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− | <h4><a href="javascript:void(0);" onclick="naver('A')">Experiment</a></h4> | + | <h4><a href="javascript:void(0);" onclick="naver('A')">•Experiment</a></h4> |
− | <h4><a href="javascript:void(0);" onclick="naver('B')">Beyond Experiment</a></h4> | + | <h4><a href="javascript:void(0);" onclick="naver('B')">•Beyond Experiment</a></h4> |
| </div> | | </div> |
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| <h3>Experiment:</h3><br/><br/> | | <h3>Experiment:</h3><br/><br/> |
| <p style="text-align:justify; text-justify:inter-ideograph;"> | | <p style="text-align:justify; text-justify:inter-ideograph;"> |
− | 1. We constructed a part library that can drive protein phase separation. The library includes several dimerization modules and multivalent modules.<br/><br/> | + | 1. We constructed a <a href="https://2018.igem.org/Team:Peking/Parts"> part library </a>that can drive protein phase separation. The library includes several dimerization modules and multivalent modules.<br/><br/> |
| 2. We rationally <a href="https://2018.igem.org/Team:Peking/Design">designed a synthetic organelle</a> called SPOT (Synthetic Phase separation-based Organelle Platform) and used it to achieve multi-functions. We believed SPOT to be a useful platform for other iGEM teams to investigate protein phase separation and design synthetic organelles.<br/><br/> | | 2. We rationally <a href="https://2018.igem.org/Team:Peking/Design">designed a synthetic organelle</a> called SPOT (Synthetic Phase separation-based Organelle Platform) and used it to achieve multi-functions. We believed SPOT to be a useful platform for other iGEM teams to investigate protein phase separation and design synthetic organelles.<br/><br/> |
− | 3. The synthetic organelles could form spontaneously in yeast. They could also be induced by rapamycin. We chose different platforms according to the different functions we wanted to achieve.<br/><br/> | + | 3. The synthetic organelles could form <a href="https://2018.igem.org/Team:Peking/Demonstrate#A">spontaneously</a> in yeast. They could also be induced by rapamycin. We chose different platforms according to the different functions we wanted to achieve.<br/><br/> |
− | 4. We set up <a herf="https://2018.igem.org/Team:Peking/Model">a thermodynamic model and a kinetic model</a> to characterize our system. The experimental results were consistent with the models.<br/><br/> | + | 4. We set up <a href="https://2018.igem.org/Team:Peking/Model">a thermodynamic model and a kinetic model</a> to characterize our system. The experimental results were consistent with the models.<br/><br/> |
− | 5. We tested the strength of four different yeast promoters using flow cytometry.<br/><br/> | + | 5. We tested <a href="http://parts.igem.org/Part:BBa_K2601021#A"> the strength of four different yeast promoters </a> using flow cytometry.<br/><br/> |
− | 6. We thoroughly tested some key parameters of the paired FKBP-HOTag3 and Frb-HOTag6. The thermodynamic properties and the kinetic properties were well characterized.<br/><br/> | + | 6. We thoroughly tested some key parameters of the paired FKBP-HOTag3 and Frb-HOTag6. The<a href="https://2018.igem.org/Team:Peking/Demonstrate#C1"> thermodynamic properties </a>and the<a href="https://2018.igem.org/Team:Peking/Demonstrate#C3"> kinetic properties </a>were well characterized.<br/><br/> |
− | 7. We explored the biophysical properties of the SUMO-SIM system. We proved the granules were liquid-like.<br/><br/> | + | 7. We explored the<a href="https://2018.igem.org/Team:Peking/Demonstrate#B"> biophysical properties </a>of the SUMO-SIM system. We proved the granules were liquid-like.<br/><br/> |
− | 8. Three enzymes were essential for β-carotene producing in yeast. We loaded the three enzymes on the synthetic organelle and increased the reaction rate. We confirmed SPOT could function as a reaction crucible.<br/><br/> | + | 8. Three enzymes were essential for β-carotene producing in yeast. We loaded the three enzymes on the synthetic organelle and<a href="https://2018.igem.org/Team:Peking/Demonstrate#D3"> increased the reaction rate </a>. We confirmed SPOT could function as a reaction crucible.<br/><br/> |
− | 9. The inducible SPOT could sequester rapamycin with high efficiency. We used SPOT as a cure of the toxicity of rapamycin.<br/><br/> | + | 9. The inducible SPOT could sequester rapamycin with high efficiency. We used SPOT as<a href="https://2018.igem.org/Team:Peking/Demonstrate#C2"> a cure of the toxicity of rapamycin</a>.<br/><br/> |
− | 10. We verified the high sensibility of SPOT-based sensor system. We also built an ABA sensor.<br/><br/> | + | 10. We verified the high sensibility of<a href="https://2018.igem.org/Team:Peking/Demonstrate#D2"> SPOT-based sensor system</a>. We also built an ABA sensor.<br/><br/> |
| </p> | | </p> |
| <div class="info"> | | <div class="info"> |
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| <ul class="copyright"> | | <ul class="copyright"> |
| <!--<li>© 2014 Sparrow</li> --> | | <!--<li>© 2014 Sparrow</li> --> |
− | <li><a href="https://2018.igem.org/Team:Peking">Home</a> <a href="mailto:indigomad@pku.edu.cn">Contact</a></li> | + | <li><a href="https://2018.igem.org/Team:Peking">Home</a> <a href="mailto:pekingigem2018@126.com">Contact</a></li> |
| <span> ©2018 PEKING IGEM. All Rights Reserved.</span> | | <span> ©2018 PEKING IGEM. All Rights Reserved.</span> |
| <li><a href="http://getbootstrap.com/2.3.2/">Based on Bootstrap</a></li> | | <li><a href="http://getbootstrap.com/2.3.2/">Based on Bootstrap</a></li> |