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<img class="smallimg" src="https://static.igem.org/mediawiki/2018/6/65/T--NCKU_Tainan--of_course.png" alt="of course"> | <img class="smallimg" src="https://static.igem.org/mediawiki/2018/6/65/T--NCKU_Tainan--of_course.png" alt="of course"> | ||
</div> | </div> | ||
− | <p class="pcontent">In order to reduce the concentration of carbon dioxide which is surrounding in environment, | + | <p class="pcontent">In order to reduce the concentration of carbon dioxide |
− | our team has developed a device, containing our engineered <i>E. coli</i> to fix the | + | (CO<sub>2</sub>) which is surrounding in environment, |
+ | our team has developed a device, containing our engineered <i>E. coli</i> to fix the | ||
+ | CO<sub>2</sub> | ||
when it undergoes its native metabolic pathway. | when it undergoes its native metabolic pathway. | ||
− | Those three data, temperature, concentration of | + | Those three data, temperature, concentration of CO<sub>2</sub>, |
and pH value will be shown on the screen as we have developed an App for our user. | and pH value will be shown on the screen as we have developed an App for our user. | ||
− | All of our ideas are to provide an another choice for public without any replacement as we are really longing for the low carbon society. | + | All of our ideas are to provide an another choice for public without any replacement |
− | Therefore, we use microorganism to , | + | as we are really longing for the low carbon society. |
+ | Therefore, we use microorganism to, "of CO<sub>2</sub>urse", reduce the carbon dioxide | ||
+ | in the atmosphere. | ||
</p> | </p> | ||
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in the current society since the awareness of industrial evolution in the 18<sup>th</sup> century. | in the current society since the awareness of industrial evolution in the 18<sup>th</sup> century. | ||
</p> | </p> | ||
− | <p class="pcontent">Greenhouse effect brings a lot of side | + | <p class="pcontent">Greenhouse effect brings a lot of side effect to us. |
− | For example, ice | + | For example, ice will melt, and it causes the rise of sea level while the land for terrestrial |
− | + | to live is getting lesser and lesser. Besides, the global temperature will increase resulting the rise of sea temperature, | |
− | and that rise of temperature will make the chemical reaction in the sea to be faster than usual | + | and that rise of temperature will make the chemical reaction in the sea to be faster than usual depleting the coral reef which unbalance the ecosystem in the sea. |
− | + | ||
Furthermore, greenhouse effect will also be resulting the depletion of the ozone layer. | Furthermore, greenhouse effect will also be resulting the depletion of the ozone layer. | ||
It means that living things will exposed under ultraviolet light and all the living things will live | It means that living things will exposed under ultraviolet light and all the living things will live | ||
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we will face the risk of extinction as the situation is getting worse. | we will face the risk of extinction as the situation is getting worse. | ||
As one of the members in global village, our team, | As one of the members in global village, our team, | ||
− | NCKU_Tainan | + | NCKU_Tainan think that we should enroll ourselves in movement that can help reduce the emission of |
− | greenhouse gaseous (GHGs). This year, our team designed a new way to catch one of the GHGs, | + | greenhouse gaseous (GHGs). This year, our team have designed a new way to catch one of the GHGs, |
− | + | CO<sub>2</sub>, by using an advance and modern technique nowadays, Synthetic Biology. | |
Without any replacement with other ways but to offer another way to public, | Without any replacement with other ways but to offer another way to public, | ||
− | the public could | + | the public could use it in different condition. |
</p> | </p> | ||
<div class="borderstyle"> | <div class="borderstyle"> | ||
<img class="bigimg" src="https://static.igem.org/mediawiki/2018/3/35/T--NCKU_Tainan--background_ccsu.png" alt="CCSU"> | <img class="bigimg" src="https://static.igem.org/mediawiki/2018/3/35/T--NCKU_Tainan--background_ccsu.png" alt="CCSU"> | ||
− | <p class="pcontent">In order to reduce | + | <p class="pcontent">In order to reduce GHGs emissions, |
− | industry | + | the industry have long been committed to energy saving and carbon reduction in the process. |
− | + | CO<sub>2</sub> emissions in the factory are currently moving toward the technology of Carbon Capture | |
− | Storage | + | Storage and Utilization (CCS & U). Development of CCS & U is a determine factor in reducing directly emits CO<sub>2</sub> into the atmosphere. |
</p> | </p> | ||
</div> | </div> | ||
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<p class="pcontent">After doing our research, we believed that it is possible to clone some gene fragments | <p class="pcontent">After doing our research, we believed that it is possible to clone some gene fragments | ||
that can express PRK, CA and Rubisco which is from a unicellular cyanobacteria strain, | that can express PRK, CA and Rubisco which is from a unicellular cyanobacteria strain, | ||
− | <i>Synchococcus alongatus | + | <i>Synchococcus alongatus</i> pcc 7942 and <i>Synchococcus alongatus</i> pcc 7002into our model organism, <i>E. coli</i>. |
− | After cloning | + | After cloning three gene fragments into <i>E. coli</i>, |
− | it could | + | it could build a new metabolic pathway that can accomplish carbon utilization, |
− | subverting the cognition that only most of the plants could | + | subverting the cognition that only most of the plants could utilize CO<sub>2</sub>. |
</p> | </p> | ||
<p class="pcontent">Firstly, <i>E. coli</i> will uptake xylose in the surrounding of its cultivation environment. | <p class="pcontent">Firstly, <i>E. coli</i> will uptake xylose in the surrounding of its cultivation environment. | ||
After that, xylose will be metabolized and converted into Ru5P by its own native metabolic pathway. | After that, xylose will be metabolized and converted into Ru5P by its own native metabolic pathway. | ||
− | Next, PRK will convert Ru5P into RuBP. | + | Next, PRK will convert Ru5P into RuBP. At the end, RuBP will react with CO<sub>2</sub> |
which fixed by Rubisco converted into 3-PGA and 3-PGA will become a multifunctional intermediate, pyruvate. | which fixed by Rubisco converted into 3-PGA and 3-PGA will become a multifunctional intermediate, pyruvate. | ||
</p> | </p> |
Revision as of 23:04, 17 October 2018