Difference between revisions of "Team:Toulouse-INSA-UPS/Basic Part"

(Prototype team page)
 
 
(11 intermediate revisions by 4 users not shown)
Line 1: Line 1:
−
{{Toulouse-INSA-UPS}}
+
<!--THIS COMES BEFORE THE CONTENT-->
−
<html>
+
{{Template:Toulouse-INSA-UPS/SCROLLSPY}} <html>
 +
  <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:Toulouse-INSA-UPS/BS-CSS&action=raw&ctype=text/css"/>
 +
  <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:Toulouse-INSA-UPS/PERSO-CSS&action=raw&ctype=text/css"/>
  
 +
    <!--Banner-->
 +
<div class="container-fluid parallax" style="background-image: url('https://static.igem.org/mediawiki/2018/b/ba/T--Toulouse-INSA-UPS--Parts--Brice--Banner.jpg');  background-size: cover; background-position-y: center;" id="BANNER"></div>
  
−
<div class="column full_size judges-will-not-evaluate">
+
</html> {{Template:Toulouse-INSA-UPS/MENU}} <html>
−
<h3>★  ALERT! </h3>
+
</div>
−
<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
+
</html> {{Template:Toulouse-INSA-UPS/CONTENT-BEGINNING}} <html>
−
<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
+
<!--CONTENT COMES HERE-->
−
</div>
+
  
 +
<div class="column full_size">
 +
<h1 class="heavy">BASIC PARTS</h1><hr/>
  
−
<div class="clear"></div>
+
    <h2 class="heavy">Parts Deposited On the Registry</h2><hr/>
 +
        <h3>Cerberus : A Molecular Binding Plateform (mSA2-CBM3a-AzF)</h3><hr/>
 +
        <div style="text-align:center">
 +
<figure class="figure"  style="text-align:center;">
 +
                <img style="width : 70%; heigth = auto;" src="https://static.igem.org/mediawiki/2018/4/4f/T--Toulouse-INSA-UPS--Parts--Youn--Cerberus.PNG" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure.">
 +
                <figcaption class="figure-caption"><strong>Figure 1:</strong> <i>Part BBa_K2668010</i></figcaption>
 +
            </figure>
 +
</div>
 +
        <p>
 +
                This part consists on a CBM3a fused with mSA2, an engineered version of streptavidin and rhizavidin, on his <i>N-</i>terminus endogenous linker and containing a UAG codon on his <i>C-</i>terminus linker (amber stop codon) for the incorporation of the unnatural amino acid. Note that this part encodes the Cerberus platform if AzF is provided and the appropriate tRNA synthetase is expressed. Otherwise, it encodes for the Orthos construction.
 +
                <br/>For more details about the design, refer to the corresponding registry <a href="http://parts.igem.org/Part:BBa_K2668010" target="_blank">page</a>.
 +
        </p>
 +
        <br/>
 +
<h3>Sirius: Cellulose-Binding Modules of Type 3a (CBM3a) Fused to mRFP1</h3>
 +
<hr/>
 +
<div style="text-align:center">
 +
<figure class="figure"  style="text-align:center;">
 +
<img style="width : 70%; heigth = auto;" src="https://static.igem.org/mediawiki/2018/0/04/T--Toulouse-INSA-UPS--Parts--Youn--Sirius.PNG" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure.">
 +
<figcaption class="figure-caption"><strong>Figure 2:</strong> <i>Part BBa_K2668020</i></figcaption>
 +
</figure>
 +
</div>
 +
<p>
 +
This part results in the fusion of the fluorescent protein mRFP1 to the cellulose binding domain CBM3a. The aim of this construction is to prove the binding of CBM3a to cellulose and to quantify it by fluorescence. Furthermore, the mRFP1 is visible to the naked eye and can therefore be used as a visual proof of concept.
 +
<br/>For more details about the design, refer to the corresponding registry <a href="http://parts.igem.org/Part:BBa_K2668020" target="_blank">page</a>.
 +
</p>
 +
<br/>
  
 +
<h3>mTagBFP Avi-tag</h3><hr/>
 +
<div style="text-align:center">
 +
<figure class="figure"  style="text-align:center;">
 +
<img style="width : 70%; heigth = auto;" src="https://static.igem.org/mediawiki/2018/d/dc/T--Toulouse-INSA-UPS--Parts--Youn--BFP.PNG" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure.">
 +
<figcaption class="figure-caption"><strong>Figure 3:</strong> <i>Part BBa_K2668070</i></figcaption>
 +
</figure>
 +
</div>
 +
<p>
 +
mTag Blue Fluorescent Protein fused with an Avi-tag for <i>in vivo</i> biotinylation by BirA.
 +
<br/>For more details about the design, refer to the corresponding registry <a href="http://parts.igem.org/Part:BBa_K2668070" target="_blank">page</a>.
 +
</p>
  
  
−
<div class="column full_size">
 
−
<h1>Basic Parts</h1>
 
−
<p>
 
−
A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
 
−
</p>
 
  
−
<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
 
 
</div>
 
</div>
  
−
 
−
<div class="column full_size">
 
−
<div class="highlight decoration_background">
 
−
<h3>Note</h3>
 
−
<p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!</p>
 
−
</div>
 
−
</div>
 
−
 
−
 
−
<div class="column full_size">
 
−
<h3>Best Basic Part Special Prize</h3>
 
−
 
−
<p> To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2018.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
 
−
 
−
<br><br>
 
−
<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
 
−
</div>
 
  
  
Line 45: Line 63:
  
  
 +
<!--CONTENT ENDS HERE-->
 +
</div></html> {{Template:Toulouse-INSA-UPS/CONTENT-END}} <html>
 +
<div id="NAV_ICON_BAR" class="nav-left-col sticky-bottom d-none d-xl-block">
 +
<ul class="nav justify-content-center">
 +
  <li class="nav-item">
 +
<!--Anchor to TOP-->
 +
  <a class="nav-link ico" href="#TOP">
 +
<img class="ico" src="https://static.igem.org/mediawiki/2018/1/12/T--Toulouse-INSA-UPS--All--Yohann--Up_Arrow.png" alt="Up Arrow"/>
 +
</a>
 +
  </li>
 +
</ul>
 +
</div>
 +
</html> {{Template:Toulouse-INSA-UPS/FOOTER}} <html>
 +
<script type="text/javascript" src="https://2018.igem.org/wiki/index.php?title=Template:Toulouse-INSA-UPS/JQ-JS&action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2018.igem.org/wiki/index.php?title=Template:Toulouse-INSA-UPS/POPPER-JS&action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2018.igem.org/wiki/index.php?title=Template:Toulouse-INSA-UPS/BS-JS&action=raw&ctype=text/javascript"></script>
 
</html>
 
</html>
 +
<!--OUR WORK ENDS HERE-->

Latest revision as of 22:23, 17 October 2018

BASIC PARTS

Parts Deposited On the Registry

Cerberus : A Molecular Binding Plateform (mSA2-CBM3a-AzF)

A generic square placeholder image with rounded corners in a figure.
Figure 1: Part BBa_K2668010

This part consists on a CBM3a fused with mSA2, an engineered version of streptavidin and rhizavidin, on his N-terminus endogenous linker and containing a UAG codon on his C-terminus linker (amber stop codon) for the incorporation of the unnatural amino acid. Note that this part encodes the Cerberus platform if AzF is provided and the appropriate tRNA synthetase is expressed. Otherwise, it encodes for the Orthos construction.
For more details about the design, refer to the corresponding registry page.


Sirius: Cellulose-Binding Modules of Type 3a (CBM3a) Fused to mRFP1

A generic square placeholder image with rounded corners in a figure.
Figure 2: Part BBa_K2668020

This part results in the fusion of the fluorescent protein mRFP1 to the cellulose binding domain CBM3a. The aim of this construction is to prove the binding of CBM3a to cellulose and to quantify it by fluorescence. Furthermore, the mRFP1 is visible to the naked eye and can therefore be used as a visual proof of concept.
For more details about the design, refer to the corresponding registry page.


mTagBFP Avi-tag

A generic square placeholder image with rounded corners in a figure.
Figure 3: Part BBa_K2668070

mTag Blue Fluorescent Protein fused with an Avi-tag for in vivo biotinylation by BirA.
For more details about the design, refer to the corresponding registry page.

No dogs were harmed over the course of this iGEM project.

INSA Toulouse UPS LISBP TWB Defi Adisseo Foundation ESF Catalyses


The content provided on this website is the fruit of the work of the Toulouse INSA-UPS iGEM Team. As a deliverable for the iGEM Competition, it falls under the Creative Commons Attribution 4.0. Thus, all content on this wiki is available under the Creative Commons Attribution 4.0 license (or any later version). For futher information, please consult the official website of Creative Commons.

This website was designed with Bootstrap (4.1.3). Bootstrap is a front-end library of component for html, css and javascript. It relies on both Popper and jQuery. For further information, please consult the official website of Bootstrap.