BASIC PARTS

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Parts Deposited On the Registry

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Cerberus : A Molecular Binding Plateform (mSA2-CBM3a-AzF)

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This part consists on a CBM3a fused with mSA2, an engineered version of streptavidin and rhizavidin, on his N-terminus endogenous linker and containing a UAG codon on his C-terminus linker (amber stop codon) for the incorporation of the unnatural amino acid. Note that this part encodes the Cerberus platform if AzF is provided and the appropriate tRNA synthetase is expressed. Otherwise, it encodes for the Orthos construction.
For more details about the design, refer to the corresponding registry page.

Sirius: Cellulose-Binding Modules of Type 3a (CBM3a) Fused to mRFP1

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This part results in the fusion of the fluorescent protein mRFP1 to the cellulose binding domain CBM3a. The aim of this construction is to prove the binding of CBM3a to cellulose and to quantify it by fluorescence. Furthermore, the mRFP1 is visible to the naked eye and can therefore be used as a visual proof of concept.
For more details about the design, refer to the corresponding registry page.

mTagBFP Avi-tag

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mTag Blue Fluorescent Protein fused with an Avi-tag for in vivo biotinylation by BirA.
For more details about the design, refer to the corresponding registry page.