Difference between revisions of "Team:NKU CHINA"

 
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−
 
+
{{Template:NKU_CHINA/header}}
 
<html>
 
<html>
−
<head>  
+
<head>
−
<meta charset="utf-8">  
+
<style type="text/css">
−
<title>Homepage</title>
+
.footer-w3ls ul.social-icons1 li a {
−
<style>
+
  margin-right:2px!important;
−
top_menu_inside #bars_item {
+
  padding:0px !important;
−
  position: absolute;
+
−
  top: -30px !important;
+
−
  left: -100px !important;
+
−
  z-index: -1 !important;
+
−
  cursor: pointer;
+
 
}
 
}
−
sideMenu,
+
#loading {
−
top_title {
+
  width: 100%;
−
  display: none;
+
  height: 100%;
 +
  background: black;
 +
  position: fixed;
 +
top:0;
 +
left:0;
 +
z-index:10000;
 
}
 
}
−
content {
+
#loading_p {
−
  padding: 0px;
+
    position: fixed;
−
  width: 100%;
+
    top: 0;
 +
    left: 0;
 +
    width: 100%;
 +
    height: 100%;
 +
    z-index:99999;
 
}
 
}
−
body {
+
 
−
  background-color: white;
+
.loader-ring {
−
  margin: 0px;
+
  position: fixed;
 +
  top: 50%;
 +
  left: 50%;
 +
  margin: -120px 0 0 -120px;
 +
  width: 240px;
 +
  height: 240px;
 +
  z-index:10000;
 
}
 
}
−
bodyContent h1 {
+
 
−
  font-weight: 300 !important;
+
.loader-ring-light {
 +
  width: 240px;
 +
  height: 240px;
 +
  -moz-border-radius: 240px;
 +
  -webkit-border-radius: 240px;
 +
  border-radius: 240px;
 +
  -moz-box-shadow: 0 4px 0 #00abf2 inset;
 +
  -webkit-box-shadow: 0 4px 0 #00abf2 inset;
 +
  box-shadow: 0 4px 0 #00abf2 inset;
 +
  animation: rotate-360 2s linear infinite;
 
}
 
}
−
bodyContent h1,
+
 
−
bodyContent h2,
+
.loader-ring-track {
−
bodyContent h3,
+
  position: absolute;
−
bodyContent h4,
+
  top: 0;
−
bodyContent h5 {
+
  left: 0;
−
  margin-bottom: 0px;
+
  width: 240px;
−
  border-bottom: none;
+
  height: 240px;
−
}
+
  -moz-border-radius: 240px;
−
bodyContent {
+
  -webkit-border-radius: 240px;
−
  padding-right: 0px;
+
  border-radius: 240px;
−
}
+
  -moz-box-shadow: 0 0 10px 4px rgba(0, 0, 0, 0.3) inset;
−
globalWrapper {
+
  -webkit-box-shadow: 0 0 10px 4px rgba(0, 0, 0, 0.3) inset;
−
  font-size: 100%;
+
  box-shadow: 0 0 10px 4px rgba(0, 0, 0, 0.3) inset;
−
  padding: 0px;
+
−
  margin: -10px -20px -20px -20px;
+
−
}
+
−
.navbar-collapse {
+
−
  padding-left: 0px;
+
−
}
+
−
banner {
+
−
  margin-top: 50px;
+
−
}
+
−
sideMenu {
+
−
  margin-top: 10px;
+
−
}
+
−
.navbar-nav .dropdown-menu, .navbar .dropdown-menu {
+
−
  margin-top: 0;
+
−
}
+
−
/**************************************************************** MENU ***************************************************************/ /* Wrapper for the menu */ .igem_2018_menu_wrapper {
+
−
  width: 15%;
+
−
  height: 100vh;
+
−
  position: fixed;
+
−
  right: 0%;
+
−
  padding: 0px;
+
−
  float: right;
+
−
  border-left: 1px solid #d3d3d3;
+
−
  background-color: #dddddd;
+
−
  text-align: left;
+
−
  font-family: Tahoma, Geneva, sans-serif;
+
−
  overflow-y: auto;
+
−
  overflow-x: hidden;
+
−
}
+
−
/* this hides the scrollbar to keep view consistency */ .igem_2018_menu_wrappe::-webkit-scrollbar {
+
−
  display: none;
+
−
}
+
−
/* styling for links in the menu, removes the line under text */ .igem_2018_menu_wrapper a {
+
−
  text-decoration: none;
+
−
}
+
−
/* styling for the images in the menu */ .igem_2018_menu_wrapper img {
+
−
  width: 100%;
+
−
}
+
−
/* styling for the menu buttons */ .igem_2018_menu_wrapper .menu_button {
+
−
  width: 100%;
+
−
  padding: 10px 0px 10px 15px;
+
−
  float: left;
+
−
  border-bottom: 1px solid #d3d3d3;
+
−
  font-size: 12px;
+
−
  font-weight: bold;
+
−
  color: #5e5f5f;
+
−
  cursor: pointer;
+
−
}
+
−
.igem_2018_menu_wrapper .menu_bottom_padding {
+
−
  width: 100%;
+
−
  height: 30px;
+
−
  float: left;
+
−
}
+
−
.menu_button.direct_to_page {
+
−
  padding-left: 36px;
+
−
}
+
−
.igem_2018_menu_wrapper .menu_button .expand_collapse_icon {
+
−
  width: 10%;
+
−
  float: left;
+
−
}
+
−
.igem_2018_menu_wrapper .menu_button .expand_collapse_icon::before {
+
−
  content: "+";
+
 
}
 
}
  
−
/* styling for the menu buttons on hover */ .igem_2018_menu_wrapper .menu_button:hover, .igem_2018_menu_wrapper .submenu_button:hover, .submenu_button.current_page:hover {
+
@keyframes rotate-360 {
−
  background-color: #3399ff;
+
  from {
−
  text-decoration: none;
+
    -moz-transform: rotate(0);
−
  color: #ffffff;
+
    -ms-transform: rotate(0);
 +
    -webkit-transform: rotate(0);
 +
    -o-transform: rotate(0);
 +
    transform: rotate(0);
 +
  }
 +
  to {
 +
    -moz-transform: rotate(360deg);
 +
    -ms-transform: rotate(360deg);
 +
    -webkit-transform: rotate(360deg);
 +
    -o-transform: rotate(360deg);
 +
    transform: rotate(360deg);
 +
  }
 
}
 
}
−
/* styling for the menu button when it is the current page */ .current_page {
+
.fullscreenvideo {
−
  background-color: #7fc1f7 !important;
+
    position: absolute;
−
  color: #5e5f5f !important;
+
    top: 70%;
 +
    left: 50%;
 +
    min-width: 100%;
 +
    min-height: 100%;
 +
    width: auto;
 +
    height: auto;
 +
    z-index: -100;
 +
    -webkit-transform: translateX(-50%) translateY(-50%);
 +
    transform: translateX(-50%) translateY(-50%);
 +
    -webkit-transition: 1s opacity;
 +
    transition: 1s opacity;
 
}
 
}
−
/* styling for the submenu buttons */ .igem_2018_menu_wrapper .submenu_button {
+
.videocontainer{
−
  width: 100%;
+
    position: fixed;
−
  padding: 10px 0px 10px 34px;
+
    width: 100%;
−
  float: left;
+
    height: 130%;
−
  background-color: #f2f2f2;
+
    overflow: hidden;
−
  border-bottom: 1px solid #d3d3d3;
+
    z-index: -100;  
−
  font-size: 12px;
+
    top: -25%;
−
  color: #5e5f5f;
+
text-align: center;
−
  cursor: pointer;
+
 
}
 
}
−
/* wrapper for the submenu items, they are hidden by default*/ .igem_2018_menu_wrapper .submenu_wrapper {
+
.videocontainer:before{
−
  display: none;
+
    content: "";
 +
    position: absolute;
 +
    width: 100%;
 +
    height: 100%;
 +
    display: block;
 +
    z-index: -10;
 +
    top: 0;
 +
    left: 0;   
 
}
 
}
−
/* when the page size is bigger than 800px, this show/hide control is hidden by default */ .igem_2018_menu_wrapper #display_menu_control {
+
#PopQC_largr {
−
  display: none;
+
display: none;
−
  text-align: center;
+
 
}
 
}
−
/***************************************************** CONTENT OF THE PAGE ****************************************************/
+
#jun {
−
/* Wrapper for the content */ .igem_2018_content_wrapper {
+
display: none;
−
  width: 81%;
+
−
  margin: 2%;
+
−
  display: block;
+
−
  float: left;
+
−
  background-color: white;
+
−
  font-family: Tahoma, Geneva, sans-serif;
+
 
}
 
}
−
/********************************* HTML STYLING *********************************/
+
#sihuansu {
−
/* styling for the titles h1 h2 */ .igem_2018_content_wrapper h1, .igem_2018_content_wrapper h2 {
+
display: none;
−
  padding: 5px 15px;
+
z-index: 10;
−
  border-bottom: 0px;
+
−
  color: #3399ff;
+
 
}
 
}
−
/* styling for the titles h3 h4 h5 h6*/ .igem_2018_content_wrapper h3, .igem_2018_content_wrapper h4, .igem_2018_content_wrapper h5, .igem_2018_content_wrapper h6 {
+
#pop_circle {
−
  padding: 5px 15px;
+
  display: none;
−
  border-bottom: 0px;
+
−
  color: #000000;
+
 
}
 
}
−
/* font and text */ .igem_2018_content_wrapper p {
+
.linkto{
−
  padding: 0px 15px;
+
  position: absolute;
−
  font-size: 13px;
+
  top:72vh;
 +
  left: 50%;
 +
  transform: translateX(-50%);
 +
  -moz-transform: translateX(-50%);
 +
  -ms-transform: translateX(-50%);
 +
  -webkit-transform: translateX(-50%);
 +
  z-index: 10;
 
}
 
}
−
/* Links */ .igem_2018_content_wrapper a {
+
.link2to{
−
  font-weight: bold;
+
  transform: translateX(-50%);
−
  text-decoration: underline;
+
  -moz-transform: translateX(-50%);
−
  text-decoration-color: #3399ff;
+
  -ms-transform: translateX(-50%);
−
  color: #3399ff;
+
  -webkit-transform: translateX(-50%);
−
  -webkit-transition: all 0.4s ease;
+
  z-index: 10;
−
  -moz-transition: all 0.4s ease;
+
−
  -ms-transition: all 0.4s ease;
+
−
  -o-transition: all 0.4s ease;
+
−
  transition: all 0.4s ease;
+
 
}
 
}
−
/* hover for the links */ .igem_2018_content_wrapper a:hover {
+
.a_link {cursor: pointer;}
−
  text-decoration: none;
+
.btn_video {
−
  color: #000000;
+
  color: white;
 +
  background: none;
 +
  text-align: center;
 
}
 
}
−
/* non numbered lists */ .igem_2018_content_wrapper ul {
+
#zhuyetu img:hover {
−
  padding: 0px 20px;
+
-webkit-transition: all 0.5s;
−
  font-size: 13px;
+
-o-transition: all 0.5s;
−
  font-family: Tahoma, Geneva, sans-serif;
+
transition: all 0.5s;
 +
-webkit-transform: scale(1.2);
 +
    -ms-transform: scale(1.2);
 +
        transform: scale(1.2);
 
}
 
}
−
/* numbered lists */ .igem_2018_content_wrapper ol {
+
</style>
−
  padding: 0px;
+
<script type="text/javascript">
−
  font-size: 13px;
+
$(function(){
−
  font-family: Tahoma, Geneva, sans-serif;
+
var bgVideo=document.getElementById("videohome");
 +
bgVideo.addEventListener('canplaythrough',function(){
 +
              $('#loading').css("display","none");
 +
              $('.loader-ring').css("display","none");
 +
              $('#loading_p').css("display","none");
 +
              $('#PopQC_largr').css("display","block");
 +
              $('#jun').fadeIn(4000);
 +
              $('#sihuansu').fadeIn(4000);
 +
              $('#pop_circle').css("display","block");
 +
    });
 +
});
 +
function scrollupone() {
 +
    $("html, body").animate({
 +
      scrollTop: $("#dinwei").offset().top }, {duration: 700,easing: "swing"});
 
}
 
}
−
/* Table */ .igem_2018_content_wrapper table {
+
function scrolluptwo() {
−
  width: 97%;
+
    $("html, body").animate({
−
  margin: 15px 10px;
+
      scrollTop: $("#dinwei2").offset().top }, {duration: 700,easing: "swing"});
−
  border: 1px solid #d3d3d3;
+
−
  border-collapse: collapse;
+
 
}
 
}
−
/* table cells */ .igem_2018_content_wrapper td {
+
</script>
−
  padding: 10px;
+
</head>
−
  vertical-align: text-top;
+
<body>
−
  border: 1px solid #d3d3d3;
+
<div id="loading"></div>
−
  border-collapse: collapse;
+
<div id="loading_p">
−
}
+
<p style="color: white;font-family:myTitle3;font-size:30px;position:relative;top:47%;left:46%">Loading...</p>
−
/* table headers */ .igem_2018_content_wrapper th {
+
</div>
−
  padding: 10px;
+
−
  vertical-align: text-top;
+
−
  border: 1px solid #d3d3d3;
+
−
  border-collapse: collapse;
+
−
  background-color: #f2f2f2;
+
−
}
+
−
/**********************************LAYOUT CLASSES **********************************/
+
−
/* general class for column divs */ .igem_2018_content_wrapper .column {
+
−
  padding: 10px 0px;
+
−
  float: left;
+
−
}
+
−
/* class for a full width column */ .column .full_size {
+
−
  width: 100%;
+
−
}
+
−
/* styling for images in a full width column*/ .column.full_size img {
+
−
  width: 97%;
+
−
  padding: 10px 15px;
+
−
}
+
−
/* class for a half width column */ .column.half_size {
+
−
  width: 50%;
+
−
}
+
−
/* styling for images in a half width column*/ .column.half_size img {
+
−
  width: 94.5%;
+
−
  padding: 10px 15px;
+
−
}
+
−
/********************************* SUPPORT CLASSES ********************************/
+
−
/* class that clears content below*/ .igem_2018_content_wrapper .clear {
+
−
  clear: both;
+
−
}
+
−
/* adds extra spacing when clearing content */ .igem_2018_content_wrapper .clear.extra_space {
+
−
  height: 30px;
+
−
}
+
−
/* highlight class, makes content slightly smaller */ .igem_2018_content_wrapper .highlight {
+
−
  margin: 0px 15px;
+
−
  padding: 15px 0px;
+
−
}
+
−
/* highlight class, adds a gray background */ .igem_2018_content_wrapper .highlight.gray {
+
−
  background-color: #f2f2f2;
+
−
}
+
−
/* highlight with decoration blue line on top */ .igem_2018_content_wrapper .highlight.blue_top {
+
−
  border-top: 4px solid #3399ff;
+
−
}
+
−
/* highlight with a full blue border decoration */ .igem_2018_content_wrapper .highlight.blue_border {
+
−
  border: 4px solid #3399ff;
+
−
}
+
−
/* button class */ .igem_2018_content_wrapper .button {
+
−
  max-width: 35%;
+
−
  margin: 30px auto;
+
−
  padding: 12px 10px;
+
−
  background-color: #3399ff;
+
−
  text-align: center;
+
−
  color: #ffffff;
+
−
  -webkit-transition: all 0.4s ease;
+
−
  -moz-transition: all 0.4s ease;
+
−
  -ms-transition: all 0.4s ease;
+
−
  -o-transition: all 0.4s ease;
+
−
  transition: all 0.4s ease;
+
−
}
+
−
/* styling for button on hover */ .igem_2018_content_wrapper .button:hover {
+
−
  background-color: #3399ff;
+
−
  color: #000000;
+
−
}
+
−
/***************************************************** RESPONSIVE STYLING ****************************************************/
+
−
/* IF THE SCREEN IS LESS THAN 1200PX */ @media only screen and (max-width: 1200px) {
+
−
  #content {
+
−
      width: 100%;
+
−
  }
+
−
  .igem_2018_menu_wrapper {
+
−
      width: 15%;
+
−
      right: 0;
+
−
  }
+
−
  .highlight {
+
−
      padding: 10px 0px;
+
−
  }
+
−
  .igem_2018_menu_wrapper #display_menu_control {
+
−
      display: none;
+
−
  }
+
−
  #menu_content {
+
−
      display: block;
+
−
  }
+
−
  .menu_button.direct_to_page {
+
−
      padding-left: 17px;
+
−
  }
+
−
}
+
−
/* IF THE SCREEN IS LESS THAN 800PX */ @media only screen and (max-width: 800px) {
+
−
  .igem_2018_menu_wrapper {
+
−
      width: 100%;
+
−
      height: 15%;
+
−
      position: relative;
+
−
      left: 0%;
+
−
  }
+
−
  .igem_2018_content_wrapper {
+
−
      width: 100%;
+
−
      margin-left: 0px;
+
−
  }
+
−
  .column.half_size {
+
−
      width: 100%;
+
−
  }
+
−
  .column.full_size img, .column.half_size img {
+
−
      width: 100%;
+
−
      padding: 10px 0px;
+
−
  }
+
−
  .highlight {
+
−
      padding: 15px 5px;
+
−
  }
+
−
  .igem_2018_menu_wrapper #display_menu_control {
+
−
      display: block;
+
−
  }
+
−
  #menu_content {
+
−
      display: none;
+
−
  }
+
−
  .igem_2018_menu_wrapper .menu_button .expand_collapse_icon {
+
−
      width: 5%;
+
−
  }
+
−
  .menu_bottom_padding {
+
−
      display: none;
+
−
  }
+
−
  .menu_button.direct_to_page {
+
−
      padding-left: 36px;
+
−
  }
+
−
}
+
−
/* special class that the system uses to make sure the team wants a page to be evaluated */ .judges-will-not-evaluate {
+
−
  width: 96.6%;
+
−
  margin: 5px 15px;
+
−
  display: block;
+
−
  border: 4px solid #3399ff;
+
−
  font-weight: bold;
+
−
}
+
−
.content_wrapper {
+
−
  width: 100%;
+
−
  height: 100vh;
+
−
}
+
−
ul {
+
−
    list-style-type: none;
+
−
    margin: 0;
+
−
    padding: 0;
+
−
    width: 200px;
+
−
    background-color: #f1f1f1;
+
−
    overflow:hidden;
+
−
    position: relative;
+
−
    top:0px;
+
−
    width: 100%;
+
−
}
+
−
+
−
li a {
+
−
    display: block;
+
−
    color: #000;
+
−
    padding: 8px 16px;
+
−
    text-decoration: none;
+
−
}
+
−
li {
+
−
    text-align: center;
+
−
    border-bottom: 1px solid #555;
+
−
    float:left;
+
−
}
+
−
+
  
−
li a:hover {
+
  <div class="loader-ring">
−
    background-color: #555;
+
  <div class="loader-ring-light"></div>
−
    color: white;
+
  <div class="loader-ring-track"></div>
−
}
+
</div>
−
li a.active {
+
  <div class="videocontainer">
−
    background-color: #4CAF50;
+
        <div class="fullscreenvideo" >
−
    color: white;
+
        <video id="videohome" autoplay="autoplay" muted="muted" loop="loop">
−
}
+
          <source src="https://static.igem.org/mediawiki/2018/d/db/T--NKU_CHINA--home_bg.mp4" type="video/mp4">
 +
        </video>
 +
        </div>
 +
  </div>
 +
<main>
 +
    <img src="https://static.igem.org/mediawiki/2018/9/98/T--NKU_CHINA--PopQC_larger.gif" id="PopQC_largr" style="position:absolute;left: 28%;top: -14%;z-index: 10;width: 600px;height: auto;">
 +
    <img src="https://static.igem.org/mediawiki/2018/0/0e/T--NKU_CHINA--pop_circle.gif" id="pop_circle" style="position: absolute;left:34%;top: -3.5%;z-index: 10;">
 +
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            <p style="color: white;font-size: 23px;line-height: 30px;" style="float: left;text-align: justify;">Population Quality Control (PopQC) system, is a new approach designed for biosynthesis yield enhancement, based on non-genetic cell-to-cell variation, which includes unequal cell division, different gene copy numbers, epigenetic modifications, random gene expression, volatile RNA stability, protein activity, etc.</p>
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            <p style="color: white;font-size: 23px;line-height: 30px;text-align: justify;">Because of those differences, different cells in a single colony will have considerable variations in protein and metabolite concentrations. Therefore, in cell cultures there will be both high- and low-producers, and the intrinsic low-producers might cause suboptimal ensemble biosynthesis. The elimination of low-producers can realize the efficient utilization of substrates and high yield of target products. Thus, it has been proved to be an efficient way for biosynthesis being more suitable for large-scale industrial production. In our project, PopQC was designed as a plasmid-based gene circuit in <i>Bacillus amyloliquefaciens</i> LL3, which continuously selects high-performing cells in order to further improve the yield of target metabolite— glutamate, and then the secondary metabolites— &#947;-PGA.</p>
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                    Nankai University</br>
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                    Tianjin, P.R.China 300071</br>
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Latest revision as of 00:32, 18 October 2018

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Short Video Display

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Population Quality Control (PopQC) system, is a new approach designed for biosynthesis yield enhancement, based on non-genetic cell-to-cell variation, which includes unequal cell division, different gene copy numbers, epigenetic modifications, random gene expression, volatile RNA stability, protein activity, etc.

Because of those differences, different cells in a single colony will have considerable variations in protein and metabolite concentrations. Therefore, in cell cultures there will be both high- and low-producers, and the intrinsic low-producers might cause suboptimal ensemble biosynthesis. The elimination of low-producers can realize the efficient utilization of substrates and high yield of target products. Thus, it has been proved to be an efficient way for biosynthesis being more suitable for large-scale industrial production. In our project, PopQC was designed as a plasmid-based gene circuit in Bacillus amyloliquefaciens LL3, which continuously selects high-performing cells in order to further improve the yield of target metabolite— glutamate, and then the secondary metabolites— γ-PGA.

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Nankai University
No.94 Weijin Road, Nankai District
Tianjin, P.R.China 300071

Copyright © Team NKU_CHINA 2018

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