Difference between revisions of "Team:NKU CHINA"

 
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Line 1: Line 1:
{{NKU_CHINA}}
+
{{Template:NKU_CHINA/header}}
 
<html>
 
<html>
 +
<head>
 +
<style type="text/css">
 +
.footer-w3ls ul.social-icons1 li a {
 +
  margin-right:2px!important;
 +
  padding:0px !important;
 +
}
 +
#loading {
 +
  width: 100%;
 +
  height: 100%;
 +
  background: black;
 +
  position: fixed;
 +
top:0;
 +
left:0;
 +
z-index:10000;
 +
}
 +
#loading_p {
 +
    position: fixed;
 +
    top: 0;
 +
    left: 0;
 +
    width: 100%;
 +
    height: 100%;
 +
    z-index:99999;
 +
}
  
<style>
+
.loader-ring {
/**************************************************************************************************************************************************************************************************/
+
  position: fixed;
 +
  top: 50%;
 +
  left: 50%;
 +
  margin: -120px 0 0 -120px;
 +
  width: 240px;
 +
  height: 240px;
 +
  z-index:10000;
 +
}
  
 +
.loader-ring-light {
 +
  width: 240px;
 +
  height: 240px;
 +
  -moz-border-radius: 240px;
 +
  -webkit-border-radius: 240px;
 +
  border-radius: 240px;
 +
  -moz-box-shadow: 0 4px 0 #00abf2 inset;
 +
  -webkit-box-shadow: 0 4px 0 #00abf2 inset;
 +
  box-shadow: 0 4px 0 #00abf2 inset;
 +
  animation: rotate-360 2s linear infinite;
 +
}
  
 +
.loader-ring-track {
 +
  position: absolute;
 +
  top: 0;
 +
  left: 0;
 +
  width: 240px;
 +
  height: 240px;
 +
  -moz-border-radius: 240px;
 +
  -webkit-border-radius: 240px;
 +
  border-radius: 240px;
 +
  -moz-box-shadow: 0 0 10px 4px rgba(0, 0, 0, 0.3) inset;
 +
  -webkit-box-shadow: 0 0 10px 4px rgba(0, 0, 0, 0.3) inset;
 +
  box-shadow: 0 0 10px 4px rgba(0, 0, 0, 0.3) inset;
 +
}
  
/**************************************************************************************************************************************************************************************************/
+
@keyframes rotate-360 {
/* DEFAULT WIKI SETTINGS */
+
  from {
/**************************************************************************************************************************************************************************************************/
+
    -moz-transform: rotate(0);
 
+
    -ms-transform: rotate(0);
 
+
    -webkit-transform: rotate(0);
#home_logo, #sideMenu { display:none; }
+
    -o-transform: rotate(0);
#sideMenu, #top_title, .patrollink  {display:none;}
+
    transform: rotate(0);
#content { margin-left:0px; margin-top:-7px; padding:0px; width:100%;}
+
  }
body {background-color:white; }
+
  to {
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
+
    -moz-transform: rotate(360deg);
 
+
    -ms-transform: rotate(360deg);
.judges-will-not-evaluate { border: 4px solid #e4dede; padding: 2% !important; width: 92%!important;}
+
    -webkit-transform: rotate(360deg);
 
+
    -o-transform: rotate(360deg);
 
+
    transform: rotate(360deg);
/**************************************************************************************************************************************************************************************************/
+
  }
/* MENU */
+
}
/**************************************************************************************************************************************************************************************************/
+
.fullscreenvideo {
 
+
    position: absolute;
/*this wraps the whole of the menu*/
+
    top: 70%;
.igem_2018_team_menu {
+
    left: 50%;
background-color:#c4baba;
+
    min-width: 100%;
border-left: 1px solid #c4baba;
+
    min-height: 100%;
display:block;
+
    width: auto;
float:right;
+
    height: auto;
height:100vh;
+
    z-index: -100;
max-width: 270px;
+
    -webkit-transform: translateX(-50%) translateY(-50%);
overflow-y: auto;
+
    transform: translateX(-50%) translateY(-50%);
overflow-x: hidden;
+
    -webkit-transition: 1s opacity;
padding:0px;
+
    transition: 1s opacity;
position:fixed;
+
right:0%;
+
text-align:left;
+
width: 15%;
+
}
+
 
+
 
+
.igem_2018_team_menu.displaying_menu{
+
display:block;
+
}
+
 
+
.igem_2018_team_menu  a {
+
color: #484848;
+
text-decoration:none;
+
}
+
 
+
.igem_2018_team_menu img {
+
width:100%;
+
}
+
 
+
 
+
.igem_2018_team_menu .menu_item {
+
    background-color: #c4baba;
+
    border-bottom: 1px solid #928b8b;
+
    clear: both;
+
color: #484848;
+
    cursor: pointer;
+
float: left;
+
    font-size: 120%;
+
    font-weight: bold;
+
    padding: 15px 0px 15px 5%;
+
  width: 100%;
+
}
+
 
+
.igem_2018_team_menu .menu_item.direct_link {
+
color: #484848;
+
padding-left: 15%;
+
}
+
.igem_2018_team_menu .menu_item:hover {
+
background-color: #ecb656;
+
}
+
 
+
 
+
.igem_2018_team_menu .menu_item .submenu_control_icon {
+
color: #484848;
+
float: left;
+
width: 10%;
+
}
+
 
+
/* submenu icon "+"  "-"*/
+
.igem_2018_team_menu .menu_item .submenu_control_icon::before {
+
content: "+";
+
}
+
 
+
/* submenu icon "+"  "-"*/
+
.igem_2018_team_menu .menu_item .submenu_control_icon.open::before {
+
content: "-";  
+
}
+
 
+
/*submenu wrapper*/
+
.igem_2018_team_menu .submenu{
+
background-color: #e4dede;
+
clear:both;
+
display:none;
+
float: left; 
+
width:100%;
+
}
+
 
+
/*styling for a submenu item*/
+
.igem_2018_team_menu .submenu .submenu_item {
+
border-bottom: 1px solid #c4baba;
+
  color: #635d5d;
+
    height: 30px;
+
  float: left;
+
    font-size: 110%;
+
font-weight: bold;
+
    padding: 12px 0px 0px 15%;
+
    width: 100%;
+
}
+
 
+
.igem_2018_team_menu .submenu .submenu_item:hover {
+
background-color: #f3bd5d;
+
}
+
 
+
 
+
.igem_2018_team_menu .submenu .submenu_item.current_page,
+
.igem_2018_team_menu .menu_item.current_page,
+
.igem_2018_team_menu .menu_item.direct_link.current_page {
+
background-color:#7acbd8;
+
}
+
 
+
 
+
 
+
/*mobile menu bar styling*/
+
/**************************************************************************************************************************************************************************************************/
+
 
+
 
+
.igem_2018_team_mobile_bar {
+
background-color:#e4dede;
+
border-bottom: 1px solid #c4baba;
+
cursor:pointer;
+
display:none;
+
float:left;
+
margin-top: 0;
+
padding: 5px 0;
+
position:fixed;
+
width:100%;  
+
}
+
 
+
.igem_logo_mobile img {
+
width:70px;
+
}
+
 
+
.igem_logo_mobile {
+
float:left;  
+
padding-left: 5%;
+
width: 30%;
+
}
+
 
+
.igem_menu_control_mobile img {
+
width:25px;
+
}
+
 
+
.igem_menu_control_mobile {
+
float:right;
+
padding-right:5%;  
+
padding-top:5px;
+
text-align:right;
+
width: 30%;
+
}
+
 
+
 
+
 
+
/**************************************************************************************************************************************************************************************************/
+
/* CONTENT OF THE PAGE */
+
/**************************************************************************************************************************************************************************************************/
+
 
+
/* general wrapper for the content */
+
.igem_2018_team_content {
+
background-color:white;
+
display:block;
+
width: 87%;
+
}
+
 
+
/* subwrapper to center content */
+
.igem_2018_team_content .igem_2018_team_column_wrapper {
+
margin:auto;
+
max-width: 1400px;
+
width:90%;
+
}
+
 
+
 
+
 
+
/*general styling*/
+
/**************************************************************************************************************************************************************************************************/
+
 
+
.igem_2018_team_content .igem_2018_team_column_wrapper h1 { font-size: 210%;}
+
.igem_2018_team_content .igem_2018_team_column_wrapper h2 { font-size: 190%;}
+
.igem_2018_team_content .igem_2018_team_column_wrapper h3 { font-size: 170%;}
+
.igem_2018_team_content .igem_2018_team_column_wrapper h4 { font-size: 150%;}
+
.igem_2018_team_content .igem_2018_team_column_wrapper h5 { font-size: 140%;}
+
.igem_2018_team_content .igem_2018_team_column_wrapper h6 { font-size: 130%;}
+
 
+
 
+
/* styling for the titles h1, h2*/
+
.igem_2018_team_content .igem_2018_team_column_wrapper h1, .igem_2018_team_content .igem_2018_team_column_wrapper h2 {
+
border-bottom:0px;
+
color: #635d5d;
+
font-family: "Arial Black", Gadget, sans-serif;
+
padding: 10px 0px;
+
}
+
 
+
/* styling for the titles h3, h3, h5, h6 */
+
.igem_2018_team_content .igem_2018_team_column_wrapper h3,
+
.igem_2018_team_content .igem_2018_team_column_wrapper h4,
+
.igem_2018_team_content .igem_2018_team_column_wrapper h5,
+
.igem_2018_team_content .igem_2018_team_column_wrapper h6 {
+
border-bottom:0px;
+
color: #928b8b; 
+
font-family: "Arial Black", Gadget, sans-serif;
+
padding: 5px 0px;
+
}
+
 
+
/* text */
+
.igem_2018_team_content .igem_2018_team_column_wrapper p {
+
font-size: 130%;
+
font-family: Arial, Helvetica, sans-serif;
+
padding: 5px 0px;
+
text-align: left;
+
color: #484848;
+
}
+
 
+
/* Links */
+
.igem_2018_team_content .igem_2018_team_column_wrapper a {
+
color: #5bc7d8;
+
font-weight: bold;
+
text-decoration: underline;
+
text-decoration-color:#5bc7d8;
+
transition: all 0.4s ease;
+
-webkit-transition: all 0.4s ease;
+
-moz-transition: all 0.4s ease;  
+
-ms-transition: all 0.4s ease;
+
-o-transition: all 0.4s ease;
+
}
+
 
+
/* hover for the links */
+
.igem_2018_team_content .igem_2018_team_column_wrapper a:hover {
+
color: #f8b732;
+
text-decoration:none;
+
}
+
 
+
 
+
/* Table */
+
.igem_2018_team_content .igem_2018_team_column_wrapper table {
+
border: 1px solid #928b8b;
+
border-collapse: collapse;
+
font-size: 130%;
+
width: 100%;
+
}
+
 
+
/* table cells */
+
.igem_2018_team_content .igem_2018_team_column_wrapper td {
+
border: 1px solid #c4baba;
+
border-collapse: collapse;
+
font-size: 105%;
+
padding: 10px;
+
vertical-align: text-top;
+
}
+
 
+
/* table headers */
+
.igem_2018_team_content .igem_2018_team_column_wrapper th {
+
background-color:#c4baba;
+
border: 1px solid #928b8b;
+
border-collapse: collapse;
+
font-size: 110%;
+
padding: 10px;
+
vertical-align: text-top;
+
}
+
 
+
 
+
 
+
/* non numbered lists */
+
.igem_2018_team_content .igem_2018_team_column_wrapper ul, .igem_2018_team_content .igem_2018_team_column_wrapper ol {
+
font-size: 130%;
+
font-family: Arial, Helvetica, sans-serif;
+
padding:0px 20px;
+
}
+
 
+
 
+
.igem_2018_team_content .igem_2018_team_column_wrapper ul ul li, .igem_2018_team_content .igem_2018_team_column_wrapper ul ul ul li,
+
.igem_2018_team_content .igem_2018_team_column_wrapper ul ol li, .igem_2018_team_content .igem_2018_team_column_wrapper ul ul ol li,
+
.igem_2018_team_content .igem_2018_team_column_wrapper ol ol li, .igem_2018_team_content .igem_2018_team_column_wrapper ul ol ul li,
+
.igem_2018_team_content .igem_2018_team_column_wrapper ol ul li, .igem_2018_team_content .igem_2018_team_column_wrapper ul ol ol li,
+
.igem_2018_team_content .igem_2018_team_column_wrapper ol ul ul li, .igem_2018_team_content .igem_2018_team_column_wrapper ol ol ul li,
+
.igem_2018_team_content .igem_2018_team_column_wrapper ol ol ol li, .igem_2018_team_content .igem_2018_team_column_wrapper ol ul ol li{ font-size: 76%; }
+
 
+
 
+
 
+
/*layout classes*/
+
/**************************************************************************************************************************************************************************************************/
+
 
+
/*main layout class */
+
.igem_2018_team_content .igem_2018_team_column_wrapper .column  {
+
float:left;
+
margin: 1% 2%;
+
padding: 0px;
+
}
+
 
+
/* 100% */
+
.igem_2018_team_content .igem_2018_team_column_wrapper .column.full_size { width:96%; }
+
 
+
/* 66% */
+
.igem_2018_team_content .igem_2018_team_column_wrapper .column.two_thirds_size { width: 62.6%; }
+
 
+
/* 33% */
+
.igem_2018_team_content .igem_2018_team_column_wrapper .column.third_size { width: 29.3%; }
+
 
+
 
+
 
+
 
+
/*styling for all images*/
+
.igem_2018_team_content .igem_2018_team_column_wrapper .column.full_size img,
+
.igem_2018_team_content .igem_2018_team_column_wrapper .column.two_thirds_size img,
+
.igem_2018_team_content .igem_2018_team_column_wrapper .column.third_size img {
+
margin-bottom: 15px;
+
width: 100%;
+
}
+
 
+
 
+
/* page break */
+
.igem_2018_team_content .igem_2018_team_column_wrapper .clear {
+
clear:both;
+
}
+
/*add extra space to page break with clear class*/
+
.igem_2018_team_content .igem_2018_team_column_wrapper .clear.extra_space {
+
height: 30px;
+
}
+
 
+
/* horizontal line to divide the page*/
+
.igem_2018_team_content .igem_2018_team_column_wrapper .line_divider {
+
    border-top: 1px solid #c4baba;
+
  margin: auto;
+
  width: 98%;
+
}
+
 
+
 
+
 
+
+
/*support classes*/
+
/**************************************************************************************************************************************************************************************************/
+
 
+
 
+
/*Button  */
+
/************************************************/
+
.igem_2018_team_content .igem_2018_team_column_wrapper .button_link {
+
font-size: 130%;
+
margin: 30px auto;
+
text-align: center;
+
}
+
 
+
.igem_2018_team_content .igem_2018_team_column_wrapper .button_link a  {
+
background-color: #5bc7d8;
+
color: #635d5d !important;
+
font-weight: bold;
+
margin: auto;
+
text-decoration: none !important;
+
padding: 10px 15px;
+
}
+
 
+
.igem_2018_team_content .igem_2018_team_column_wrapper .button_link a:hover {
+
background-color: #f8b732 !important; 
+
}
+
 
+
 
+
 
+
.igem_2018_team_content .igem_2018_team_column_wrapper .highlight {
+
padding: 15px 20px;
+
}
+
 
+
.igem_2018_team_content .igem_2018_team_column_wrapper .highlight p,
+
.igem_2018_team_content .igem_2018_team_column_wrapper .highlight h1,
+
.igem_2018_team_content .igem_2018_team_column_wrapper .highlight h2,
+
.igem_2018_team_content .igem_2018_team_column_wrapper .highlight h3,
+
.igem_2018_team_content .igem_2018_team_column_wrapper .highlight h4,
+
.igem_2018_team_content .igem_2018_team_column_wrapper .highlight h5,
+
.igem_2018_team_content .igem_2018_team_column_wrapper .highlight h6 {
+
padding: 5px 15px;
+
}
+
 
+
.igem_2018_team_content .igem_2018_team_column_wrapper .highlight.decoration_background {
+
background-color: #e4dede;
+
}
+
 
+
.igem_2018_team_content .igem_2018_team_column_wrapper .highlight.decoration_A_top {
+
    border-top: 4px solid #5bc7d8;
+
}
+
 
+
 
+
.igem_2018_team_content .igem_2018_team_column_wrapper .highlight.decoration_A_full {
+
    border: 4px solid #5bc7d8;
+
}
+
 
+
.igem_2018_team_content .igem_2018_team_column_wrapper .highlight.decoration_B_top {
+
    border-top: 4px solid #f8b732
+
}
+
 
+
 
+
.igem_2018_team_content .igem_2018_team_column_wrapper .highlight.decoration_B_full {
+
    border: 4px solid #f8b732;
+
}
+
 
+
 
+
 
+
 
+
/*mobile*/
+
/**************************************************************************************************************************************************************************************************/
+
 
+
 
+
/* 1800px  */
+
/************************************************/
+
@media only screen and (max-width: 1800px) {
+
.igem_2018_team_content { width: 85%;}
+
.igem_2018_team_menu {display:block;}
+
 
}
 
}
 +
.videocontainer{
 +
    position: fixed;
 +
    width: 100%;
 +
    height: 130%;
 +
    overflow: hidden;
 +
    z-index: -100;
 +
    top: -25%;
 +
text-align: center;
 +
}
 +
.videocontainer:before{
 +
    content: "";
 +
    position: absolute;
 +
    width: 100%;
 +
    height: 100%;
 +
    display: block;
 +
    z-index: -10;
 +
    top: 0;
 +
    left: 0;   
 +
}
 +
#PopQC_largr {
 +
display: none;
 +
}
 +
#jun {
 +
display: none;
 +
}
 +
#sihuansu {
 +
display: none;
 +
z-index: 10;
 +
}
 +
#pop_circle {
 +
  display: none;
 +
}
 +
.linkto{
 +
  position: absolute;
 +
  top:72vh;
 +
  left: 50%;
 +
  transform: translateX(-50%);
 +
  -moz-transform: translateX(-50%);
 +
  -ms-transform: translateX(-50%);
 +
  -webkit-transform: translateX(-50%);
 +
  z-index: 10;
 +
}
 +
.link2to{
 +
  transform: translateX(-50%);
 +
  -moz-transform: translateX(-50%);
 +
  -ms-transform: translateX(-50%);
 +
  -webkit-transform: translateX(-50%);
 +
  z-index: 10;
 +
}
 +
.a_link {cursor: pointer;}
 +
.btn_video {
 +
  color: white;
 +
  background: none;
 +
  text-align: center;
 +
}
 +
#zhuyetu img:hover {
 +
-webkit-transition: all 0.5s;
 +
-o-transition: all 0.5s;
 +
transition: all 0.5s;
 +
-webkit-transform: scale(1.2);
 +
    -ms-transform: scale(1.2);
 +
        transform: scale(1.2);
 +
}
 +
</style>
 +
<script type="text/javascript">
 +
$(function(){
 +
var bgVideo=document.getElementById("videohome");
 +
bgVideo.addEventListener('canplaythrough',function(){
 +
              $('#loading').css("display","none");
 +
              $('.loader-ring').css("display","none");
 +
              $('#loading_p').css("display","none");
 +
              $('#PopQC_largr').css("display","block");
 +
              $('#jun').fadeIn(4000);
 +
              $('#sihuansu').fadeIn(4000);
 +
              $('#pop_circle').css("display","block");
 +
    });
 +
});
 +
function scrollupone() {
 +
    $("html, body").animate({
 +
      scrollTop: $("#dinwei").offset().top }, {duration: 700,easing: "swing"});
 +
}
 +
function scrolluptwo() {
 +
    $("html, body").animate({
 +
      scrollTop: $("#dinwei2").offset().top }, {duration: 700,easing: "swing"});
 +
}
 +
</script>
 +
</head>
 +
<body>
 +
<div id="loading"></div>
 +
<div id="loading_p">
 +
<p style="color: white;font-family:myTitle3;font-size:30px;position:relative;top:47%;left:46%">Loading...</p>
 +
</div>
  
/* 1400px  */
+
  <div class="loader-ring">
/************************************************/
+
  <div class="loader-ring-light"></div>
@media only screen and (max-width: 1400px) {
+
  <div class="loader-ring-track"></div>
.igem_2018_team_menu .menu_item { font-size:100%;}
+
</div>
.igem_2018_team_menu .submenu .submenu_item { font-size:90%;}
+
  <div class="videocontainer">
.igem_2018_team_menu {display:block;}
+
        <div class="fullscreenvideo" >
}
+
        <video id="videohome" autoplay="autoplay" muted="muted" loop="loop">
 
+
          <source src="https://static.igem.org/mediawiki/2018/d/db/T--NKU_CHINA--home_bg.mp4" type="video/mp4">
@media only screen and (max-width: 1001px) {
+
        </video>
.igem_2018_team_menu {display:block;}
+
        </div>
}
+
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<main>
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    <img src="https://static.igem.org/mediawiki/2018/9/98/T--NKU_CHINA--PopQC_larger.gif" id="PopQC_largr" style="position:absolute;left: 28%;top: -14%;z-index: 10;width: 600px;height: auto;">
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    <img src="https://static.igem.org/mediawiki/2018/0/0e/T--NKU_CHINA--pop_circle.gif" id="pop_circle" style="position: absolute;left:34%;top: -3.5%;z-index: 10;">
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.igem_2018_team_content {width:100%; margin-left:0px;}
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.igem_2018_team_menu {display:none; margin-top: 45px; min-width:50%; width:50%;}
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.igem_2018_team_mobile_bar {display:block;}
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.igem_2018_team_content .igem_2018_team_column_wrapper .column.full_size, .igem_2018_team_content .igem_2018_team_column_wrapper .column.two_thirds_size,.igem_2018_team_content .igem_2018_team_column_wrapper .column.third_size {width:96%; }
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              <p style="text-align: center;"><a href="https://youtu.be/Gmr6zBYlpK0" style="color: white;font-size: 25px;">Cilck to watch our video on <span style="color: yellow;">YouTube</span></a></p>
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            <p style="color: white;font-size: 23px;line-height: 30px;" style="float: left;text-align: justify;">Population Quality Control (PopQC) system, is a new approach designed for biosynthesis yield enhancement, based on non-genetic cell-to-cell variation, which includes unequal cell division, different gene copy numbers, epigenetic modifications, random gene expression, volatile RNA stability, protein activity, etc.</p>
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            <p style="color: white;font-size: 23px;line-height: 30px;text-align: justify;">Because of those differences, different cells in a single colony will have considerable variations in protein and metabolite concentrations. Therefore, in cell cultures there will be both high- and low-producers, and the intrinsic low-producers might cause suboptimal ensemble biosynthesis. The elimination of low-producers can realize the efficient utilization of substrates and high yield of target products. Thus, it has been proved to be an efficient way for biosynthesis being more suitable for large-scale industrial production. In our project, PopQC was designed as a plasmid-based gene circuit in <i>Bacillus amyloliquefaciens</i> LL3, which continuously selects high-performing cells in order to further improve the yield of target metabolite— glutamate, and then the secondary metabolites— &#947;-PGA.</p>
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                    Nankai University</br>
 +
                    No.94 Weijin Road, Nankai District</br>
 +
                    Tianjin, P.R.China 300071</br>
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Latest revision as of 00:32, 18 October 2018

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Population Quality Control (PopQC) system, is a new approach designed for biosynthesis yield enhancement, based on non-genetic cell-to-cell variation, which includes unequal cell division, different gene copy numbers, epigenetic modifications, random gene expression, volatile RNA stability, protein activity, etc.

Because of those differences, different cells in a single colony will have considerable variations in protein and metabolite concentrations. Therefore, in cell cultures there will be both high- and low-producers, and the intrinsic low-producers might cause suboptimal ensemble biosynthesis. The elimination of low-producers can realize the efficient utilization of substrates and high yield of target products. Thus, it has been proved to be an efficient way for biosynthesis being more suitable for large-scale industrial production. In our project, PopQC was designed as a plasmid-based gene circuit in Bacillus amyloliquefaciens LL3, which continuously selects high-performing cells in order to further improve the yield of target metabolite— glutamate, and then the secondary metabolites— γ-PGA.

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