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− | <meta name="description" content="Wiki of Peking iGEM 2016" /> | + | <meta name="description" content="Wiki of Peking iGEM 2018" /> |
− | <meta name="author" content="Li Jiamian & Wang Yuqing"/> | + | <meta name="author" content="Peking iGEM"/> |
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− | <link rel="stylesheet" href="https://2016.igem.org/Template:Peking/css/layout?action=raw&ctype=text/css"/> | + | <link rel="stylesheet" href="https://2018.igem.org/Template:Peking/css/layout?action=raw&ctype=text/css"/> |
− | <link rel="stylesheet" href="https://2016.igem.org/Template:Peking/css/media-queries?action=raw&ctype=text/css"/> | + | <link rel="stylesheet" href="https://2018.igem.org/Template:Peking/css/media-queries?action=raw&ctype=text/css"/> |
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| <style> | | <style> |
| + | body { background: #D2D8D8 url(https://static.igem.org/mediawiki/2018/7/78/T--Peking--images_bodyBackground.jpeg); background-attachment:fixed;} |
| .texttitle{ | | .texttitle{ |
| color:#6495ED; | | color:#6495ED; |
− | font-size: 20px; | + | font-size: 28px; |
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| margin-bottom: 12px; | | margin-bottom: 12px; |
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| text-transform: uppercase; | | text-transform: uppercase; |
| font-weight: 350; | | font-weight: 350; |
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| + | #page-wrap a:hover {color:#6495ED;} |
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| #sidebar { | | #sidebar { |
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| </ul> | | </ul> |
| </li> | | </li> |
− | <li class="dropdown menu-3"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Modeling</a> | + | <li class="menu-3"><a class="colapse-menu1" href="https://2018.igem.org/Team:Peking/Model">Modeling</a> |
− | <ul class="dropdown-menu">
| + | |
− | <li><a href="https://2018.igem.org/Team:Peking/Modeling_overview">Overview</a></li>
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− | <li><a href="https://2018.igem.org/Team:Peking/SPOT_Formation" class="barfont1">SPOT Formation</a></li>
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− | <li><a href="https://2018.igem.org/Team:Peking/Application" class="barfont1">Application</a></li>
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− | </ul>
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| </li> | | </li> |
| <li class="menu-4"><a class="colapse-menu1" href="https://2018.igem.org/Team:Peking/Software">Software</a> | | <li class="menu-4"><a class="colapse-menu1" href="https://2018.igem.org/Team:Peking/Software">Software</a> |
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− | <li class="dropdown menu-6"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Human Practices</a>
| + | <li class="menu-6"><a class="colapse-menu1" href="https://2018.igem.org/Team:Peking/Human_Practices">Human Practices</a> |
− | <ul class="dropdown-menu">
| + | </li> |
− | <li><a href="https://2018.igem.org/Team:Peking/Human_Practices" class="barfont1">Overview</a></li>
| + | <li class="dropdown menu-7"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Achievement</a> |
− | <li><a href="https://2018.igem.org/Team:Peking/Statistics" class="barfont1">Statistics</a></li>
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− | <li><a href="https://2018.igem.org/Team:Peking/Public_Engagement" class="barfont1">Public Engagement</a></li>
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− | <li><a href="https://2018.igem.org/Team:Peking/Other" class="barfont1">Other</a></li>
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− | </ul>
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− | </li>
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− | <li class="dropdown menu-7"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Achevement</a> | + | |
| <ul class="dropdown-menu"> | | <ul class="dropdown-menu"> |
| <li><a href="https://2018.igem.org/Team:Peking/Judging_Form" class="barfont1">Judging Form</a></li> | | <li><a href="https://2018.igem.org/Team:Peking/Judging_Form" class="barfont1">Judging Form</a></li> |
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| <li><a href="https://2018.igem.org/Team:Peking/Collaborations" class="barfont1">Collaborations</a></li> | | <li><a href="https://2018.igem.org/Team:Peking/Collaborations" class="barfont1">Collaborations</a></li> |
| <li><a href="https://2018.igem.org/Team:Peking/Safety" class="barfont1">Safety</a></li> | | <li><a href="https://2018.igem.org/Team:Peking/Safety" class="barfont1">Safety</a></li> |
| + | <li><a href="https://2018.igem.org/Team:Peking/Acknowledgement" class="barfont1">Acknowledgement</a></li> |
| + | |
| </ul> | | </ul> |
| </li> | | </li> |
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| <div id="page-wrap"> | | <div id="page-wrap"> |
| <div id="sidebar" style="color:#000000"> | | <div id="sidebar" style="color:#000000"> |
− | <h4><a href="https://2018.igem.org/Team:Peking/Project">Description</a></h4> | + | <h4><a href="https://2018.igem.org/Team:Peking/Project">•Description</a></h4> |
− | <h4><a href="https://2018.igem.org/Team:Peking/Design">Design</a></h4> | + | <h4><a href="https://2018.igem.org/Team:Peking/Design">•Design</a></h4> |
− | <h4><a href="https://2018.igem.org/Team:Peking/Demonstration">Demonstration</a></h4> | + | <h4><a href="https://2018.igem.org/Team:Peking/Demonstration">•Demonstration</a></h4> |
− | <h4><a href="https://2018.igem.org/Team:Peking/Perspective">Perspective</a></h4> | + | <h4><a href="https://2018.igem.org/Team:Peking/Perspective">•Perspective</a></h4> |
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| <div class="nine columns"> | | <div class="nine columns"> |
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− | <div class="texttitle">More interaction modules can be used in our organelles | + | <div class="texttitle">More interaction modules can be used |
| <a id="A"></a></div> | | <a id="A"></a></div> |
| <hr style="border:2px dashed; height:2px" color="#666666"> | | <hr style="border:2px dashed; height:2px" color="#666666"> |
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| </p> | | </p> |
| <div align="center"><img src="https://static.igem.org/mediawiki/2018/a/af/T--Peking--PS_1.png"> | | <div align="center"><img src="https://static.igem.org/mediawiki/2018/a/af/T--Peking--PS_1.png"> |
− | <p style="text-align:justify; text-justify:inter-ideograph;">Figure. 1A:Design of light induced SPOT system. PhyB and PIF6 can combine in the presence of far infrared light. Figure. 1B: The potential of light induced SPOT system. Their formation and disassociation can be controlled rapidly. They can work well with chemical-induced SPOT in the same cell.</p> | + | <p style="text-align:justify; text-justify:inter-ideograph;">Figure. 1A Design of light induced SPOT system. PhyB and PIF6 can combine in the presence of far infrared light. <br/> |
| + | Figure. 1B The potential of light induced SPOT system. Their formation and disassociation can be controlled rapidly. They can work well with chemical-induced SPOT in the same cell.</p> |
| </div> | | </div> |
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| </div> | | </div> |
− | <div class="texttitle">Isolated synthetic organelles can be formed in one cell | + | <div class="texttitle">Isolated synthetic organelles can be formed |
| <a id="A"></a></div> | | <a id="A"></a></div> |
| <hr style="border:2px dashed; height:2px" color="#666666"> | | <hr style="border:2px dashed; height:2px" color="#666666"> |
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| </div> | | </div> |
| <div class="content"> | | <div class="content"> |
− | <p>We have achieved the formation of SPOT in living cells with two kinds of interaction modules respectively. It’s easy to think about what if there are several sets SPOT in just one cell. As mentioned before, there are many orthogonal dimerization system, that we can transform rapamycin induced, plant hormone-induced, light induced, and other kinds of SPOTs into one strain of yeast. We hope they can co-exist and can be induced and perform functions independently. (Figure. 1B) | + | <p>We have achieved the formation of SPOT in living cells with two kinds of interaction modules respectively. It’s easy to think about what if there are several sets SPOT in just one cell. As mentioned before, there are many orthogonal dimerization system, that we can transform rapamycin induced, plant hormone-induced, light induced, and other kinds of SPOTs into one strain of yeast. We hope they can co-exist and can be induced and perform functions independently. (Fig. 1B) |
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| </p> | | </p> |
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| </p> | | </p> |
| <div align="center"> <img src="https://static.igem.org/mediawiki/2018/8/85/T--Peking--PS_2.png"></div> | | <div align="center"> <img src="https://static.igem.org/mediawiki/2018/8/85/T--Peking--PS_2.png"></div> |
− | <p style="text-align:justify; text-justify:inter-ideograph;">Figure. 2A Fused function module and recruited function module. When function modules are recruited to SPOT, they may function well. | + | <p style="text-align:justify; text-justify:inter-ideograph;">Figure. 2A Fused function module and recruited function module. When function modules are recruited to SPOT, they may function well.<br/> |
− | Figure. 2B Demonstration of nanobody system. Anti-GFP nanobody can combine to GFP and recruit the function module (replaced by CFP). The images merged well and confirmed that the design of nanobody system is feasible.</p> | + | Figure. 2B Demonstration of nanobody system. Anti-GFP nanobody can combine to GFP and recruit the function module (replaced by CFP). The images merged well and confirmed that the design of nanobody system is feasible. |
| + | <br/> |
| + | <nr/></p> |
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− | <div class="texttitle">More applications can be achieved by our SPOT | + | <div class="texttitle">More applications can be achieved |
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| <a id="B"></a></div> | | <a id="B"></a></div> |
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| <p>Phase separation processes show sensitive dynamics, and we think that our synthetic organelles can be introduced into artificial signal pathway as a signal amplifier. </p> | | <p>Phase separation processes show sensitive dynamics, and we think that our synthetic organelles can be introduced into artificial signal pathway as a signal amplifier. </p> |
| <div align="center"><img src="https://static.igem.org/mediawiki/2018/6/60/T--Peking--PS_3.png"></div> | | <div align="center"><img src="https://static.igem.org/mediawiki/2018/6/60/T--Peking--PS_3.png"></div> |
− | <p style="text-align:justify; text-justify:inter-ideograph;">Figure. 3A NAD is a key molecule in metabolism of cells. Sensing NAD in vivo is an important method to research the life process and it can be achieved by SPOT system. | + | <p style="text-align:justify; text-justify:inter-ideograph;">Figure. 3A NAD is a key molecule in metabolism of cells. Sensing NAD in vivo is an important method to research the life process and it can be achieved by SPOT system.<br/> |
| Figure. 3B In a synthetic metabolic pathway, the intermediate may be toxic to cells. By finishing the whole pathway in SPOT, the toxicity may be reduce. | | Figure. 3B In a synthetic metabolic pathway, the intermediate may be toxic to cells. By finishing the whole pathway in SPOT, the toxicity may be reduce. |
| </p> | | </p> |
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| <ul class="copyright"> | | <ul class="copyright"> |
| <!--<li>© 2014 Sparrow</li> --> | | <!--<li>© 2014 Sparrow</li> --> |
− | <li><a href="https://2018.igem.org/Team:Peking">Home</a> <a href="mailto:indigomad@pku.edu.cn">Contact</a></li> | + | <li><a href="https://2018.igem.org/Team:Peking">Home</a> <a href="mailto:pekingigem2018@126.com">Contact</a></li> |
| <span> ©2018 PEKING IGEM. All Rights Reserved.</span> | | <span> ©2018 PEKING IGEM. All Rights Reserved.</span> |
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