutamate concentration, for example, icd and gltAB.
  • Construction of pHT01-PgltAB/P43-GFP. Fermentation of pHT01-PgltAB/P43-GFP in Bacillus sp. to verify the relationship of glutamate concentration and the effect of PgltAB.
  • Construction of pHT01-mCherry-lacI-PgltAB-Pgrac-tetA-GFP(int). Fermentation under different glutamate concentration for measuring OD600 and FI of mCherry and GFP and mRNA in Bacillus amyloliquefaciens LL3 transformed with pHT01-mCherry-lacI-PgltAB-Pgrac-tetA-GFP(int).

Lin Luan, Senhao Kou:

InterLab Study

Min Zheng:

Wiki Construction

Guanyue Xiong:

Mathematical Modeling; Human Practice

Ziyu Wang, Danqing Tong:

Parts improvements (a. Enhance the promoter PliaG(BBa_K823000) of Bacillus subtillis in the iGEM part by mimic the structure of P43 and test with pHT01-GFP promoter testing system. b. Construction of pHT01-P43-tetA (codon optimized) and pHT01-P43-tetA(kit) and then testing the function of the codon optimized one.) and construction.

Yu Zhao, Qiuhan Lang: Human Practice

Investigation and propaganda on synthetic biology and iGEM to high school students and the society. Social practice in western China. Consult and communicate with Tianjin Institute of Industrial Biotechnology, Tianjin International Joint Academy Biomedicine, and a famous biotech company named Novozymes. Conferences participating like CCiC and the iGEM league conference in Beijing-Tianjin region.

Zhaolin Ma, Zixin Zhao, Bo Xu: Art Design

Design our team logo, characters, story, refrigerator magnets and uniforms. Draw up the experiment flow chart. Make a folding of our project for Human practice group to conduct propaganda. Poster and PPT making. Help wiki group to establish website. Team video making, pictures collecting and voice recording. Design and upload team banner.

Supervision

  • Cunjiang Song:

    Prof. Cunjiang Song contributed greatly to the initial idea of this project and gave us lots of inspirations. Unfortunately, he’s gone forever. We always miss our beloved teacher.

  • Zhihui Cheng:

    Prof. Zhihui Cheng is an extremely responsible primary PI. He has been pushing our project to progress normally from the very beginning.

  • Chao Yang:

    Prof. Chao Yang provided us with experimental equipment and materials. He also helped us find solutions when our experiments went wrong.

  • Weixia Gao:

    As an instructor of our team, Weixia Gao not only gave us clear and essential instructions, but also offered us the most direct assistance. She also helped us with the construction of B. amyloliquefaciens LL3 Asp auxotroph.

Acknowledgements

We would like to thank the College of Life Sciences and Boling College for care and support. We would like to thank the Key Laboratory of Molecular Microbiology and Technology, Ministry of Education. We would like to thank the Prof. Ting Ma for offering us spectrophotometer and high pressure liquid chromatography and also thank him for providing us with opportunities to communicate with the Novozymes company. We would like to thank Prof. Huifeng Jiang and Prof. Changhao Bi for giving us useful advice on experimental design and operation.

We would like to thank the Tianjin Institute of Industrial Biotechnology, Tianjin International Joint Academy Biomedicine and Novozymes for providing communicating chance to us and giving us a perfect platform to present our project.

Finally, we want to thank teams listed below for helping us improve our project on data process and analysis, modelling, human practice, etc.

  • iGEM Team NPU-China;
  • iGEM Team Peking;
  • iGEM Team Tianjin;
  • iGEM Team TUST_China;
  • iGEM Team UCAS-China.

Contacts

Address

Nankai University
No.94 Weijin Road, Nankai District
Tianjin, P.R.China 300071

Copyright © Team NKU_CHINA 2018

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